I assumed distilled water?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 E= ast Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] Re: Cryoprotection
From: Mejia, Maria [1]Maria.Meji= a...@ucsf.edu
Date: Tue, August 24, 2010 10:31 am
To: vaigunda ragavendran [2]rags_j= e...@yahoo.co.in,
[3]histo...@lists.utsout= hwestern.edu [4]
histonet@lists.utsouthwestern.edu
Can you be more specific tell us what DW means please give us t he
protocol for the sucrose including
the amount of DW used. This way we can help you.
Regards
Maria Mejia
Histology Supervisor
Department of Neurosurgery
UCSF
San Francisco, CA
From: [5]histonet= -boun...@lists.utsouthwestern.edu
[[6]histonet-boun...@lists.utsouthwestern.edu] On = Behalf Of vaigunda
ragavendran [[7]rag= s_...@yahoo.co.in]
Sent: Friday, August 20, 2010 1:19 PM
To: [8]histo...@lists.uts= outhwestern.edu
Subject: [Histonet] Re: Cryoprotection
Hii,
If instead of sucrose in PBS, we use sucrose in DW by mistake, what
might h= appen to fixed tissue sections during cryoprotection..
Please lemme know..
Rags,
Postdoctoral Research Fellow,
Verge Lab,
Department of Anatomy and Cell Biology,
Room No: 5800, Saskatoon City Hospital,
701, Queen Street,
Saskatoon, Saskatchewan S7K 0M7,
Canada.
Phone (Lab): +1 306-655-8714
(Facsimile): +1 306-655-8709
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References
1. 3Dmailto:maria.me...@ucsf.edu;
2. 3Dmailto:rags_...@yahoo.co.in;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:histonet-boun...@l 7. 3Dmailto:rags_...@yahoo.co.in;
8. 3Dmailto:histonet@lists.utsouthwestern.edu;
9. 3Dmailto:Histonet@lists.utsouthwestern.edu;
10. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
11. 3Dmailto:Histonet@lists.utsouthwestern.edu;
12. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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