Re: [Histonet] Recommended thickness of Amyloid sections
Depends on what you want to do: positivity or maximise signal? I have FS and Pwax images up in Histonet image archive if you care to have a look Best wishes to all Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
In response to several questions and responses about amyloid staining: I’ve always understood that sections for amyloid should be cut at 8 µm, but could never get a histotech to cut them that thick. I recall that unstained paraffin sections on the slide are usable for about a month after cutting. That limitation precludes buying commercial controls. Most (though not all) medullary carcinomas of the thyroid contain some amyloid, and that’s about the only control you can get for yourself, and it’s a rather rare tumor, and not easily recovered from a lab’s old records. Every time somebody posts on amyloid on Histonet or elsewhere I’ve asked this question, and never got the slightest reply: Amyloid (of the AL type, I suppose) can rather easily be induced in experimental animals (by injection of casein, for example). Does any pathology service use animal material for amyloid controls? Is it commercially available? In 2002 Dick Dapson at Anatech introduced “Amyloid Red” (Direct Red 72, C.I. 29200) as a substitute for Congo red. I just checked the Web site and Anatech still offers it. Does anyone have any experience with it? Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
Agree. 8-10 microns cut just prior to staining. Roxana Robinson > On Apr 16, 2020, at 3:50 PM, raestask via Histonet > wrote: > > Should also be cut on the day they are to be stained.Rae Staskiewicz > HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone > Original message From: Paula Keene Pierce via Histonet > Date: 4/16/20 3:24 PM (GMT-06:00) To: > histonet@lists.utsouthwestern.edu, Ken M Subject: Re: > [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of > being a registered HT, I was taught that slides for amyloid are to be cut at > 8-10µm.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, > Inc.5830 N Blue Lake DriveNorman, OK 73069PH > 405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without > a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM > CDT, Ken M via Histonet wrote:Hello > All. We have always cut all of our histology control slides at 5m. We were > told today that it is common practice to cut Amyloid at 8m? Is this your > experience?Ken___Histonet mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___Histonet mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
I agree, Slightly thicker makes the polarisation easier to see (personal experience). I would love to see a study comparing section thickness Vs polarisation characteristics. Anyone interested? (being a kids hospital we rarely see amyloidosis) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Paula Keene Pierce via Histonet Sent: Friday, 17 April 2020 06:24 To: histonet@lists.utsouthwestern.edu; Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Ken M via Histonet Sent: Thursday, April 16, 2020 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommended thickness of Amyloid sections Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=iORugZls2LlYyCAZRB3XLg=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA=zu56n7jP8NfTVcr03S3ER1yqzKYomhe2VIC5gz5CiHI=OTIHpP1NshAzBBAOzuP2Ljiz166ODsMhL_CDtbvQJgs= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
I work at a Dermatology lab and our protocol for amyloid is 8-10 microns. Sent from my iPhone > On Apr 16, 2020, at 4:19 PM, Ken M via Histonet > wrote: > > Hello All. We have always cut all of our histology control slides at 5m. > We were told today that it is common practice to cut Amyloid at 8m? Is this > your experience? > > Ken > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
Should also be cut on the day they are to be stained.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Paula Keene Pierce via Histonet Date: 4/16/20 3:24 PM (GMT-06:00) To: histonet@lists.utsouthwestern.edu, Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience?Ken___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recommended thickness of Amyloid sections
Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
