Re: [Histonet] large fibrous bone tissue
Louise has very good advice here as related to paraffin processing of this tissue. I may even add to soak the block a little more before taking the final sections. However, have you ever thought of processing into MMA resin? If you have these capabilities you may be very pleased with the results and find the microtomy less problematic. Let me know if or how I can be of assistance! Jack On May 15, 2010, at 4:30 AM, louise renton louise.ren...@gmail.com wrote: I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia reuel.corne...@tsrh.org wrote: How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] large fibrous bone tissue part 2
Dear Cornelia Jack i disagree about the soaking as this will negate the chilling effect of the freezer. The whole idea is to have the block as cold as possible to get maximum support from the wax*. If the tissue has been processed and fixed properly, there should not be a necessity for rehydrating on water One thing i forgot to mention wasthat when you embed, try to orientate the tissue so that the long axis (if there is one) lies in the same direction as the cutting stroke. when embedding, orientate the tissue at a slight diagonal, so that the knife dous not continously pass through the tissue on the cutting stroke - (this works well for skins also, except make sure the dermis is away from the knife) If this doesn't make sense, let me know and i will send a graphic privately *ease of sectioning relies on the embedding material being as close to the density/stiffness of the tissue being embedded. Thats why you can section undecal bone in resin best regards On Wed, May 19, 2010 at 7:33 AM, Jack Ratliff ratliffj...@hotmail.comwrote: Louise has very good advice here as related to paraffin processing of this tissue. I may even add to soak the block a little more before taking the final sections. However, have you ever thought of processing into MMA resin? If you have these capabilities you may be very pleased with the results and find the microtomy less problematic. Let me know if or how I can be of assistance! Jack On May 15, 2010, at 4:30 AM, louise renton louise.ren...@gmail.com wrote: I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia reuel.corne...@tsrh.org wrote: How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] large fibrous bone tissue
Hi Reul, It is possible that you are not processing your bone long enough in the tissue processor. If you would like to discuss this possibility, please email me. Helen Helen F Wimer HT (ASCP) Smithsonian Institution Department of Vertebrate Zoology Washington, DC (301) 496-1391 wim...@si.edu From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff [ratliffj...@hotmail.com] Sent: Wednesday, May 19, 2010 1:33 AM To: louise renton; Reuel Cornelia Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] large fibrous bone tissue Louise has very good advice here as related to paraffin processing of this tissue. I may even add to soak the block a little more before taking the final sections. However, have you ever thought of processing into MMA resin? If you have these capabilities you may be very pleased with the results and find the microtomy less problematic. Let me know if or how I can be of assistance! Jack On May 15, 2010, at 4:30 AM, louise renton louise.ren...@gmail.com wrote: I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia reuel.corne...@tsrh.org wrote: How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] large fibrous bone tissue
I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia reuel.corne...@tsrh.orgwrote: How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] large fibrous bone tissue
How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
