Re: [Histonet] Shiny side of a paraffin section
Hello Everyone I have worked in histology for 41 years and the only reason we ever put sections shiny side up is when we want to look at back to back sections: We would take the first section and put it shiny side up in the bath and the next section immediately after would be placed shiny side down. This will give you adjacent sections that will be cut through the same face i.e. if a cell is cut in half the other half will be mirrored on the other section. This can be a very handy tool for comparing IHC staining. Cheers James H Reilly Senior Histology Technician Institute Of Infection, Immunity, Inflammation College Of Medical, Veterinary and Life Sciences University Of Glasgow Room B4/27 Sir Graeme Davies Building 120 University Place Glasgow G12 8TA Tel: +44 141 330 8420/7573 The University of Glasgow is a charity registered in Scotland, charity number SC004401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Shiny side of a paraffin section
Consistency, Consistency, Consistency. 1. It is easier to place sections on the water bath with the shiny surface down (MOST times). 2. Staining-wise, for all stains that I have tried, it does not matter (H&E, PAS, Perl's Trichrome to name a few). 3. When you are matching blocks and slides prior to slides leaving the lab, it is difficult to match "rebelliously collected shiny-side up sections" with the corresponding blocks. 4. It is a favourite test of mine to have my staff and trainees look at two slides and tell me the reason for the difference (if there is any). My staff call it "Tony's Migraine Quiz" - one 'properly' collected section and the second a "rebelliously collected shiny-side up section". 4. Microscopically, when comparing special stains with a H&E, it can drive you to distraction when microscopic features do not line up. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, 29 February 2012 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shiny side of a paraffin section As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Shiny side of a paraffin section
Who wants to look at Cell bottoms all day!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Hoy Sent: Wednesday, 29 February 2012 12:36 PM To: Histonet Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === On 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as > it comes off the blade is shiny. I've always accepted it as a fact > that the shiny side always goes down on the water bath, but I've begun > to wonder why. Is there a specific reason why we're all taught to put > the shiny side down? What would the difference be between a 'properly' > collected section and a rebelliously collected shiny-side up section? Does it > even matter? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguern...@ucsd.edu > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Shiny side of a paraffin section
My mentor, Nick Roman, told me that sections adhere to the slide better if they go on shiny side down. Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says that laying the sections on the water bath or water droplet shiny side down makes it easier to remove creases. Benno Romeiss' MIKROSKOPISCHE TECHNIK and Manfred Gabe's TECHNIQUES HISTOLOGIQUES say that section should be mounted shiny side down without giving a reason. Most other authors do not even mention this matter. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Tuesday, February 28, 2012 6:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shiny side of a paraffin section As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shiny side of a paraffin section
Placing the shiny-side of the section on the water surface you assure that the sections corresponds to the block, of course you cannot turn-around the section. Also it will allow water tension to expand the section better and assures a better adhesion to the slide surface. If the section is in a ribbon you will have to decide which in the ribbon to select and you should not turn around the section. Orientation should not be an issue unless you are always going to section the block in the same way if recuts are needed. René J. --- On Tue, 2/28/12, Lucie Guernsey wrote: From: Lucie Guernsey Subject: [Histonet] Shiny side of a paraffin section To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 28, 2012, 6:56 PM As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shiny side of a paraffin section
I think it's more to have consistency, rather than, say, a physical reason. My opinion. Example: - Tech A put the shiny side down on the flotation bath, and picked up the sections on the slide, and did an H&E. - Later in the day, the pathologist needs additional levels or some special stains or IHC on the same block. - If Tech B now cuts the same block and puts shiny side up, the sections would be 180 degrees reversed. So if the pathologist saw the area of concern in lower left quadrant in the original H&E, now it would be in the upper right quadrant. Sort of the same reason when laying out ribbons, it would be nice for the the top of the block be picked up from the ribbon oriented towards the top (frosty) part of the slide. If all techs picked up the ribbon in the same orientation directions, all subsequent recuts would also be in the same direction, regardless of which tech cut the block. (Unless of course you are putting 3 ribbons on the same slide, then the top of the block may be different, but even then, the ribbons are always laid out in the same directions, so that all 3 ribbons of tissue are facing the same direction.) It just makes it easier for the pathologist to find the same area quickly on each section. And for the histotech to check the quality of the staining in specific areas on each slide. Peggy A. Wenk,HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are my own, and do not reflect upon Beaumont Hospital. -Original Message- From: Eric Hoy Sent: Tuesday, February 28, 2012 8:36 PM To: Histonet Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === On 2/28/12 5:56 PM, "Lucie Guernsey" wrote: As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shiny side of a paraffin section
The shiny side (block side) goes on the water so when you do your block to slide comparison the slide WILL match the block. We routinely perform a percentage of slide/block matches for quality control. Some organizations perform 100% slide/block match as a final QC check. Jackie O' -Original Message- From: Eric Hoy To: Histonet Sent: Tue, Feb 28, 2012 7:36 pm Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy === ric S. Hoy, Ph.D., SI(ASCP) linical Associate Professor epartment of Medical Laboratory Sciences he University of Texas Southwestern Medical Center allas, Texas mail: eric@utsouthwestern.edu == n 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shiny side of a paraffin section
All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === On 2/28/12 5:56 PM, "Lucie Guernsey" wrote: > As all of us who cut paraffin know, the underside of each section as it > comes off the blade is shiny. I've always accepted it as a fact that the > shiny side always goes down on the water bath, but I've begun to wonder > why. Is there a specific reason why we're all taught to put the shiny side > down? What would the difference be between a 'properly' collected section > and a rebelliously collected shiny-side up section? Does it even matter? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguern...@ucsd.edu > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shiny side of a paraffin section
As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
