[Histonet] RE: Eosin Shades
I think that the nearest to what you wish is Eosin - Phloxine Stain. Here is the web page where you can find the formula. http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/ I use it with very good results. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Eosin Shades
Read this page about eosin counterstaining on StainsFile. Bryan Llewellyn Cesar Francisco Romero wrote: I think that the nearest to what you wish is Eosin - Phloxine Stain. Here is the web page where you can find the formula. http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/ I use it with very good results. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Eosin Shades
Silly me: http://stainsfile.info/StainsFile/stain/hematoxylin/h-and-e-eo.htm Bryan Bryan Llewellyn wrote: Read this page about eosin counterstaining on StainsFile. Bryan Llewellyn Cesar Francisco Romero wrote: I think that the nearest to what you wish is Eosin - Phloxine Stain. Here is the web page where you can find the formula. http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/ I use it with very good results. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin Leaching
I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss. Thanks in advance for your help. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, June 23, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) -- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly tony.rei...@health.qld.gov.au Subject: RE: [Histonet] Eosin Leaching To: Rathborne, Toni toni.rathbo...@rwjuh.edu, Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson' rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au Content-Type: text/plain; charset=iso-8859-1 Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] RE: Eosin Leaching
Have you changed your reagent alcohol or has the company you buy from changed where it purchases the reagent alcohol? Reagent alcohols with higher volumes of isopropanol than 5% can cause the eosin to leach out. It will leave a green powder in the bottom of you staining dish. Methanol and Isopropanol should be 5% each with 90% ethanol for the safest blend and what most of us old folks always thought reagent alcohol would stay. We were wrong and different companied can change vendors or sources and get something they were not expecting too. If you look up reagent alcohol or SDA reagent alcohol you find it has many formulas and the only real requirement by the ATF is to make the alcohol non-drinkable or poison to humans. I have seen formulas with 5 to 8% methanol and isopropanol with as low as 87% ethanol. The percentage will depend on price as they have no idea at the manufacturing level what we use ti for or why. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos, Lori (VDH) Sent: Monday, June 23, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Eosin Leaching I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss. Thanks in advance for your help. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, June 23, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) -- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly tony.rei...@health.qld.gov.au Subject: RE: [Histonet] Eosin Leaching To: Rathborne, Toni toni.rathbo...@rwjuh.edu, Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson' rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au Content-Type: text/plain; charset=iso-8859-1 Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem
[Histonet] RE: Eosin Leaching
Hi Adrienne, We see eosin leaching in our summer months when it is very humid in our lab. The humidity is 70% for most of the summer. We change out all of our alcohols daily to try and prevent it but we sometimes see it even with doing that. Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: June-23-14 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) -- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly tony.rei...@health.qld.gov.au Subject: RE: [Histonet] Eosin Leaching To: Rathborne, Toni toni.rathbo...@rwjuh.edu, Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson' rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au Content-Type: text/plain; charset=iso-8859-1 Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
[Histonet] RE: Eosin not working for xylene free HE
This exact reason is how I convinced my lab to go back to xylene!! I figure it's because there is water in your substitute. Because you can't see if there is water in it (from the air moisture or wherever...) it becomes frusterating and annoying to have to change out to fresh solutions sometimes several times a day!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Wednesday, March 27, 2013 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin not working for xylene free HE Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin not working for xylene free HE
We use hot detergent solution for dewax and a xylene substitute (Pro-Par) after staining without problems. If there is water accumulating in the clearant, it can be removed with a molecular sieve placed in the staining trays. We don't have that problem here in Montana because it is dry dry dry. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, March 27, 2013 8:11 AM To: Gerard Spoelstra; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Eosin not working for xylene free HE This exact reason is how I convinced my lab to go back to xylene!! I figure it's because there is water in your substitute. Because you can't see if there is water in it (from the air moisture or wherever...) it becomes frusterating and annoying to have to change out to fresh solutions sometimes several times a day!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Wednesday, March 27, 2013 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin not working for xylene free HE Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin intensity
Ceri, There are several ways to darken the eosin counterstain: 1. Stain longer in eosin, though with some eosins including eosin Y, this might not necessarily darken the staining since the eosin Y is a yellow red, eosin B seems to be more bluish red 2. Under-differentiate the haematoxylin, though this can result in overly stained nuclei (especially those of plasma cells). 3. Include a darker red dye in your eosin stain eg Erythrosine or even phloxine (not quite as dark). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ceri Allen Sent: Wednesday, 23 January 2013 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin intensity Dear All, Please can I ask what everyone uses as a standard Eosin recipe? The new pathologist in our department has asked for the stain to be darker, 'like the hospital does it'. I work on the research side of a vet school, so I'm not sure what histology departments in hospitals normally use. Many thanks Ceri Research Technician School of Veterinary Medicine and Science University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: eosin in the processor
Supposedly eosin used to color small specimens, or put in the processor, fluoresces enough in tissue sections to interfere with fluorescent stains. I've seen safranin O (from the microbiology lab) used to mark small surgical specimens like GI biopsies. I don't know whether you can put it in the processor or not. Supposedly safranin O isn't fluorescent, though I haven't verified that. Bob Richmond Samurai Pathoogist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: ] Eosin and the Peloris
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, September 09, 2012 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 106, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu If you don't want eosin in the alcohols, try tinting your formalin with hematoxylin. When I worked at LSU, one of the techs put hematoxylin in the formalin that the tissues sat in before going on the processor, after grossing. It gave a light tint to the tissue, but did not interfere with anything else. It was not applied directly to the tissue, just the formalin used that day. Eosin in the formalin would not work, a more water based stain would. Hope that helps. Toysha Message: 1 Date: Sat, 8 Sep 2012 11:35:46 -0700 From: Madeleine Huey madeleineh...@gmail.com Subject: [Histonet] Re: Histonet Digest, Vol 106, Issue 7 To: histonet@lists.utsouthwestern.edu Message-ID: CAF2e4CJymDDxKf_J3bSOOnU0BdBOH0E=04op3ogq59m2hlo...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Message: 3 Date: Fri, 7 Sep 2012 10:22:26 -0700 (PDT) From: angela smith we3smi...@yahoo.com Subject: [Histonet] Eosin and the Peloris To: histonet@lists.utsouthwestern.edu Message-ID: 1347038546.72102.yahoomailclas...@web125405.mail.ne1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 I know the original Peloris states that eosin should not be used in the alcohols It does say you can put it in the formalins. What are you all doing out there to mark your small needle bx and gi bx's? We prefer methods where we add to the reagents on the processor. We have tried just formalin with eosin and eosin phlox. and it does not do a very good job. thanks Angela, We add Eosin in our most purity alcohol bottles (2 bottles with ~ 95% - 100% ETOH) they work great. I do not recommend it in Formalin, because the Eosin tend to wash out during the dehydration process. No loss of small biopsies in my lab yet. Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX Histology) madelein...@elcaminohospital.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin
I just wanted to thank everyone who has responded to my message. All of the input is greatly appreciated. This is a great site to be utilized in our quest for Histology perfection. Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com From: Hannen, Valerie Sent: Thursday, August 09, 2012 11:23 AM To: histonet Subject: Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you =___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
I can still hear one of our pathologist's coming down the hall, loudly complaining that SOMETHING'S WRONG WITH THE EOSIN!! We added new, we changed to completely new, we played with the times...all to no avail... Then we changed the hematoxylin..down the hall she cameYOU FIXED THE EOSIN!!! THANK YOU!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sarah Dysart [sdys...@mirnarx.com] Sent: Thursday, August 09, 2012 11:27 AM To: Hannen, Valerie; histonet Subject: [Histonet] RE: Eosin Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
We add our own 1% phloxine in 95% alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, August 09, 2012 10:28 AM To: Hannen, Valerie; histonet Subject: [Histonet] RE: Eosin Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
How about the thickness of the tissue? Once we were cutting skins at 3 microns and the pathologist complained the Eosin was too light so we started cutting them at 4-5 microns. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 11:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Eosin
Wanda makes an excellent point, thinner sections have less contrast. Geoff On 8/9/2012 2:50 PM, wanda.sm...@hcahealthcare.com wrote: How about the thickness of the tissue? Once we were cutting skins at 3 microns and the pathologist complained the Eosin was too light so we started cutting them at 4-5 microns. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 11:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
We have used a mixture of Eosin Y and Phloxine B with acetic acid as follows: 780mL 95 %ETOH 100mL Eosin 10mL Phloxine B 4mL glacial acetic acid We seem to be consistent. We have used this for 17 years. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [valerie.han...@parrishmed.com] Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin staining for small biopsies
We use eosin in our last 100% alcohol on the processor. It is a great help for our person embedding to be able to see and orient the small biopsies. We have been doing this for years and have never had any problem with the processor or any subsequent staining that has been performed. We also at times ink translucent esophageal biopsies at the grossing station to help in embedding. This also has never presented a problem. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol Sent: Wednesday, June 20, 2012 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin staining for small biopsies Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin staining for small biopsies
We also add liquid eosin (~75mL) to our dirtiest 100% to help aide the sight of the small biopsies for embedding. You are correct though, that eosin does fluoresce. I understand why you would be concerned about FISH, so wanted to share this. I have heard from fellow techs in the field that use a common bluing agent (used for laundry!) called Mrs. Stewart's Concentrated Liquid Bluing (found locally at a discount store called Meijers). It is applied at the grossing board. These techs actually use it for skin, but the bluing withstands the processor and helps them embed the skins much easier by showing the epidermis. I do believe that this harmless liquid does not fluoresce. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Freeman, Carol [carol.free...@utoledo.edu] Sent: Wednesday, June 20, 2012 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin staining for small biopsies Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin staining for small biopsies
Here is a paper I found: Capillary Electrophoresis Artifact Due to Eosin Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005 Our molecular people are studying this issue to see how big of a problem it is. Tim Morken Supervisor, Electron Microscopy Department of Pathology UC San Francisco Medical Center tim.mor...@ucsfmedctr.org Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol Sent: Wednesday, June 20, 2012 6:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin staining for small biopsies Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin staining for small biopsies
Carol Freeman asks about using eosin and other dyes to mark small specimens for better recovery during embedding. I've found safranin O to be the best of these. Use the Gram stain counterstain used in microbiology. You mark the specimens directly, on those blue biopsy pads. It doesn't dissolve out, and supposedly it isn't fluorescent and doesn't interfere with fluorescence procedures such as FISH. - The disadvantage is that marking the specimens with it is time-consuming. I'd want it used in conjunction with a grossing log sheet that the embedder checks while embedding (dream on). Eosin is easy to put in the processor, but its fluorescence supposedly precludes its use, a serious drawback. Hematoxylin - I have no experience with it. It isn't fluorescent, but in some situations can quench fluorescence. Mrs. Stewart's Bluing - No experience with it. It's made by the same company that makes Davidson marking inks. I don't know if it's particulate. Davidson's marking inks: They do the job, but as a pathologist I find particulate material distracting when it's on the slide with a small biopsy specimen, so I'd rather they not be used, though obviously they're necessary for evaluating surgical margins on breast and skin cancer resection specimens. Only a minority of pathology labs do small specimen marking of any kind, but I think it's a good idea. I don't know of any refereed literature on this subject - don't know if it's covered in any of the standard books - I'm away from my library right now. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin to dye small Biopsies
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Eosin to dye small Biopsies
As much as I respect Dr. Richmond, I would have to disagree that staining bx's with eosin is a waste of pathologist time. It helps the embedding tech and cutting tech see the minute pieces, which may be otherwise lost. Sometimes that is the diagnostic material. We would not want to put a patient through another procedure because we couldn't recover the tissue submitted. We use a vial with a dropper. Once the biopsy is placed in the cassette you take 1 second more to drop a drop of eosin on the specimen. Well worth everyone's time in my humble opinion. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Robert Richmond rsrichm...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/22/2010 10:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Eosin to dye small Biopsies Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Eosin to dye small Biopsies
We have our grossing staff cover all GI biopsies, breast biopsies and endometrial curettings or any small colorless tissue with 0.1% basic fuchsin. It leaves it a nice pinkish tint after processing, the specimens are visible in the paraffin, and we haven't had any pathologist complaints. On Fri, Oct 22, 2010 at 8:10 AM, dkb...@chs.net wrote: As much as I respect Dr. Richmond, I would have to disagree that staining bx's with eosin is a waste of pathologist time. It helps the embedding tech and cutting tech see the minute pieces, which may be otherwise lost. Sometimes that is the diagnostic material. We would not want to put a patient through another procedure because we couldn't recover the tissue submitted. We use a vial with a dropper. Once the biopsy is placed in the cassette you take 1 second more to drop a drop of eosin on the specimen. Well worth everyone's time in my humble opinion. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Robert Richmond rsrichm...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/22/2010 10:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Eosin to dye small Biopsies Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin to dye small Biopsies
We put it in our 95% Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, October 21, 2010 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin to dye small Biopsies Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin leaching out of sections
Hi Scott, Your discription of eosin leaching out after a few days is classic for the possibility of bleach contamination on your staining containers. There are multiple possibilities of other causes, but this could be a simple cause, easily solved. Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin and Leica XL stainer
Cheryl, Yes, the Lecia XL is supposed to vibrate when it lifts the racks. If it's not doing that, you need to call and ask for some assistance. It sounds to me like there is something either turned off or broken in the setup. Regarding the eosin getting the alcohol pink, that happens to us all the time. We go from eosin to 95%, 3-100% and then 3-xylenes before coverslipping. It doesn't seem to affect the quality of our staining. hopper _ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/196390709/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re; eosin
Thanks for all your help. It makes sense that it would evaporate faster on the Leica as opposed to hand staining. The eosin on the Leica is uncovered for 6 plus hours a day when hand staining it was covered except when we were staining a rack. I will add 100 alcohol as needed. Again thanks for all your help. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin evaporation.
Hi Cheryl, Maybe the auto stainer does not drain the rack a well or it could just be a matter of the dipping and dunking that is causing more evaporation. Jan Omaha, NÉE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, January 15, 2010 9:06 AM To: histonet; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] Eosin evaporation. I recently purchased a leica refurbished autostainer, Before we hand stained our by hand. Now I notice the eosin is getting very low very fast, we have to replenish almost everyday. Before a bottle of Richard Allen Eosin would last the week or at least six days. Any idea's? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Eosin evaporation.
Eosin is alcohol-based, thus the evaporation. The containers on my autostainer get covered as soon as the last run is finished and that slows evaporation somewhat but I do replenish as needed. I like my eosin level in the container a little lower so we don't have pink-stained frosted ends, but that's just me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Eosin evaporation.
I agree Sally; we cover as soon as the run is over and had to adjust the level of our H E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. Peg -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, January 15, 2010 10:22 AM To: Mahoney,Janice A; Cheri Miller; histonet; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. Eosin is alcohol-based, thus the evaporation. The containers on my autostainer get covered as soon as the last run is finished and that slows evaporation somewhat but I do replenish as needed. I like my eosin level in the container a little lower so we don't have pink-stained frosted ends, but that's just me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Eosin evaporation.
We do this as well, that is why I am stumped. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: Sherwood, Margaret [mailto:msherw...@partners.org] Sent: Friday, January 15, 2010 9:53 AM To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. I agree Sally; we cover as soon as the run is over and had to adjust the level of our H E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Eosin evaporation.
Cheri, How close on your stain line is your eosin in relation to the oven? It's possible if it nearby, the oven is evaporating your eosin more rapidly than expected. Just a thought. Sheila Haas Laboratory Supervisor Micro Path Laboratories From: Cheri Miller cmil...@physlab.com To: Sherwood, Margaret msherw...@partners.org; Breeden, Sara sbree...@nmda.nmsu.edu; Mahoney, Janice A janice.maho...@alegent.org; histonet histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Sent: Fri, January 15, 2010 11:14:30 AM Subject: RE: [Histonet] RE: Eosin evaporation. We do this as well, that is why I am stumped. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: Sherwood, Margaret [mailto:msherw...@partners.org] Sent: Friday, January 15, 2010 9:53 AM To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. I agree Sally; we cover as soon as the run is over and had to adjust the level of our H E since the frosted end of the slides were getting stained as well. We also try to limit the sections to the bottom half or third of the sldie. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re; eosin
I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Also the alcohol after eosin is pretty pink after only a few racks. Tell me what you think...thanks, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] re; eosin
I think that you should talk with the person who sold you this refurbished instrument and present your problem. That will be more expeditious. René J. --- On Fri, 1/15/10, Cheri Miller cmil...@physlab.com wrote: From: Cheri Miller cmil...@physlab.com Subject: [Histonet] re; eosin To: histonet histonet@lists.utsouthwestern.edu Date: Friday, January 15, 2010, 1:04 PM I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Also the alcohol after eosin is pretty pink after only a few racks. Tell me what you think...thanks, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] re; eosin
I never noticed if ours shakes to rid the excess, but I do know the 95% alcohol after eosin is pink but even when we stained manually, we had that as well. What we do with the XL is rotate the 2nd 95% to that position and put fresh 95% in the 2nd position. However, we only do that daily, not after each run. Even manually, we made that switch daily, not after each run. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, January 15, 2010 1:04 PM To: histonet Subject: [Histonet] re; eosin I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Also the alcohol after eosin is pretty pink after only a few racks. Tell me what you think...thanks, Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin in alcohol
We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail® has ever-growing storage! Don’t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Eosin in alcohol
We use eosin on the gross bench. We keep a bottle of concentrated Eosin and Hematoxylin on the bench with an eyedropper. The eosin is for endoscopic biopsies and the hematoxylin is for prostate biopsies. Neither has never interfered with IHC staining. Debbie M. Boyd, HT(ASCP),Chief Histologist, Southside Regional Medical Center, 200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F: 804-765-5582, dkb...@chs.net Jennifer Johnson jmjohnso...@hotmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/01/2009 01:09 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail® has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Eosin in alcohol
We use about the same amount or eosin in the first 100% alcohol and also do immunos on the biopsies and have no problem. We do all GI biopsies though not prostate. I'm not sure why the eosin would interfere with the immuno staining since it disappears during the hydration and retrieval process of staining immunos. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Wednesday, July 01, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Eosin in alcohol
Jennifer, is it possible that the lab asking you to use cobalt blue is using a fluorescence technique on the prostates? We've used eosin for years and not seen any impact on IHC staining for light microscopy. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Wednesday, July 01, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Eosin in alcohol
I think they are probably getting weak unwanted staining with P504s/AMACR antibody on benign glands on their PIN4s. Since this antibody is usually pink/red, it sounds like someone might have decided that the unwanted color came from eosin in the processor, but this would be the wrong conclusion. Eosin washes out. Mark Tarango HT(ASCP)QIHC On Wed, Jul 1, 2009 at 10:06 AM, Jennifer Johnson jmjohnso...@hotmail.comwrote: We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail® has ever-growing storage! Don’t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin in alcohol
I have never heard of eosin interfering with IHC, as a matter of fact, I did use it regularly and never had a problem with the IHC. Perhaps there was another cause for the problems with the IHC. rené J. --- On Wed, 7/1/09, Jennifer Johnson jmjohnso...@hotmail.com wrote: From: Jennifer Johnson jmjohnso...@hotmail.com Subject: [Histonet] Re: Eosin in alcohol To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 1, 2009, 1:06 PM We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail® has ever-growing storage! Don’t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Eosin in alcohol
Are the prostate biopsies, fixed in formalin or Bouins? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda [lbla...@digestivespecialists.com] Sent: Wednesday, July 01, 2009 1:47 PM To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Eosin in alcohol We use about the same amount or eosin in the first 100% alcohol and also do immunos on the biopsies and have no problem. We do all GI biopsies though not prostate. I'm not sure why the eosin would interfere with the immuno staining since it disappears during the hydration and retrieval process of staining immunos. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Wednesday, July 01, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _ Hotmail(r) has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
