[Histonet] Hydrometer questions
Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hydrometer questions
Tim: When I (and others) wrote you about the age of the LFB I at least was referring to the age of the solution. You write now that you are going to try a 1950 LFB and I am sure that you are referring to the powder. As to powdered stains they are quite stable, but it will be nice to find out. To your hydrometer question: they are very simple to use, you just place the instrument very delicately into the ethanol sol. contained on a cylinder and it will sink up to a mark on the stem corresponding to the ethanol concentration, BUT there are some precautins to take: 1- the density of ethanol (and of any liquid for that matter) varies with the temperature; to higher temperature less push of the liquid and if the temp. is high you will get a reading corresponding to a higher ethanol concentration that the real value. 2- the cylinder has to be wide enough, otherwise the stem may come close to the wall of the cylinder and give you also a false reading. Your best solution is to prepare fresh ethanol sol. every time you need it and to do that you just calculate how much dist. water you need to add to your ethanol of KNOWN concentration. Finally, if you get a hydrometer for ethanol it cannot be used for isopropyl alcohol, because each has a different density. Hydrometers can be bought from Fisher or Thomas Adams (I think they still sell them!). René J. From: Tim Wheelock twheel...@mclean.harvard.edu To: histonet@lists.utsouthwestern.edu Sent: Friday, June 29, 2012 10:08 AM Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hydrometer temperature correction
There is a chart [ http://www.defalcos.com/tutorials/hy.html ] for temperature correction on ethanol concentration. A lot of information can be found on brewery sites. So . . if you require more help, and since its Friday, visit a local brew house to ask questions. Tresa :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, June 29, 2012 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hydrometer
CBG Biotech sells hydrometers. There item number is MIS1003.$45. 1-800-941-9484 Jana Ver Beek, HT(ASCP) Spectrum Health-Blodgett Campus Grand Rapids, MI 49506 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] hydrometer
that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradford Road Bingley BD16 1TW Switchboard: +44 1274 550600 ext 3348 Direct Dial: +44 1274 550800 http: www.ramsayhealth.co.uk http://www.ramsayhealth.co.uk/ Disclaimer and Confidentiality Note Everything in this e-mail and any attachments relating to the official business of Ramsay Health Care UK Operations Limited (Ramsay) or any of its subsidiary or associated companies is proprietary. It is confidential, legally privileged and protected by law. Ramsay does not endorse any of the content. Views and opinions are those of the sender unless clearly stated as being that of Ramsay. The person addressed in the e-mail is the sole authorised recipient. Please notify the sender immediately if it has unintentionally reached you and do not read, disclose or use the content in any way. Ramsay can not ensure that the integrity of this communication has been maintained nor that it is free of errors, virus, interception or interference. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 8 Date: Tue, 9 Mar 2010 14:08:17 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] hydrometer To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a54257bc3...@mmc-mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Anybody have any idea where to purchase a simple hydrometer for checking the water content in alcohols? I found one at TBS but do not know if it will fit our purposes since it says use with a specific recycler. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. -- Message: 9 Date: Tue, 9 Mar 2010 15:12:05 -0500 From: dkb...@chs.net Subject: Re: [Histonet] hydrometer To: Vickroy, Jim vickroy@mhsil.com Cc: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: of34202695.26373c83-on852576e1.006eea59-852576e1.006ef...@chs.net Content-Type: text/plain; charset=US-ASCII We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. -- Message: 10 Date: Tue, 9 Mar 2010 17:02:30 -0500 From: Barone, Carol cbar...@nemours.org Subject: [Histonet] Region II mtg addiiton and up-date: To: histonet@lists.utsouthwestern.edu Message-ID: 37e4bac017f57141af64faa5aeb04ce8033a7...@wlmmsx01.nemours.org Content-Type: text/plain; charset=us-ascii Histonetter's, Region II up-date: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J ...Come for a day... or come for the weekend. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach. ..(for studying, of course!). Stay tuned to the histonet, for more information regarding specific seminars and workshops. More to come... Information on registration will be posted on the histonet in April. So keep up with the histonet...for lots of great networking and info on the Region II Symposium See you there. Carol Barone - Region II Director...Oh!...and keep networking! It is the best educational tool of all! -- Message: 11 Date: Tue, 9 Mar 2010 16:27:30 -0600 From: Knutson, Deanne dknut...@primecare.org Subject: [Histonet] p16 To: 'histonet@lists.utsouthwestern.edu' histonet
Re: [Histonet] hydrometer
We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
