Re: [Histonet] Peroxidase Stain
I have long used the kits from Sigma and found them to be extremely reliable.
Call their tech helpline for advice, or reach out to your pathologist for their
preference. I know that "homemade" saves money over kits, but for me, my most
costly and precious resource is my tech time. Save yourself and your techs a
headache and call Sigma-Aldrich.
My 2 cents, Terri
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
Honesty
AccouNtability
AgiLity
CoLlaboration
CoMpassion
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Subject: [EXTERNAL] Histonet Digest, Vol 235, Issue 9
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Contents of Histonet digest..."
Today's Topics:
1. Peroxidase stain on peripheral smears (Kendra Beechie ND-Bismarck)
2. MOHS staining (Ken M)
3. Re: Peroxidase stain on peripheral smears (John Kiernan)
--
Message: 1
Date: Mon, 12 Jun 2023 14:22:08 -0500
From: Kendra Beechie ND-Bismarck
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peroxidase stain on peripheral smears
Message-ID:
Content-Type: text/plain; charset="utf-8"
Hello,
I am seeking some guidance in regards to a peroxidase stain. We have always
used the Kaplow Method to stain peripheral blood smears, and up until recently,
we have had no issues with getting it to work. However, no matter what we try
in recent months, we have not been able to get it to work. Several different
techs have tried it and we have ordered new reagents, but there are no granules
to be seen
Does anyone have any experience with peroxidase kits? I can see that
Sigma-Aldrich has several available but I'm not sure what to go with
Any and all help would be greatly appreciated!
Thank you,
Kendra Beechie
MLS/HTL (ASCP), Technical Consultant
CHI St. Alexius Health
Bismarck, ND
Caution: This email is both proprietary and confidential, and not intended for
transmission to (or receipt by) any unauthorized person(s). If you believe that
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kindly reply to the sender stating that you have received the message in error.
Then destroy it and any attachments. Thank you.
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Message: 2
Date: Mon, 12 Jun 2023 20:34:52 +
From: Ken M
To: "histonet@lists.utsouthwestern.edu"
Subject: [Histonet] MOHS staining
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
For those doing MOHS staining either manually or automatically. How many
staining cups do you really use? Are six enough if there is a running water cup
nearby or do you need more?
Ken
--
Message: 3
Date: Tue, 13 Jun 2023 04:46:26 +
From: John Kiernan
To: "histonet@lists.utsouthwestern.edu"
,Kendra Beechie ND-Bismarck
Subject: Re: [Histonet] Peroxidase stain on peripheral smears
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
What is the Kaplow method? I can't find it in textbooks. A quick Google search
brings up only junk papers indicating that a Kaplov method may use carcinogenic
benzidine (with wrong spelling) as the chromogen.
There are simple, safe and inexpensive methods for histochemical localization
of sites of peroxidase activity.in tissue sections or smears of cells. Buy a
textbook for about $50, read it, and save your lab a fortune in buying special
kits for very simple routine histochemical jobs.
My ten cents-worth. June 2023
John Kiernan
J. A. Kiernan MB, ChB, PhD, DSc
Professor Emeritus, Anatomy & Cell Biology University of Western Ontario,
London, Canada
https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html
Also Secretary, Biological Stain Commission, Inc.
https://biologicalstaincommission.org
= = =
From: Kendra Beechie ND-Bismarck via Histonet
Sent: June 12, 2023 3:22 PM
To: histonet@lists.utsouthwestern.ed
Re: [Histonet] Peroxidase stain on peripheral smears
What is the Kaplow method? I can't find it in textbooks. A quick Google search brings up only junk papers indicating that a Kaplov method may use carcinogenic benzidine (with wrong spelling) as the chromogen. There are simple, safe and inexpensive methods for histochemical localization of sites of peroxidase activity.in tissue sections or smears of cells. Buy a textbook for about $50, read it, and save your lab a fortune in buying special kits for very simple routine histochemical jobs. My ten cents-worth. June 2023 John Kiernan J. A. Kiernan MB, ChB, PhD, DSc Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = From: Kendra Beechie ND-Bismarck via Histonet Sent: June 12, 2023 3:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From: Kendra Beechie ND-Bismarck via Histonet Sent: June 12, 2023 3:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peroxidase stain on peripheral smears
Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
