RE: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject
Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to processing. My techs say they cut like butter! Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, April 27, 2010 5:22 PM To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] toe nails falling off slides - Email found in subject
We have very good success in facing the block and soaking it on the water bath for 30-60 seconds and then allowing it to cool down on a very wet ice tray. Ours cut like butter too! :o) On Apr 28, 2010, at 8:58 AM, Cazares, Ruth rcaza...@schosp.org wrote: Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to processing. My techs say they cut like butter! Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, April 27, 2010 5:22 PM To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] toe nails falling off slides
To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] toe nails falling off slides
Using a plus slide, dip the slide into the water bath then add a small drop of Elmer's glue at one end and spread the glue with your finger along the length of the slide so that there is a thin film of glue. While the glue is still wet, add your sections and they should stay on through staining. Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Tuesday, April 27, 2010 3:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] toe nails falling off slides
HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] toe nails falling off slides
Can I ask, what exactly are you looking at toenails for? Is it a pathology thing or? That sounds so neat. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawrence Durrell, Clea On Tue, Apr 27, 2010 at 3:17 PM, zodia...@comcast.net wrote: To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
