[Histonet] Thanks! CD3 antibody for mouse IHC

[Connolly, Brett M via Histonet]

Thanks to all who replied to my CD3 query! The responses were just what I was 
hoping for.

Best regards,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks...also blog post

[Rene J Buesa via Histonet]

Long live the subterfuge!
René  

On Thursday, January 21, 2016 8:04 PM, Caroline Miller via Histonet 
 wrote:
 

 Thank you Lester, this is a great middle ground! As someone who was a
little put out by your totally off-topic emails, I am totally OK with you
promoting your blog post inside a legitimate histonet post.

Good luck with your issues.

yours,
mills

On Thu, Jan 21, 2016 at 12:03 PM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> http://www.chicagonow.com/downsize-maybe/2016/01/can-someone-create-this-app/
>
>
> Thanks to the suggestions for flooring. We are finding the concept of
> moving all our block and slide cabinets to allow us to lay new flooring
> overwhelming. We may opt to have the housekeeping crew put on a new coating
> that supposedly is more slip resistant. WE are also still researching our
> disposal of 10 year old blocks. Thanks for the suggestions.
>
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks...also blog post

[Lester Raff MD via Histonet]

http://www.chicagonow.com/downsize-maybe/2016/01/can-someone-create-this-app/


Thanks to the suggestions for flooring. We are finding the concept of moving 
all our block and slide cabinets to allow us to lay new flooring overwhelming. 
We may opt to have the housekeeping crew put on a new coating that supposedly 
is more slip resistant. WE are also still researching our disposal of 10 year 
old blocks. Thanks for the suggestions.


Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks...also blog post

[Caroline Miller via Histonet]

Thank you Lester, this is a great middle ground! As someone who was a
little put out by your totally off-topic emails, I am totally OK with you
promoting your blog post inside a legitimate histonet post.

Good luck with your issues.

yours,
mills

On Thu, Jan 21, 2016 at 12:03 PM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> http://www.chicagonow.com/downsize-maybe/2016/01/can-someone-create-this-app/
>
>
> Thanks to the suggestions for flooring. We are finding the concept of
> moving all our block and slide cabinets to allow us to lay new flooring
> overwhelming. We may opt to have the housekeeping crew put on a new coating
> that supposedly is more slip resistant. WE are also still researching our
> disposal of 10 year old blocks. Thanks for the suggestions.
>
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for assistance

[Lester Raff MD via Histonet]

Thanks for spreading the word about block disposal. I have been inundated with 
requests, will have to sort through them all!



For anyone interested Friday blog post 
http://www.chicagonow.com/downsize-maybe/2016/01/what-is-the-last-sound-you-will-hear/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!

[Cindy Bulmer]

To everyone who took the time to reply to my IHC survey,
Big help!!
 
Cindy
 

Cindy Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!

[Amy Lee]

Thanks to all who responded to my question regarding PAS staining!
We got much better staining after following you guys suggestion:
a. we purchased reagent from American Mastertech
b. we used 1% periodic acid
c. we rinse slides under warm water after Schiff's step.


Thanks again,

Amy
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] thanks

[Truscott, Tom]

Many thanks to all who contribute and keep the histonet running. I'm working my 
last day here at USDA-ARS ADRU within WSU which has been a very rewarding and 
enjoyable occupation. I was honored to be on 14 publications in 12 years with a 
few more in the works. Due to Federal budget constraints, they don't plan on 
replacing me with another BS An. Sci. ASCP HT. Happy New Year to you all! 
Thanks again Tom T= Thomas C. Truscott
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks! RE: thawing and refreezing muscle?

[Morken, Timothy]

Thanks to everyone for their muscle refreezing methods. We ended up being able 
to quickly split the samples and cut without thawing.  But we will try some out 
for the future, just in case.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center





-Original Message-
From: Hawkins, Hal K. [mailto:hhawk...@utmb.edu] 
Sent: Tuesday, October 01, 2013 1:35 PM
To: Walter Benton; Morken, Timothy; Histonet
Cc: jmitch...@neurology.wisc.edu
Subject: RE: thawing and refreezing muscle?


We have had reasonable results based on rapid thawing of frozen samples in room 
temperature buffer followed by formalin fixation or rapid freezing in OCT.  
With such large samples, though, there are bound to be large ice crystals in 
the interior of the specimen regardless of the method used for freezing. 


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Walter Benton 
[wben...@cua.md]
Sent: Tuesday, October 01, 2013 12:46 PM
To: Morken, Timothy; Histonet
Cc: jmitch...@neurology.wisc.edu
Subject: [Histonet] RE: thawing and refreezing muscle?

This may help. Never used it when I processed fresh/frozen muscle specimens but 
have heard good things.

http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf

http://www.lifebloodmedical.com/

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy 
[timothy.mor...@ucsfmedctr.org]
Sent: Tuesday, October 01, 2013 12:07 PM
To: Histonet
Cc: jmitch...@neurology.wisc.edu
Subject: [Histonet] thawing and refreezing muscle?

Histonetters, Does anyone have a good method for thawing muscle and refreezing 
for histochemistry? With good results? We have some bulk-frozen muscle 
(centimeter thick) and that is what they want to try.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
intended solely for the personal and confidential use of the designated 
recipient(s) named above and may contain information that is protected from 
disclosure under applicable law.  If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message without making a copy.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for your help on PA Grossing

[Wanda.Smith]

Thanks to ALL!
I have what I need!!!
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[Michael J. Lyon, Ph.D.]

I want to thank all of those that responded to my question so quickly.
Problem solved!!!  This is a great list with lots of helpful people.

 

Michael J. Lyon, PhD

Otolaryngology Research Labs

SUNY Upstate Medical University

750 East Adams Street

Syracuse, NY 13210

 

Voice: 315-464-7253

Fax: 315-464-5572

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] thanks

[Cynthia Pyse]

I want to thank everyone who responded to my chemical disposal question. I
now have adequate ammunition to combat my general manager's lack of
knowledge. He seems to think I don't know what I am talking about. Thanks
again for the backup it is appreciated. Have a great weekend.

Cindy

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory Manager

X-Cell Laboratories

20 Northpointe Parkway Suite 100

Amherst, NY 14228

716-250-9235 etx. 232

e-mail cp...@x-celllab.com

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histonet] thanks for your cooperation

[C.D.G.]

Rene J Buesa rjbu...@yahoo.com *** REPLY SEPARATOR ***

On 05/11/2012 at 09:44 a.m. Rene J Buesa wrote:
Estimado Carlos:
Adjunto está el trabajo de Fox et al y, como parece que te interesa el tema, te 
he adjuntado también dos trabajos míos sobre fijación con formol.
Saludos
René J.


From: C.D.G. late...@montevideo.com.uy
To: Histonet@lists.utsouthwestern.edu
Cc: Histonet@lists.utsouthwestern.edu
Sent: Sunday, November 4, 2012 8:23 PM
Subject: RE: AW: [Histonet] overfixation with formalin C.Fox pdf article





On 04/11/2012 at 07:01 p.m. joelle weaver wrote:

I will be pleased if someone could send to me a pdf copy of C.Fox article.
Thanks in advance,
Carlos Defeo
Histotechnologist
Montevideo,Uruguay




 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet


 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for dead fungi help

[Bartlett, Jeanine (CDC/OID/NCEZID)]

All:

Thanks for everyone's input.I have a plan!

Jeanine

-Original Message-
From: 
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: 11 October 2012 12:54
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] help with dead fungi


Good morning everyone,

Does anyone have  a protocol they use to demonstrate dead fungi in FFPE tissue?

Thanks!

Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
jeanine.bartl...@cdc.hhs.govmailto:jeanine.bartl...@cdc.hhs.gov

___
Histonet mailing list
Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
jeanine.bartl...@cdc.hhs.gov

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for all the good wishes

[Louise Renton]

AlsoI'll try to get the UNSUSCIBE UNSCRIBE UNSUSCRIBE  thingy
right when the time comes!

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
A good head and a good heart are always a formidable combination.
Nelson Mandelahttp://www.brainyquote.com/quotes/quotes/n/nelsonmand101682.html
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for the thoughts on Sequenza units!

[Keri Colwell]

Hello All,

I have compiled all of the responses to date regarding the question I posted on 
histonet about too much volume and reverse capillary action: (I've changed font 
styles to differentiate between responses)

I have used them for quite some time.  I was having problems with uneven 
staining and determined that it was from the being too full and was drawing up 
liquid from the bottom.  There is a small opening on the end and I just empty 
the solution from it.  I usually empty it after rinsing the antibody off and 
then usually before putting DAB or DAPI on depending on the number of slides 
stained.  As for washing, I just wash in a solution of soapy water and then 
rinse in distilled.  I use the coverplates for several staining times.  I love 
the sequenza and prefer it to having an automatic stainer.


I still use these and have 9 racks. 
The coverplates can be found on eBay, but I clean them in soapy bleach water, 
rinse well, and dry until the plastic gets brittle and they break.
I use AEC 99% of the time so I do not generate the waste of DAB.
If the racks are filing up with reagents and solutions, leave an empty slot and 
pipet it out. Otherwise, I dump it and and rinse after the run.
For the antibodies and detection solutions, you only need 3-4 drops. I only 
fill the well to the top with rinses.


We use these units, I have never had an issue with the waste reagents touching 
the bottom of the plates.  We never use more than 3 drops of kit items, the 
wells are filled with buffer.  It never seems to be alot of waste.
After we finish staining we just rinse the holders out with running water for a 
few minutes and let air dry.


We  empty them before they get to that stage, as this  would be  bound to 
affect the  efficient working of the Coverplates.


This is Tyler Liebig with the Thermo Scientific IHC group.  I'm responding to 
Keri's questions regarding the Sequenza manual staining.  Keri you are correct 
that if the waste is not emptied and builds up too much in the bottom of the 
sequenza unit it will definitely stop the capillary action (Reagent can't flow 
both ways :-).  However, this can be avoided by emptying the waste regularly 
between runs.  

You bring up a good question though that I didn't know the answer to: How many 
slides can be stained before the waste hits the slides? I did a quick 
calculation below that I hope is helpful.  

Total Waste Volume of Sequenza Rack:
In a Sequenza rack the slides are held about 1.5 inches above the bottom of the 
tray.  This allows about 270ml of waste before the level is close touching the 
slides.  

Reagent use per slide:  If you stain a single slide with a long protocol then 
worst case scenario is about 7 steps.  (H2O2 through Counterstain with a two 
step polymer)
Volume of rinse buffer: This means you would rinse 8 times 2ml each (16ml total 
rinse buffer per slide worst case).
For the staining reagents: The recommended volume is 0.1ml per reagent and 
0.5ml for chromogen.  So for 6 reagents plus chromogen that is 1.1ml and I will 
double it because I know some people add way more than needed.  So 2.2ml per 
slide worst case.

So to sum it up worst case scenario 18.2ml of waste is created per slide which 
means 14 slides (270/ml/18.2ml=14.8) can be stained before the waste risks 
touching the slides.  The rack holds ten slides total. 

In the end I recommend users play is save and empty the waste after each batch 
of slides stained even though most user generate much less waste than the 
calculations above.

I also included a link to a great description of Sequenza use on the Histonet 
posted by Gayle M Callis HTL/HT/MT (ASCP).  I have referenced these details 
frequently myself (Thanks Gayle).
http://www.histosearch.com/histonet/Jul02A/Longansweronusetechnicswi.html 


I have also read through Gayle's post on Histonet,  it is worth reading through 
for some more insight on the use of these units.



Keri








___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] THANKS for p16 info

[Settembre, Dana]

From: Amanda Kelley [mailto:akell...@slu.edu]
Sent: Tuesday, June 12, 2012 12:13 PM
To: Settembre, Dana
Cc: Vickroy, Jim; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] looking p16 - where is mtm labs?

You have to get current pricing on P16 from your local Ventana rep. It is 
available both in the vial and in the Ventana dispenser.
Amanda
On Tue, Jun 12, 2012 at 11:05 AM, Settembre, Dana 
sette...@umdnj.edumailto:sette...@umdnj.edu wrote:
Looking to get current pricing and availability from mtm labs on p16 and no one
answers their phone - 800# nor their direct#
Seems Roche has taken them over.
Need to reach them.
Any help would be appreciated
Dana Settembre
Immunohistochemistry
University Hospital - UMDNJ
Newark, NJ
USA

Histonet mailing list
Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



--
Amanda Kelley
Histology Supervisor
St. Louis University Medical School
Department of Pathology
1402 S. Grand Blvd.
St. Louis Mo. 63104
Phone: (314) 977-7868
Fax: (314) 977-8740
akell...@slu.edumailto:akell...@slu.edu

 DISCLAIMER:  The contents of this e-mail, including any attachments, contain 
information which may be confidential, legally privileged, proprietary in 
nature, or otherwise protected by law from disclosure, and is solely for the 
use of the intended recipient(s). If you are not the intended recipient, any 
use, disclosure or copying of this e-mail, including any attachments, is 
unauthorized and strictly prohibited. If you have received this e-mail in 
error, please notify us via return e-mail and immediately delete all copies of 
it from your system. Any opinions either expressed or implied in this e-mail 
and all attachments, are those of its author only, and do not necessarily 
reflect those of Saint Louis University.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] THANKS for p16 info

[Amanda Kelley]

You're Welcome!

On Tue, Jun 12, 2012 at 11:46 AM, Settembre, Dana sette...@umdnj.eduwrote:

 ** **

 ** **

 *From:* Amanda Kelley [mailto:akell...@slu.edu]
 *Sent:* Tuesday, June 12, 2012 12:13 PM
 *To:* Settembre, Dana
 *Cc:* Vickroy, Jim; histonet@lists.utsouthwestern.edu
 *Subject:* Re: [Histonet] looking p16 - where is mtm labs?

 ** **

 You have to get current pricing on P16 from your local Ventana rep. It is
 available both in the vial and in the Ventana dispenser.
 Amanda

 On Tue, Jun 12, 2012 at 11:05 AM, Settembre, Dana sette...@umdnj.edu
 wrote:

 Looking to get current pricing and availability from mtm labs on p16 and
 no one
 answers their phone - 800# nor their direct#
 Seems Roche has taken them over.
 Need to reach them.
 Any help would be appreciated
 Dana Settembre
 Immunohistochemistry
 University Hospital - UMDNJ
 Newark, NJ
 USA

 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




 --
 Amanda Kelley
 Histology Supervisor
 St. Louis University Medical School
 Department of Pathology
 1402 S. Grand Blvd.
 St. Louis Mo. 63104
 Phone: (314) 977-7868
 Fax: (314) 977-8740
 akell...@slu.edu

  DISCLAIMER:  The contents of this e-mail, including any attachments,
 contain information which may be confidential, legally privileged,
 proprietary in nature, or otherwise protected by law from disclosure, and
 is solely for the use of the intended recipient(s). If you are not the
 intended recipient, any use, disclosure or copying of this e-mail,
 including any attachments, is unauthorized and strictly prohibited. If you
 have received this e-mail in error, please notify us via return e-mail and
 immediately delete all copies of it from your system. Any opinions either
 expressed or implied in this e-mail and all attachments, are those of its
 author only, and do not necessarily reflect those of Saint Louis University.
 




-- 
Amanda Kelley
Histology Supervisor
St. Louis University Medical School
Department of Pathology
1402 S. Grand Blvd.
St. Louis Mo. 63104
Phone: (314) 977-7868
Fax: (314) 977-8740
akell...@slu.edu

 DISCLAIMER:  The contents of this e-mail, including any attachments,
contain information which may be confidential, legally privileged,
proprietary in nature, or otherwise protected by law from disclosure, and
is solely for the use of the intended recipient(s). If you are not the
intended recipient, any use, disclosure or copying of this e-mail,
including any attachments, is unauthorized and strictly prohibited. If you
have received this e-mail in error, please notify us via return e-mail and
immediately delete all copies of it from your system. Any opinions either
expressed or implied in this e-mail and all attachments, are those of its
author only, and do not necessarily reflect those of Saint Louis University.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks again

[Mayer,Toysha N]

I just wanted to say thanks again to all of those on histonet.  The questions 
that are asked go a long way in teaching troubleshooting.  Also, a few weeks 
ago the question was asked about purchasing forceps. I don't remember when or 
who.  One of the suggestions was jewelry forceps from craft stores such as 
Hobby Lobby.  That was a great suggestion!!  I took a field trip to Hobby Lobby 
and found some nice curved forceps with ergonomic finger pads for $3.99.  
Instead of ordering from Hobby Lobby, I called the distributor and was able to 
get 15 pairs for $2.99.  The tension and the weight of the forceps are good, 
and have a non-stick coating.  We will be placing them in our microtomy kits 
for next year.  For those interested, I also found Collins Scalpels (paraffin 
knives) at D R Instruments for $6.45 each if anyone needs some.


Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks again

[Jay Lundgren]

Texas go!  This is the kind of can do thinking that made our country so
great!

Imagine what the taxpayers could save if we multiplied that kind
of savings by every department in every large hospital in the US.

By the way, my father was a clinical Professor at MD Anderson, Lawrence E.
Lundgren, M.D.

 The eyes of Texas are upon you, all the livelong day.

Sincerely,

   Jay A. Lundgren,
M.S., HTL(ASCP)










___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks everyone!

[David Kemler]

Thank you all who joined me for the Region III Meeting at Callaway Gardens, 
Pine Mountain, GA hosted by the Georgia Society for Histotechnology this past 
weekend. The HistoTALK mobile studio always had someone stopping by to say 
hello. Lots of fun!
 
The interviews that were done at the Region III will begin airing this Sunday, 
April 22nd. So be sure to log on at www.HistoTALK.com. Our guest will be Jack 
Ratiff and it was a way cool interview you will agree. Somehow when Jack is on 
the show, it nearly becomes Wild Kingdom.
 
A special thanks goes out to Wanda Simons, Mike Ayers and the whole GSH  BOD 
for inviting me.
 
Yours,
David
 
BTW, HistoTALK was invited to be part of the Region VII Meeting in Phoenix, AZ 
on June 8 - 10. If you would like to be a guest on the show, just let me know 
and I'll plug you in.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[Marilyn Tyler]

Hi To All

A big thank you to everyone for their input regarding endogeous
peroxidase blocking.

Much appreciated

Marilyn



Medical School UCT
Dept of Surgery J50/26 or 30
Surgical research. OMB
Groote Schuur Hospital
Observatory
 
021-4066227
 




###

UNIVERSITY OF CAPE TOWN 

This e-mail is subject to the UCT ICT policies and e-mail disclaimer
published on our website at
http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from
+27 21 650 9111. This e-mail is intended only for the person(s) to whom
it is addressed. If the e-mail has reached you in error, please notify
the author. If you are not the intended recipient of the e-mail you may
not use, disclose, copy, redirect or print the content. If this e-mail
is not related to the business of UCT it is sent by the sender in the
sender's individual capacity.

###
 ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[Sheila Haas]

Thanks everyone. We'll start growing our own shortly. 
Thanks for the great ideas!
 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!

[D'Attilio, Shelley]

Hi all,
I wanted to thank all of you who responded to my question about my 
xylene-sensitive employee and purchase of an air filter system.  I got tons of 
terrific information and enjoyed the discussion about xylene substitutes.  
Those who responded gave me a lot to think about and raised some questions for 
me to have answered.

It's wonderful to have access to all of you experts on the listserv!  Thank you 
for your generosity.

Regards,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor 
accepting new patients.  Call (785) 354-5225.

**

The information transmitted in this e-mail and in any replies and forwards are 
for the sole use of the above individual(s) or entities and may contain 
proprietary, privileged and/or highly confidential information.  Any 
unauthorized dissemination, review, distribution or copying of these 
communications is strictly prohibited.  If this e-mail has been transmitted to 
you in error, please notify and return the original message to the sender 
immediately at the above listed address.  Thank you for your cooperation.

**

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks!

[Reuel Cornelia]

This was a good discussion and a some little rough comment  but we learn from 
it. I have read all the feedback on xylene and its substitute and it  gives us 
a lesson to become more better histotechs. Just this morning one of our 
administrator passed by near our processor while my co worker has just opened 
the processor retort for cleaning and smelled xylene in the air. He stopped and 
asked us why he was not using a mask while doing that. I replied that was only 
a minimal amount of xylene in the retort that was left after the cleaning cycle 
and he said well I just want to make sure of your safety. When we are so used 
of the smell sometimes we do not think of our Safety and this discussion taught 
us again to think first of our own Safety whether you are sensitive or not to 
xylene or any chemical.
To shelly, I wish your employee the best and all success in the field of 
histology.
 
 
Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768

 D'Attilio, Shelley sdatt...@stormontvail.org 9/28/2011 11:16 AM 
Hi all,
I wanted to thank all of you who responded to my question about my 
xylene-sensitive employee and purchase of an air filter system.  I got tons of 
terrific information and enjoyed the discussion about xylene substitutes.  
Those who responded gave me a lot to think about and raised some questions for 
me to have answered.

It's wonderful to have access to all of you experts on the listserv!  Thank you 
for your generosity.

Regards,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor 
accepting new patients.  Call (785) 354-5225.

**

The information transmitted in this e-mail and in any replies and forwards are 
for the sole use of the above individual(s) or entities and may contain 
proprietary, privileged and/or highly confidential information.  Any 
unauthorized dissemination, review, distribution or copying of these 
communications is strictly prohibited.  If this e-mail has been transmitted to 
you in error, please notify and return the original message to the sender 
immediately at the above listed address.  Thank you for your cooperation.

**

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[anuradha shrivastava]

Hello Histogurus,
Thank you very much for valuable advice.
Anu.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!

[Mary Helie]

Thank you all- this was very helpful. I appreciate it.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks to Leica serviceIn-Reply-To=

[Ruppert, Amysue]

One point to bring up, and I am sure that Leica service will do this, is the 
brand of knives you are using. Different brands have slightly different 
thickness. This is not related to the height of the blade. We have found that 
we need to use the blades that are used by our service company to calibrate the 
microtomes to have the correct tension in the holder and not any problems with 
the quality of the sections. 
 good luck, seems you are on the right track though.
amysue ruppert

__
The contents of this message may contain private, protected and/or privileged 
information.  If you received this message in error, you should destroy the 
e-mail message and any attachments or copies, and you are prohibited from 
retaining, distributing, disclosing or using any information contained within.  
Please contact the sender and advise of the erroneous delivery by return e-mail 
or telephone.  Thank you for your cooperation.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks to Leica service

[Scott]

Quick note for those who may be interested. I received a message from Leica's 
service department offering to help with my blade holder issues. Hopefully we 
can get the problem resolved quickly.

Thanks Leica !

Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for all of the Microtome feedback

[Scott Hendricksen]

Thanks for all of the Microtome feedback, it has been very helpful.  It sounds 
like everyone that has the RM 2235 loves it, and are not having a lot of 
problems.  Our Leica RM 2235 has problems with blade holder tension. We worked 
with Leica when it was just about 14 months old but they were no help. The 
problem is the front portion of the blade holder that clamps the blade in place 
is too thin or just not made of strong enough material. We don't have much 
tension on the blade while sectioning but the plate starts to curl or bend at 
the top where it meets the blade. Then the blade tension is inconsistent and 
the sectioning is inconsistent. We have been using a Leica 2030 blade holder 
for the last few years but its a little too short. Any suggestions on a blade 
holder  Has anyone had similar issues with their RM 2235  

Thanks !

Scott Hendricksen HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Thanks for all of the Microtome feedback

[Rathborne, Toni]

It must be out of warranty now, but why don't you price a new blade holder? Or 
better yet, ask Leica if they would replace the original one for you? If you do 
go to them, maybe you could request a different tech to evaluate it.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott 
Hendricksen
Sent: Saturday, July 30, 2011 3:15 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thanks for all of the Microtome feedback

Thanks for all of the Microtome feedback, it has been very helpful.  It sounds 
like everyone that has the RM 2235 loves it, and are not having a lot of 
problems.  Our Leica RM 2235 has problems with blade holder tension. We worked 
with Leica when it was just about 14 months old but they were no help. The 
problem is the front portion of the blade holder that clamps the blade in place 
is too thin or just not made of strong enough material. We don't have much 
tension on the blade while sectioning but the plate starts to curl or bend at 
the top where it meets the blade. Then the blade tension is inconsistent and 
the sectioning is inconsistent. We have been using a Leica 2030 blade holder 
for the last few years but its a little too short. Any suggestions on a blade 
holder  Has anyone had similar issues with their RM 2235  

Thanks !

Scott Hendricksen HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for Control Offers and Suggestions!!!

[Wanda.Smith]

Thanks to everyone for your offers and suggestions.  I am having a block sent 
from the control bank at NSH.
Thanks again to all,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax


This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for the hair processing information

[Martha Ward]

Thanks so much to the folks that responded to my request for information.  I 
forwarded them on to my colleague.


Martha Ward, MT (ASCP) QIHC
Assistant Manager
Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!

[Lindsey Minton]

Thanks Histonet and all of you that responded! I almost immediately got a 
response from a Thermo Fisher tech (Marganne you rock!) with the PDF.

Lindsey M. Minton
Jacksonville State University


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] thanks

[anuradha shrivastava]

Hi Histo Friends,
Thank you very much for yr help for PAS stain. you guys r great.
thanks again to every body.
Anu Shrivastava
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for all the leads

[Christine Tambasco]

Thanks for all the leads, please continue to send them as you hear about them. 
You guys are great! I will forward to my histo friend in need.

Christine Tambasco
Albany Medical Center
Albany,NY
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[Cynthia Pyse]

Happy Friday Histonetters

I want to thank everyone for the information on IHC strainers. This is just
what I needed. People who actually use the machines on a daily basis giving
their opinions. What a great forum we have. Hope everyone has a great
weekend. Currently we are receiving 6-10 inches of snow, hey, what do you
except in Buffalo. Thanks again.

Cindy

 

Cindy Pyse, CLT, HT(ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235

e-mail cp...@x-celllab.com

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks

[Mark Tarango]

So cruel!

On Fri, Feb 25, 2011 at 8:13 AM, sgoe...@mirnarx.com wrote:

 It's sunshine and 75 here in Texas...nanny nanny...I do love my state's
 weather!!  We had shorts on yesterday =)

 Sarah Goebel, BA, HT(ASCP)
 Histotechnologist
 Mirna Therapeutics
 2150 Woodward Street
 Suite 100
 Austin, Texas  78744
 (512)901-0900 ext. 6912


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
 Pyse
 Sent: Friday, February 25, 2011 9:38 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Thanks

 Happy Friday Histonetters

 I want to thank everyone for the information on IHC strainers. This is
 just
 what I needed. People who actually use the machines on a daily basis
 giving
 their opinions. What a great forum we have. Hope everyone has a great
 weekend. Currently we are receiving 6-10 inches of snow, hey, what do
 you
 except in Buffalo. Thanks again.

 Cindy



 Cindy Pyse, CLT, HT(ASCP)

 Laboratory/Histology Supervisor

 X-Cell Laboratories

 716-250-9235

 e-mail cp...@x-celllab.com





 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks

[Paula Pierce]

Texas Rocks! 2 years and 2 months until I'm home.
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com





From: sgoe...@mirnarx.com sgoe...@mirnarx.com
To: cp...@x-celllab.com; histonet@lists.utsouthwestern.edu
Sent: Fri, February 25, 2011 10:13:19 AM
Subject: RE: [Histonet] Thanks

It's sunshine and 75 here in Texas...nanny nanny...I do love my state's
weather!!  We had shorts on yesterday =)

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Pyse
Sent: Friday, February 25, 2011 9:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thanks

Happy Friday Histonetters

I want to thank everyone for the information on IHC strainers. This is
just
what I needed. People who actually use the machines on a daily basis
giving
their opinions. What a great forum we have. Hope everyone has a great
weekend. Currently we are receiving 6-10 inches of snow, hey, what do
you
except in Buffalo. Thanks again.

Cindy



Cindy Pyse, CLT, HT(ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235

e-mail cp...@x-celllab.com





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks so much!

[Kaye Ryan]

Thanks to everyone for their help with the mini fan pads!!

Kaye

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks interfacing the IHC bond and Cassettelabelers to Co-Path

[Akemi Allison]

Hi Histonet Land,

I just wanted to drop you a line and thank everyone that sent me  
direct and indirect information regarding my question interfacing  
the IHC bond and Cassettelabelers to Co-Path  I forwarded the  
information to the administrator that had proposed the question.  She  
was able to prose her information to the proper channels to make her  
case for justification.


Thank you again!

Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3...@yahoo.com

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[Sheila Haas]

Thanks to everyone for the information on xylene substitutes.
This should help me get the ball rolling.
 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks everyone!

[Kendall Neely]

Thank you to everyone who provided input on preferences for HE stainers last 
week. You were all very helpful!

Kendall A. Neely
Histology Technical Specialist
Shands Rocky Point Laboratories
(352) 265-0111, x72113


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3

[Connolly, Brett M]

Thanks to all for your suggestions for cutting cross sections of
cortical bone. I have passed them along.

Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray
Koelling's suggestion in the archives using an Ab from BD Biosciences.
Is it cat # 550295?

What others if any do you suggest? HEIR ? protocol tips?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com

 
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Thanks for bone cutting suggestions - and anti-Rat CD3

[Liz Chlipala]

Dako has a rabbit polyclonal that works in rat.  The other thing with
the bone, is place the slide with a section on a flat hotplate (60 C)
until it turns clear - not too long or the articular cartilage will
flip.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Tuesday, May 11, 2010 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat
CD3

Thanks to all for your suggestions for cutting cross sections of
cortical bone. I have passed them along.

Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray
Koelling's suggestion in the archives using an Ab from BD Biosciences.
Is it cat # 550295?

What others if any do you suggest? HEIR ? protocol tips?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com

 
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you
are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks

[Cynthia Pyse]

Thanks to all for providing me with the information I requested. You have
made my decision making so much easier. 

Thanks again

 

Cindy Pyse, CLT, HT (ASCP)

Histology Supervisor

X-Cell Laboratories

e-mail cp...@x-celllab.com

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks!!! Re: hematoxylin

[DELIA GARCIA]

Thank you all for your responses. I have tried a few things. But still going at 
it. :-) Have a great day!


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks to those who helped find Larry M. plus

[Leroy Brown]

I was able to speak to Larry M.  about  my tissue processor problems.  thank 
you all for your  help.  

I am now looking for perhaps a broken MVP-1 or 2 since I am no longer  able to 
get the CRT display parts to fix mine.   they are  interchangeable and if I 
could find a discarded MVP I could get mine running  again.   Anyone have a 
mothballed one sitting in the basement?  

Thanks  

LeRoy Brown HT(ASCP)HTL  

www.histocs.com  

Everson, Wa 98247
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for Response to Tonsil Controls Needed

[Henry, Charlene]

I want to thank all of you that have reached out and offered tonsil control to 
our laboratory. I have blocks that are now being sent to me and I think that I 
will will have plenty to last for a while. You guys are the greatest!!!
Again Thanks,

Charlene Henry HT (ASCP), QIHC
Anatomic Pathology Section Head
Department of Pathology
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105
901-595-3191
fax 901-595-3100



Email Disclaimer: www.stjude.org/emaildisclaimer
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for all the formalin waste responses

[Jessica Piche]

 
 
Hi All I just wanted to say thanks to everyone who has responded to my question 
regarding formalin waste going down the drain. I appreciate it! 
Have a great day!

Jessica Piche-Grocki, HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] thanks

[louise renton]

thanks to all who replied to my query regarding slide filing pages  - I have
lots of browsing to do today - yay!!!


have a great weekend!

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Thanks for capillary stain

[TF]

are there any useful histological techniques to stain capillary/blood vessel in 
brains?
and permit co-immunostaining of other cell markers?

PAS will stain all connective tissues, especially the myelin, thus you have to 
interpret your data well?


2008-12-27 



TF 



发件人： Nejat Yilmaz 
发送时间： 2008-12-27  04:44:34 
收件人： histonet@lists.utsouthwestern.edu 
抄送： 
主题： [Histonet] Thanks for capillary stain 
 
Dear All,
I'm very grateful for your kindly efforts. We've decided to stain our 
tissues with PAS. We couldn't perform IHC due to time limitations with the 
study. Because it usually takes about 2 months obtaining appropriate 
antibodies here. Fortunately I'm a member of this society.
I wish you a merry christmas and happy holidays...
Necat Yilmaz 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for all the help

[Marshall, Kimberly]

Hello again
 
  Want to say thanks for all the help with my haze  I have already tried 
filtering and it seems to have fixed the problem.  Just cuz they say no need to 
filter dont mean its true.  Again thanks for all the help
 
Kim

==
The information contained in this message may be privileged and/or confidential
and protected from disclosure.  If the reader of this message is not the 
intended 
recipient or an employee or agent responsible for delivering this message to 
the 
intended recipient, you are hereby notified that any dissemination, 
distribution 
or copying of this communication is strictly prohibited.  If you have received 
this
communication in error, please notify the sender immediately by replying to 
this 
message and deleting the material from any computer.

==
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] thanks Henwood for the PFA discussion

[Madary, Joseph]

At my previous job,I got so tired of the PFA discussion with a few folks
that I would hand them two containers of NBF one labeled PFA and one
labeled NBF and tell them to test it and they always went for the PFA.
I mean same stuff!  All in their heads man.  So those folks always got
the special labeled container.  Little placebo action never hurt eh?  I
notice that recycled formalin seems to boil off methanol too which is
what I use mainly- recycled.  Sometimes I have to add a little full
strength, but seems like it reads less alcohol after I run it thru the
recycler.  I see the billing point of methanol is 64.7 so it would
appear to be knocked off.

 

Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)

 

To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose.  Thank you for your cooperation.

 

 




To the extent this electronic communication or any of its attachments contain 
information that is not in the public domain, such information is considered by 
MedImmune to be confidential and proprietary.  This communication is expected 
to be read and/or used only by the individual(s) for whom it is intended.  If 
you have received this electronic communication in error, please reply to the 
sender advising of the error in transmission and delete the original message 
and any accompanying documents from your system immediately, without copying, 
reviewing or otherwise using them for any purpose.  Thank you for your 
cooperation.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks new labeler question

[Patti Loykasek]

Thanks to all for the answers on my expired reagents. Now, I have a new
question. Is anyone using the Brady label printer or another similar printer
for slide labels? I would be interested in hearing your experiences. Thanks,
as always. 

Patti Loykasek



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the 
intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks for help on desperately seeking help for cryosectioning and further question

[Barbara Schormair]

Dear All,

thank you so much for your suggestions on improving my cryosectioning 
results. I tried several of your suggestions, however, the quality of my 
sections did'nt improve so much.
I get perfect sections as long as I only cut the OCT embedding media. As 
soon as I reach embryonal tissue, only the tissue starts to tear up. In 
some of the embryos I loose almost all tissue. In my opinion this can 
only be caused by the fixation of the mouse embryos.

Here is our protocol:
1. Fixation in 4% PFA overnight at 4°C
2. 30% sucrose solution at 4°C till embryo sinks to bottom of the tube
3. transfer to 1:1 mix of 30% sucrose solution and OCT for 4h at 4°C
4. transfer to 100% OCT and freeze quickly in isopentane (cooled down 
with dry ice for approx. 30min before use).

5. store at -80°C

Is there something wrong? Should I follow a different protocol`?
How long can you store OCT-embedded tissue? Are 6 months too long?

Thanks so much for your help and best regards,

Barbara


--
Dipl. Biol. Barbara Schormair
PhD student 
Institute of Human Genetics

Tel.:   0049-89-3187-4097
Fax:0049-89-3187-3474
e-Mail: [EMAIL PROTECTED]

Helmholtz Zentrum München
German Research Center for Environmental Health (GmbH)
Ingolstaedter Landstraße 1
D-85764 Neuherberg
Germany


Chairman of Supervisory Board: MinDir Dr. Peter Lange
Board of Directors: Prof. Dr. Günther Wess and Dr. Nikolaus Blum
Register of Societies: Amtsgericht München HRB 6466 





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet