RE: [Histonet] teeth sectioning
Thanks for your message Wayne. I will definitely follow up with you upon my return! Please let me know if there is anything else that interest you with regards to Hard Tissue specimen types. I specifically work with the histology related to bone, biomaterials and medical device implants. In fact, I will be presenting on these topics at several histology meetings here in the U.S. throughout the year: Indiana Society for Histotechnology - Indianapolis, IN (March 8-9) - Technological Advancements in Microtomy: A Non-Contact Alternative to Conventional Histology Equipment Techniques Georgia Society for Histotechnology - Jekyll Island, GA (April 12-13) - Laser Microtomy: The Future of Soft and Hard Tissue Histology LINK: http://www.histosearch.com/gsh/symposium.html Polysciences, Inc. Histological Applications and Techniques for Bone, Biomaterials and Medical Device Implants - Cambridge, MA (May 4) - Acrylic Resins: A Practical Approach for Demonstrating Bone, Biomaterials and Medical Device Implants LINK: http://www.polysciences.com/Interactive-Histology-Forum-Agenda/187/ Missouri Society for Histotechnology - Columbia, MO (May 30 - June 1) - Technological Advancements in Microtomy: A Non-Contact Alternative to Conventional Histology Equipment Techniques LINK: http://www.nsh.org/content/missouri-society-histotechnology-msh National Society for Histotechnology Symposium/Convention - Providence, RI (Sept 20-25) - A Detailed Examination of Working with Decalcified and Undecalcified Bone in Support of Preclinical and Clinical Research (co-presenter w/ Robert Skinner) LINK: http://www.histoconvention.org Hopefully I will get to meet you at one of these upcoming meetings! Best Regards, Jack Date: Mon, 4 Mar 2013 19:47:35 +0800 From: e...@pigsqq.org To: ratliffj...@hotmail.com CC: turke...@gmail.com; histonet@lists.utsouthwestern.edu; jratl...@ratliffhistology.com Subject: Re: Re: [Histonet] teeth sectioning Jack, That sounds really awesome. I did some work with the teeth of sows (female pigs) from specimens collected at slaughter. Those are very difficult to decalcify, and when finished, are likely to have no nuclear detail remaining. Interested to hear what you learn Wayne Johnson Beijing Enable Ag Consulting Yuanmingyuan West Road Meiyuan Com, On 3:59, Jack Ratliff wrote: Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekulturke...@gmail.com wrote: Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekulturke...@gmail.com wrote: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman
Re: Re: [Histonet] teeth sectioning
Jack, That sounds really awesome. I did some work with the teeth of sows (female pigs) from specimens collected at slaughter. Those are very difficult to decalcify, and when finished, are likely to have no nuclear detail remaining. Interested to hear what you learn Wayne Johnson Beijing Enable Ag Consulting Yuanmingyuan West Road Meiyuan Com, On 3:59, Jack Ratliff wrote: Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekulturke...@gmail.com wrote: Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekulturke...@gmail.com wrote: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. FNA Clia Guidelines (PicheGrocki, Jessica) 2. RE: FNA Clia Guidelines (Horn, Hazel V) 3. GSH Symposium April 12-14 (Zimmerman, Billie) 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) 5. (no subject) (Vikrant Piprode) 6. The GSH 4oth Anniversary Meeting (David Kemler) 7. QIHC (Renee H. Workman) -- Message: 1 Date: Thu, 28 Feb 2013 20:26:30 + From: PicheGrocki, Jessicajpiche-gro...@wtbyhosp.org Subject: [Histonet] FNA Clia Guidelines To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org Content-Type: text/plain; charset=s-ascii Hi All, Quick questionwhat are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital -- Message: 2 Date: Thu, 28
Re: [Histonet] teeth sectioning
Jack - I'm following your email up with an announcement once again about you and Dr. Heiko's interview on HistoTALK http://www.histotalk.com/. Maybe some of the folks out there would like to hear more about your involvment with laser histology. Have another great learning experience in Germany so that you can share it all with us upon your return. I'll pencil you in for another guest interview at the Georgia Society for Histotechnology's 40th Anniversary Meeting in April. If you haven't heard Jack and Dr. Hieko's interview about laser histology - it's a must listen! Yours, Dave Give Dr. my best! From: Jack Ratliff ratliffj...@hotmail.com To: mesruh turkekul turke...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; Jack Ratliff jratl...@ratliffhistology.com Sent: Friday, March 1, 2013 9:11 PM Subject: Re: [Histonet] teeth sectioning Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekul turke...@gmail.com wrote: Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul turke...@gmail.com wrote: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. FNA Clia Guidelines (PicheGrocki, Jessica) 2. RE: FNA Clia Guidelines (Horn, Hazel V) 3. GSH Symposium April 12-14 (Zimmerman, Billie) 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) 5. (no subject) (Vikrant Piprode) 6. The GSH 4oth Anniversary Meeting (David Kemler) 7. QIHC (Renee H. Workman) -- Message: 1 Date: Thu, 28 Feb 2013 20:26:30 + From: PicheGrocki, Jessica jpiche-gro...@wtbyhosp.org Subject: [Histonet] FNA Clia Guidelines To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org Content-Type: text/plain; charset=us-ascii Hi All, Quick questionwhat are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use
[Histonet] teeth sectioning
Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul turke...@gmail.com wrote: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. FNA Clia Guidelines (PicheGrocki, Jessica) 2. RE: FNA Clia Guidelines (Horn, Hazel V) 3. GSH Symposium April 12-14 (Zimmerman, Billie) 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) 5. (no subject) (Vikrant Piprode) 6. The GSH 4oth Anniversary Meeting (David Kemler) 7. QIHC (Renee H. Workman) -- Message: 1 Date: Thu, 28 Feb 2013 20:26:30 + From: PicheGrocki, Jessica jpiche-gro...@wtbyhosp.org Subject: [Histonet] FNA Clia Guidelines To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org Content-Type: text/plain; charset=us-ascii Hi All, Quick questionwhat are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital -- Message: 2 Date: Thu, 28 Feb 2013 15:05:35 -0600 From: Horn, Hazel V hor...@archildrens.org Subject: [Histonet] RE: FNA Clia Guidelines To: 'PicheGrocki, Jessica' jpiche-gro...@wtbyhosp.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 25a4de08332b19499904459f00aaacb719be135...@evs1.archildrens.org Content-Type: text/plain; charset=us-ascii Our pathologists do it all. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, Jessica Sent: Thursday, February 28, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA Clia Guidelines Hi All, Quick questionwhat are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c)
Re: [Histonet] teeth sectioning
Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekul turke...@gmail.com wrote: Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul turke...@gmail.com wrote: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. FNA Clia Guidelines (PicheGrocki, Jessica) 2. RE: FNA Clia Guidelines (Horn, Hazel V) 3. GSH Symposium April 12-14 (Zimmerman, Billie) 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) 5. (no subject) (Vikrant Piprode) 6. The GSH 4oth Anniversary Meeting (David Kemler) 7. QIHC (Renee H. Workman) -- Message: 1 Date: Thu, 28 Feb 2013 20:26:30 + From: PicheGrocki, Jessica jpiche-gro...@wtbyhosp.org Subject: [Histonet] FNA Clia Guidelines To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org Content-Type: text/plain; charset=us-ascii Hi All, Quick questionwhat are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital -- Message: 2 Date: Thu, 28 Feb 2013 15:05:35 -0600 From: Horn, Hazel V hor...@archildrens.org Subject: [Histonet] RE: FNA Clia Guidelines To: 'PicheGrocki, Jessica' jpiche-gro...@wtbyhosp.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID:
