Re: [Histonet] Warm formalin
(Instructions For Use) The paperwork that you receive with each reagent/solution/stain, etc. > On Jul 22, 2020, at 3:10 PM, Muhammad Azam wrote: > > And IFU would be what > > Sent from my iPhone > >> On Jul 22, 2020, at 3:02 PM, Lynette Pavelich via Histonet >> wrote: >> >> Carl, >> My only other thought is to do a documented study of the temperature of >> these outside storage boxes (they can be little hot/cold boxes depending on >> your location. Because, according to the inspectors, you must be able to >> assure temperature is within the required temps prior to receiving, >> including transportation. (Pre-analytical) >> So, say you do a study of the temperatures during the hottest time of the >> day of the boxes for a period of time (30 days of the hottest month(s)) to >> give an indication of a potential problem, and document. That would be one >> portion of pre-analytical. >> >> Then temperature during transportation (cars/vans can get quite hot/cold >> also needs to be addressed and documented daily just like the temperature >> logs in the labs. Many labs are getting cited for not providing good >> pre-analytical evidence. Courier services have to have temperature >> monitoring systems in place to provide as evidence. Some are quite savvy >> with alarm systems in place with monitoring. (ShipCom for example) >> >> So much documentation these days…… >> >> Lynette >> >> >>> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl wrote: >>> >>> You a re right, Lynette >>> De rigueur for Diagnostic labs! >>> My apologies for forgetting that ( I am now in research labs where...it is >>> less restricted, unfortunately). >>> Essential to be well-documented/adherent to SOPs. >>> Respectful-illy >>> >>> Carl >>> >>> >>> Carl Hobbs FIBMS >>> Histology and Imaging Manager >>> Wolfson CARD >>> Guys Campus, London Bridge >>> Kings College London >>> London >>> SE1 1UL >>> >>> >>> 020 7848 6813 >>> >>> >>> From: Lynette Pavelich >>> Sent: 22 July 2020 19:19 >>> To: Hobbs, Carl >>> Cc: histonet@lists.utsouthwestern.edu >>> Subject: Re: [Histonet] Warm formalin >>> >>> I would suggest to always refer to your reagent’s IFU insert. This will >>> advise at what temperature you should use/store. All inspectors (CAP, JC, >>> CLIA, etc.) will make you adhere to these specifications. >>> Unless you do a well documented validation study that goes outside of these >>> restrictions from the IFU that proves no patient harm, you honestly must go >>> by the IFU recommendations. This would apply to all of our >>> stains/reagents/solutions/antibodies. >>> Our world is becoming more restrictive…… >>> >>> hope this helps, >>> Lynette Pavelich, HT(ASCP), QIHC >>> >>> >>>>> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet >>>>> wrote: >>>> >>>> Depends on what you are doing with the sections. >>>> IHC or just dye -staining? >>>> Sure...too hot ( cooking) is not recommended, as stated >>>> Also stated is that high -temp fixation may also be used with no >>>> deleterious effects as long as the fixation time is not extended. >>>> However, RT -ish even for a week won't be a problem...imho >>>> Needs must? >>>> >>>> >>>> >>>> Carl Hobbs FIBMS >>>> Histology and Imaging Manager >>>> Wolfson CARD >>>> Guys Campus, London Bridge >>>> Kings College London >>>> London >>>> SE1 1UL >>>> >>>> >>>> 020 7848 6813 >>>> ___ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3Dreserved=0 >> >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
And IFU would be what Sent from my iPhone > On Jul 22, 2020, at 3:02 PM, Lynette Pavelich via Histonet > wrote: > > Carl, > My only other thought is to do a documented study of the temperature of these > outside storage boxes (they can be little hot/cold boxes depending on your > location. Because, according to the inspectors, you must be able to assure > temperature is within the required temps prior to receiving, including > transportation. (Pre-analytical) > So, say you do a study of the temperatures during the hottest time of the day > of the boxes for a period of time (30 days of the hottest month(s)) to give > an indication of a potential problem, and document. That would be one portion > of pre-analytical. > > Then temperature during transportation (cars/vans can get quite hot/cold also > needs to be addressed and documented daily just like the temperature logs in > the labs. Many labs are getting cited for not providing good pre-analytical > evidence. Courier services have to have temperature monitoring systems in > place to provide as evidence. Some are quite savvy with alarm systems in > place with monitoring. (ShipCom for example) > > So much documentation these days…… > > Lynette > > >> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl wrote: >> >> You a re right, Lynette >> De rigueur for Diagnostic labs! >> My apologies for forgetting that ( I am now in research labs where...it is >> less restricted, unfortunately). >> Essential to be well-documented/adherent to SOPs. >> Respectful-illy >> >> Carl >> >> >> Carl Hobbs FIBMS >> Histology and Imaging Manager >> Wolfson CARD >> Guys Campus, London Bridge >> Kings College London >> London >> SE1 1UL >> >> >> 020 7848 6813 >> >> >> From: Lynette Pavelich >> Sent: 22 July 2020 19:19 >> To: Hobbs, Carl >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Warm formalin >> >> I would suggest to always refer to your reagent’s IFU insert. This will >> advise at what temperature you should use/store. All inspectors (CAP, JC, >> CLIA, etc.) will make you adhere to these specifications. >> Unless you do a well documented validation study that goes outside of these >> restrictions from the IFU that proves no patient harm, you honestly must go >> by the IFU recommendations. This would apply to all of our >> stains/reagents/solutions/antibodies. >> Our world is becoming more restrictive…… >> >> hope this helps, >> Lynette Pavelich, HT(ASCP), QIHC >> >> >>>> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet >>>> wrote: >>> >>> Depends on what you are doing with the sections. >>> IHC or just dye -staining? >>> Sure...too hot ( cooking) is not recommended, as stated >>> Also stated is that high -temp fixation may also be used with no >>> deleterious effects as long as the fixation time is not extended. >>> However, RT -ish even for a week won't be a problem...imho >>> Needs must? >>> >>> >>> >>> Carl Hobbs FIBMS >>> Histology and Imaging Manager >>> Wolfson CARD >>> Guys Campus, London Bridge >>> Kings College London >>> London >>> SE1 1UL >>> >>> >>> 020 7848 6813 >>> ___ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3Dreserved=0 > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
Carl, My only other thought is to do a documented study of the temperature of these outside storage boxes (they can be little hot/cold boxes depending on your location. Because, according to the inspectors, you must be able to assure temperature is within the required temps prior to receiving, including transportation. (Pre-analytical) So, say you do a study of the temperatures during the hottest time of the day of the boxes for a period of time (30 days of the hottest month(s)) to give an indication of a potential problem, and document. That would be one portion of pre-analytical. Then temperature during transportation (cars/vans can get quite hot/cold also needs to be addressed and documented daily just like the temperature logs in the labs. Many labs are getting cited for not providing good pre-analytical evidence. Courier services have to have temperature monitoring systems in place to provide as evidence. Some are quite savvy with alarm systems in place with monitoring. (ShipCom for example) So much documentation these days…… Lynette > On Jul 22, 2020, at 2:27 PM, Hobbs, Carl wrote: > > You a re right, Lynette > De rigueur for Diagnostic labs! > My apologies for forgetting that ( I am now in research labs where...it is > less restricted, unfortunately). > Essential to be well-documented/adherent to SOPs. > Respectful-illy > > Carl > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > > > From: Lynette Pavelich > Sent: 22 July 2020 19:19 > To: Hobbs, Carl > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Warm formalin > > I would suggest to always refer to your reagent’s IFU insert. This will > advise at what temperature you should use/store. All inspectors (CAP, JC, > CLIA, etc.) will make you adhere to these specifications. > Unless you do a well documented validation study that goes outside of these > restrictions from the IFU that proves no patient harm, you honestly must go > by the IFU recommendations. This would apply to all of our > stains/reagents/solutions/antibodies. > Our world is becoming more restrictive…… > > hope this helps, > Lynette Pavelich, HT(ASCP), QIHC > > >> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet >> wrote: >> >> Depends on what you are doing with the sections. >> IHC or just dye -staining? >> Sure...too hot ( cooking) is not recommended, as stated >> Also stated is that high -temp fixation may also be used with no deleterious >> effects as long as the fixation time is not extended. >> However, RT -ish even for a week won't be a problem...imho >> Needs must? >> >> >> >> Carl Hobbs FIBMS >> Histology and Imaging Manager >> Wolfson CARD >> Guys Campus, London Bridge >> Kings College London >> London >> SE1 1UL >> >> >> 020 7848 6813 >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
You a re right, Lynette De rigueur for Diagnostic labs! My apologies for forgetting that ( I am now in research labs where...it is less restricted, unfortunately). Essential to be well-documented/adherent to SOPs. Respectful-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From: Lynette Pavelich Sent: 22 July 2020 19:19 To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warm formalin I would suggest to always refer to your reagent’s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. Our world is becoming more restrictive…… hope this helps, Lynette Pavelich, HT(ASCP), QIHC > On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet > wrote: > > Depends on what you are doing with the sections. > IHC or just dye -staining? > Sure...too hot ( cooking) is not recommended, as stated > Also stated is that high -temp fixation may also be used with no deleterious > effects as long as the fixation time is not extended. > However, RT -ish even for a week won't be a problem...imho > Needs must? > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
I would suggest to always refer to your reagent’s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. Our world is becoming more restrictive…… hope this helps, Lynette Pavelich, HT(ASCP), QIHC > On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet > wrote: > > Depends on what you are doing with the sections. > IHC or just dye -staining? > Sure...too hot ( cooking) is not recommended, as stated > Also stated is that high -temp fixation may also be used with no deleterious > effects as long as the fixation time is not extended. > However, RT -ish even for a week won't be a problem...imho > Needs must? > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
How hot are we talking about? San Francisco hot or Phoenix hot? Christopher Sheeder, HT(ASCP)CMQIHC Histology Supervisor | Department of Laboratories Seattle Children's Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.shee...@seattlechildrens.org -Original Message- From: Martin, Erin Sent: Tuesday, July 21, 2020 6:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Warm formalin Hello everyone! We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? Thanks so much! Erin Martin, Histology Supervisor UCSF Dermatopathology & Oral Pathology Service Phone: 415-3537248 | Fax: 415-353-7543 CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
Depends on what you are doing with the sections. IHC or just dye -staining? Sure...too hot ( cooking) is not recommended, as stated Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. However, RT -ish even for a week won't be a problem...imho Needs must? Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
I agree with Paula. I think the doc has a legitimate concern. Garrey Sent from my iPhone > On Jul 22, 2020, at 1:20 AM, Patpxs via Histonet > wrote: > > Hi Erin, > > Often heat is applied to formalin to speed up fixation. That said there is > probably a temperature point where it goes from fixing tissue to cooking it. > > Paula > > Sent from my iPhone > >> On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet >> wrote: >> >> Hello everyone! >> >> We have a referring clinician that is concerned about leaving his specimens >> in an outdoor lockbox in the summer because the formalin will get hot. I >> don't think that having some specimens in formalin in hot weather would >> cause any problems but I can't find any references one way or another. Does >> anyone have any policies regarding this? >> >> Thanks so much! >> >> >> Erin Martin, Histology Supervisor >> >> UCSF Dermatopathology & Oral Pathology Service >> >> Phone: 415-3537248 | Fax: 415-353-7543 >> >> >> CONFIDENTIALITY NOTICE: This email message, including any attachments, is >> for the sole use of the intended recipient(s) and may contain confidential, >> proprietary, and/or privileged information protected by law. If you are not >> the intended recipient, you may not use, copy, or distribute this email >> message or its attachments. If you believe you have received this email >> message in error, please contact the sender by reply email and destroy all >> copies of the original message. >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Warm formalin
Hi Erin, Often heat is applied to formalin to speed up fixation. That said there is probably a temperature point where it goes from fixing tissue to cooking it. Paula Sent from my iPhone > On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet > wrote: > > Hello everyone! > > We have a referring clinician that is concerned about leaving his specimens > in an outdoor lockbox in the summer because the formalin will get hot. I > don't think that having some specimens in formalin in hot weather would cause > any problems but I can't find any references one way or another. Does anyone > have any policies regarding this? > > Thanks so much! > > > Erin Martin, Histology Supervisor > > UCSF Dermatopathology & Oral Pathology Service > > Phone: 415-3537248 | Fax: 415-353-7543 > > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for > the sole use of the intended recipient(s) and may contain confidential, > proprietary, and/or privileged information protected by law. If you are not > the intended recipient, you may not use, copy, or distribute this email > message or its attachments. If you believe you have received this email > message in error, please contact the sender by reply email and destroy all > copies of the original message. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Warm formalin
Hello everyone! We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? Thanks so much! Erin Martin, Histology Supervisor UCSF Dermatopathology & Oral Pathology Service Phone: 415-3537248 | Fax: 415-353-7543 CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet