Re: [Histonet] Unstained slides precut for IHC

[Tony Henwood (SCHN) via Histonet]

ation of heated detergent dewaxing and 
rehydration to immunohistochemistry. Biotechnic & Histochemistry, 87(1), 46-50.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Carrie Disbrow via Histonet 
Sent: Monday, 14 June 2021 12:04
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Unstained slides precut for IHC

Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven 
for 45 mins to dry.  If IHC is ordered a precut control and the already baked 
slides are again put in the 60 degree oven for 45 mins. Sometimes the control 
is cut and added to the dry slide or a separate slide.  So, the questions  have 
been should the   slides be air dried first using a fan before baking and 
slides not baked until the pathologist places an order? Should the tissue and 
the control all have the same amount of time in the oven to ensure consistency? 
Also, is it better to rack slides standing or on edge for IHC? Additionally, 
when sending slides for IHC to other labs is it preferred to send dry slides or 
baked slides? Thanks for your input!
Carrie Disbrow, HTL

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[Histonet] Unstained slides precut for IHC

[Carrie Disbrow via Histonet]

Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven 
for 45 mins to dry.  If IHC is ordered a precut control and the already baked 
slides are again put in the 60 degree oven for 45 mins. Sometimes the control 
is cut and added to the dry slide or a separate slide.  So, the questions  have 
been should the   slides be air dried first using a fan before baking and 
slides not baked until the pathologist places an order? Should the tissue and 
the control all have the same amount of time in the oven to ensure consistency? 
Also, is it better to rack slides standing or on edge for IHC? Additionally, 
when sending slides for IHC to other labs is it preferred to send dry slides or 
baked slides? Thanks for your input!
Carrie Disbrow, HTL

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Re: [Histonet] Unstained slides

[P Sicurello via Histonet]

How about frozen sections cut for immunofluorescence stored at -20?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Wed, Sep 5, 2018 at 1:12 PM Hobbs, Carl via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi
>
> Depends on what you mean by cryosections.
> Unfixed/fixed?
> Stored at RT, 4C, -20C, -80C.
> Stored dry or in glycerol
>
> So many variables!
> My opinion is to store blocks and cut sections as required.
> Least variables.
> Sure, one loses some tissue everytime one cuts anewa good thing.
>
> It IS complicated so, a project has to be thought out well in advance.
>
> Stimulating post
>
>
>
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge
> Kings College London
> London
> SE1 1UL
>
> 020 7848 6813
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Re: [Histonet] Unstained slides

[Hobbs, Carl via Histonet]

Hi

Depends on what you mean by cryosections.
Unfixed/fixed?
Stored at RT, 4C, -20C, -80C.
Stored dry or in glycerol

So many variables!
My opinion is to store blocks and cut sections as required.
Least variables.
Sure, one loses some tissue everytime one cuts anewa good thing.

It IS complicated so, a project has to be thought out well in advance.

Stimulating post


 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813
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Re: [Histonet] Unstained slides

[Ana Maluenda via Histonet]

Hi all,

It has been very interesting reading all your comments on unstained slides. 
This has been a forever discussion I always have everywhere I go in different 
research institutes. So expanding the topic, I wonder what's everyone's opinion 
on unstained cryosections? How long are they reliable to be used for IHC?

Kind regards,

Ana

Ana Maluenda
Research Assistant
Atherothrombosis and Vascular Biology Laboratory

Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne VIC 3004
P (03) 8532 1359 E ana.malue...@baker.edu.au W www.baker.edu.au


-Original Message-
From: Hobbs, Carl [mailto:carl.ho...@kcl.ac.uk]
Sent: Tuesday, 4 September 2018 4:54 AM
To: histonet 
Subject: Re: [Histonet] Unstained slides

I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because, if this "oxidation" theory is true, then the 
initial sections will be no good.
However, careful organisation of exptl procedure before actual cutting will 
work very well.

Actually, not many Ags get "oxidised"for eg: I can demonstrate GFAP in 
sections that are a year old ( sure, they are stored at 4C just in case) These 
slides are used for Yr 1 BSc practicals and are consistently positive.
Nobody knows why some Ags ( and not others) lose their antigenicity, imho 
Oxidation is a vague reasoning.
Just like nobody really knows why HIER works: however, I am in the dipole 
moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed 
specimens.

Curious-illy

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813

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Re: [Histonet] Unstained slides

[Hobbs, Carl via Histonet]

I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because, if this "oxidation" theory is true, then the 
initial sections will be no good.
However, careful organisation of exptl procedure before actual cutting will 
work very well.

Actually, not many Ags get "oxidised"for eg: I can demonstrate GFAP in 
sections that are a year old ( sure, they are stored at 4C just in case)
These slides are used for Yr 1 BSc practicals and are consistently positive.
Nobody knows why some Ags ( and not others) lose their antigenicity, imho
Oxidation is a vague reasoning.
Just like nobody really knows why HIER works: however, I am in the dipole 
moment school of thought, rather than the Ca++ skool
Sure, in Formalin-fixed specimens.

Curious-illy

Carl  
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813
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Re: [Histonet] Unstained slides

[Rene J Buesa via Histonet]

On this issue of lost of antigenicity, never forget air oxygen!René 

On Monday, September 3, 2018 11:11 AM, "Frazier, John" 
 wrote:
 

 Interesting that you stated that, I was at the university of Colorado
this past week and was speaking with the medical director of the
pathology department. We actually started talking about unstained
slides and their storage conditions. We actually spoke of the histonet
discussions around unstained slide storage.  He stated to me that due
to the elevation and lack of humidity in Denver that the antigenicity
of unstained slides has been up to multiple years. This is due to, as
you stated, water in the tissue.

Sent from my iPad

> On Sep 3, 2018, at 9:42 AM, Cartun, Richard  
> wrote:
>
> It appears that the presence of water, both endogenously and exogenously, 
> plays a central role in the loss of antigenicity in stored unstained slides 
> (see reference below).  Labs that are experiencing significant loss of 
> immunoreactivity in their unstained slides should check their tissue 
> processing.
>
> Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation of 
> archival formalin-fixed, paraffin-embedded tissue sections.  J of Histochem 
> Cytochem 2011; 59:356-365.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links 
> from unknown senders.
>
> Everything has been pointed out is correct BUT also pivot on "how the 
> unstained slides are kept".Kept in a box their "useful life" is quite short 
> (not beyond 1 week at the most).Kept at -80ºC I have used them after years of 
> being stored the principle being of deep-freezing and this is "standard 
> procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil 
> or paraffin covered I have used them after months of being stored the 
> principle being that, isolated from air oxygen, epitopes do not oxidize 
> ("weaken") of if they do, the rate is greatly slowed.On the other hand, 
> usually, unstained slides are kept for only few days in the event that, lets 
> say within a week, the PT decides to order some special procedure and 
> sometimes it is impossible "return" to the original block many times "almost 
> exhausted".Properly done storing unstained slides are extremely useful.René
>
>    On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" 
> wrote:
>
>
> I agree with Tim as well. This is what we advise our clients to do. It takes 
> some coordination with the pathologist, but it is the best strategy for 
> reducing unnecessary unstained slides. In the studies that we have performed 
> only 10% of the unstained slides that are cut are you and the 90% are are it 
> takes some coordination with the pathologist, but it is the best strategy for 
> reducing unnecessary unstained slides. In the studies that we have performed 
> only 10% of the unstained slides that are cut are you and 90% are thrown away 
> thrown away.
> Several laboratories that I have visited in order to reduce the amount of 
> wasted tissue when refacing the blocks, is to reseal the blocks with liquid 
> paraffin, that have scant or small amounts of tissue in the block, such as 
> the needle core biopsy.
> Bottom line on this issue is to educate the pathologist, and not water and 
> stain slides except in rare occasions
>
> Sent from my iPhone
>
>> On Aug 17, 2018, at 14:07, Terri Braud  wrote:
>>
>> I'm with Tim Morken on this one. The variability of antigenicity in storage 
>> is so wide open, and there really is no recent data, so we just make a point 
>> of educating our techs on not wasting tissue/levels during sectioning.  If 
>> the techs feel that the residual tissue in the block is in danger of being 
>> exhausted, we communicate with our pathologists on how best to handle any 
>> requests.  Unstained slides was time, money, and storage and we are better 
>> off without them.
>>
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingd

Re: [Histonet] Unstained slides

[Frazier, John via Histonet]

Interesting that you stated that, I was at the university of Colorado
this past week and was speaking with the medical director of the
pathology department. We actually started talking about unstained
slides and their storage conditions. We actually spoke of the histonet
discussions around unstained slide storage.  He stated to me that due
to the elevation and lack of humidity in Denver that the antigenicity
of unstained slides has been up to multiple years. This is due to, as
you stated, water in the tissue.

Sent from my iPad

> On Sep 3, 2018, at 9:42 AM, Cartun, Richard  
> wrote:
>
> It appears that the presence of water, both endogenously and exogenously, 
> plays a central role in the loss of antigenicity in stored unstained slides 
> (see reference below).  Labs that are experiencing significant loss of 
> immunoreactivity in their unstained slides should check their tissue 
> processing.
>
> Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation of 
> archival formalin-fixed, paraffin-embedded tissue sections.  J of Histochem 
> Cytochem 2011; 59:356-365.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links 
> from unknown senders.
>
> Everything has been pointed out is correct BUT also pivot on "how the 
> unstained slides are kept".Kept in a box their "useful life" is quite short 
> (not beyond 1 week at the most).Kept at -80ºC I have used them after years of 
> being stored the principle being of deep-freezing and this is "standard 
> procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil 
> or paraffin covered I have used them after months of being stored the 
> principle being that, isolated from air oxygen, epitopes do not oxidize 
> ("weaken") of if they do, the rate is greatly slowed.On the other hand, 
> usually, unstained slides are kept for only few days in the event that, lets 
> say within a week, the PT decides to order some special procedure and 
> sometimes it is impossible "return" to the original block many times "almost 
> exhausted".Properly done storing unstained slides are extremely useful.René
>
>On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" 
>  wrote:
>
>
> I agree with Tim as well. This is what we advise our clients to do. It takes 
> some coordination with the pathologist, but it is the best strategy for 
> reducing unnecessary unstained slides. In the studies that we have performed 
> only 10% of the unstained slides that are cut are you and the 90% are are it 
> takes some coordination with the pathologist, but it is the best strategy for 
> reducing unnecessary unstained slides. In the studies that we have performed 
> only 10% of the unstained slides that are cut are you and 90% are thrown away 
> thrown away.
> Several laboratories that I have visited in order to reduce the amount of 
> wasted tissue when refacing the blocks, is to reseal the blocks with liquid 
> paraffin, that have scant or small amounts of tissue in the block, such as 
> the needle core biopsy.
> Bottom line on this issue is to educate the pathologist, and not water and 
> stain slides except in rare occasions
>
> Sent from my iPhone
>
>> On Aug 17, 2018, at 14:07, Terri Braud  wrote:
>>
>> I'm with Tim Morken on this one. The variability of antigenicity in storage 
>> is so wide open, and there really is no recent data, so we just make a point 
>> of educating our techs on not wasting tissue/levels during sectioning.  If 
>> the techs feel that the residual tissue in the block is in danger of being 
>> exhausted, we communicate with our pathologists on how best to handle any 
>> requests.  Unstained slides was time, money, and storage and we are better 
>> off without them.
>>
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>>
>> Today'

Re: [Histonet] Unstained slides

[Cartun, Richard via Histonet]

It appears that the presence of water, both endogenously and exogenously, plays 
a central role in the loss of antigenicity in stored unstained slides (see 
reference below).  Labs that are experiencing significant loss of 
immunoreactivity in their unstained slides should check their tissue processing.

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation of 
archival formalin-fixed, paraffin-embedded tissue sections.  J of Histochem 
Cytochem 2011; 59:356-365.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Sunday, August 19, 2018 2:09 PM
To: Frazier, John; Terri Braud
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides

This is an email from Outside HHC. USE CAUTION opening attachments or links 
from unknown senders.

Everything has been pointed out is correct BUT also pivot on "how the unstained 
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 
week at the most).Kept at -80ºC I have used them after years of being stored 
the principle being of deep-freezing and this is "standard procedure" for IDF 
"+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I 
have used them after months of being stored the principle being that, isolated 
from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is 
greatly slowed.On the other hand, usually, unstained slides are kept for only 
few days in the event that, lets say within a week, the PT decides to order 
some special procedure and sometimes it is impossible "return" to the original 
block many times "almost exhausted".Properly done storing unstained slides are 
extremely useful.René

On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" 
 wrote:


 I agree with Tim as well. This is what we advise our clients to do. It takes 
some coordination with the pathologist, but it is the best strategy for 
reducing unnecessary unstained slides. In the studies that we have performed 
only 10% of the unstained slides that are cut are you and the 90% are are it 
takes some coordination with the pathologist, but it is the best strategy for 
reducing unnecessary unstained slides. In the studies that we have performed 
only 10% of the unstained slides that are cut are you and 90% are thrown away 
thrown away.
Several laboratories that I have visited in order to reduce the amount of 
wasted tissue when refacing the blocks, is to reseal the blocks with liquid 
paraffin, that have scant or small amounts of tissue in the block, such as the 
needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water and 
stain slides except in rare occasions

Sent from my iPhone

> On Aug 17, 2018, at 14:07, Terri Braud  wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage 
> is so wide open, and there really is no recent data, so we just make a point 
> of educating our techs on not wasting tissue/levels during sectioning.  If 
> the techs feel that the residual tissue in the block is in danger of being 
> exhausted, we communicate with our pathologists on how best to handle any 
> requests.  Unstained slides was time, money, and storage and we are better 
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
>  7. Re: Unstained slides - how long are they good for?
>      (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy" 
> To: P Sicurello 
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had 
> kept them indefinitely, then when storage was overwhelming us we reduced it 
> to 2 months maximum. Now we require request for unstained to be ordered in 
> the system and delivered to the pathologist. We do not hold any in the lab. 
> We recut when new stains are ordered. In the past we had routinely cut extras 
> "just in case" but ended up with thousands of unstained slides that were 
> never used. Instead we trained everyone to reduce wastage and get good 
> sections from a cut block with minimal facing. We have not stored unstained 
> sections for many years and the

[Histonet] Unstained Slides - results

[P Sicurello via Histonet]

Good Afternoon Listers,

Thanks to all who answered my question about retention of unstained
slides.  I had several people weigh in on the topic and we have created an
unstained slide policy based on their advice and the literature that was
kindly sent to me.

The consensus is antigenicity degrades depending on storage conditions,
among other things, which can lead to false negative results (antibody
dependent - but not worth the risk).  We now will keep unstained slides
which have been stored at room temperature no longer than 12 weeks.  One
really good suggestion that will are implementing is if a pathologist wants
the slides kept longer than 12 weeks, they get to keep the slides in their
possession.  We will enter a note in our LIS stating who has the unstained
slides.

The wisdom of my electronic colleagues comes through once again.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
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retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
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Re: [Histonet] Unstained slides - how long are they good for?

[Mark Tarango via Histonet]

Hi Everyone!

I have seen unstained slides save a patient from re-biopsy many times.
Usually it will be a case where a patient has a known diagnosis, like lung
cancer.  In these types of cases after diagnosis molecular testing (and IHC
for PD-L1) is usually ordered.  There have been countless times that I can
recall where a few unstained slides on a biopsy with scant tumor was able
to get us results for PD-L1, ALK FISH, and ROS1 FISH.  Often in these types
of a cases a touch prep can be used for Next Generation Sequencing or PCR
testing like EGFR or BRAF, allowing for the full panel of molecular tests
to be performed.  For cases that are small specimens I would prefer to have
unstained slides to fall back on for patient convenience, client
satisfaction, and quicker TAT of molecular testing.

Re-biopsy and re-diagnosing the new sample costs money to the patient and
payers and having some unstained slides can often save those costs
providing more value to the original biopsy.  Sometimes when we try to save
money in the lab it can result in more money being spent on healthcare
overall. It is true that some antigens become more difficult to stain over
time and storage is an important consideration.  Limiting the production of
unstained slides to small and scant needle may make storage more practical.

Just some more things to consider.

Sincerely,

Mark Tarango


On Thu, Aug 16, 2018 at 4:48 PM, P Sicurello via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up..  How long are *unstained* slides good for?
> Not for H but tests like IHC and molecular testing.  These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material.  Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited.  If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
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Re: [Histonet] Unstained slides - how long are they good for?

[Jamie Watson via Histonet]

To expand on what we do at our research lab; we use 6 months as a standard 
maximum age of unstained slides.  Also in the staining protocol for each 
antibodyl we have a specific shelf life for the diluted Antibody and a 
maximum age of unstained slides.

Jamie



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Re: [Histonet] Unstained slides - how long are they good for?

[Hobbs, Carl via Histonet]

I agree with Jamie

Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let 
it be YOUR protein of interest.
If you really are concerned, cut fresh sections and immunostain along with your 
stored sections.

Imho: cut as few sections as you need.
Store any unused at 4C

Best wishes
 
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813
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Re: [Histonet] Unstained slides

[Rene J Buesa via Histonet]

Everything has been pointed out is correct BUT also pivot on "how the unstained 
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 
week at the most).Kept at -80ºC I have used them after years of being stored 
the principle being of deep-freezing and this is "standard procedure" for IDF 
"+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I 
have used them after months of being stored the principle being that, isolated 
from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is 
greatly slowed.On the other hand, usually, unstained slides are kept for only 
few days in the event that, lets say within a week, the PT decides to order 
some special procedure and sometimes it is impossible "return" to the original 
block many times "almost exhausted".Properly done storing unstained slides are 
extremely useful.René 

On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" 
 wrote:
 

 I agree with Tim as well. This is what we advise our clients to do. It
takes some coordination with the pathologist, but it is the best
strategy for reducing unnecessary unstained slides. In the studies
that we have performed only 10% of the unstained slides that are cut
are you and the 90% are are it takes some coordination with the
pathologist, but it is the best strategy for reducing unnecessary
unstained slides. In the studies that we have performed only 10% of
the unstained slides that are cut are you and 90% are thrown away
thrown away.
Several laboratories that I have visited in order to reduce the amount
of wasted tissue when refacing the blocks, is to reseal the blocks
with liquid paraffin, that have scant or small amounts of tissue in
the block, such as the needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water
and stain slides except in rare occasions

Sent from my iPhone

> On Aug 17, 2018, at 14:07, Terri Braud  wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage 
> is so wide open, and there really is no recent data, so we just make a point 
> of educating our techs on not wasting tissue/levels during sectioning.  If 
> the techs feel that the residual tissue in the block is in danger of being 
> exhausted, we communicate with our pathologists on how best to handle any 
> requests.  Unstained slides was time, money, and storage and we are better 
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
>  7. Re: Unstained slides - how long are they good for?
>      (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy" 
> To: P Sicurello 
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had 
> kept them indefinitely, then when storage was overwhelming us we reduced it 
> to 2 months maximum. Now we require request for unstained to be ordered in 
> the system and delivered to the pathologist. We do not hold any in the lab. 
> We recut when new stains are ordered. In the past we had routinely cut extras 
> "just in case" but ended up with thousands of unstained slides that were 
> never used. Instead we trained everyone to reduce wastage and get good 
> sections from a cut block with minimal facing. We have not stored unstained 
> sections for many years and they do not seem to be missed.
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -Original Message-
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, August 16, 2018 4:49 PM
> To: HistoNet
> Subject: [Histonet] Unstained slides - how long are they good for?
>
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up..  How long are *unstained* slides good for?
> Not for H but tests like IHC and molecular testing.  These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
&

Re: [Histonet] Unstained slides

[Frazier, John via Histonet]

I agree with Tim as well. This is what we advise our clients to do. It
takes some coordination with the pathologist, but it is the best
strategy for reducing unnecessary unstained slides. In the studies
that we have performed only 10% of the unstained slides that are cut
are you and the 90% are are it takes some coordination with the
pathologist, but it is the best strategy for reducing unnecessary
unstained slides. In the studies that we have performed only 10% of
the unstained slides that are cut are you and 90% are thrown away
thrown away.
Several laboratories that I have visited in order to reduce the amount
of wasted tissue when refacing the blocks, is to reseal the blocks
with liquid paraffin, that have scant or small amounts of tissue in
the block, such as the needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water
and stain slides except in rare occasions

Sent from my iPhone

> On Aug 17, 2018, at 14:07, Terri Braud  wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage 
> is so wide open, and there really is no recent data, so we just make a point 
> of educating our techs on not wasting tissue/levels during sectioning.  If 
> the techs feel that the residual tissue in the block is in danger of being 
> exhausted, we communicate with our pathologists on how best to handle any 
> requests.  Unstained slides was time, money, and storage and we are better 
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
>   7. Re: Unstained slides - how long are they good for?
>  (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy" 
> To: P Sicurello 
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had 
> kept them indefinitely, then when storage was overwhelming us we reduced it 
> to 2 months maximum. Now we require request for unstained to be ordered in 
> the system and delivered to the pathologist. We do not hold any in the lab. 
> We recut when new stains are ordered. In the past we had routinely cut extras 
> "just in case" but ended up with thousands of unstained slides that were 
> never used. Instead we trained everyone to reduce wastage and get good 
> sections from a cut block with minimal facing. We have not stored unstained 
> sections for many years and they do not seem to be missed.
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -Original Message-----
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, August 16, 2018 4:49 PM
> To: HistoNet
> Subject: [Histonet] Unstained slides - how long are they good for?
>
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up..  How long are *unstained* slides good for?
> Not for H but tests like IHC and molecular testing.  These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material.  Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited.  If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> --
>
> Subject: Digest Footer
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>
> --
>
> End of Histonet Digest, Vol 177, Issue 16
> *
>
>
>

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Re: [Histonet] Unstained slides - how long are they good for?

[Frazier, John via Histonet]

I’m a histology workflow consultant that visits many AP laboratories
each year. Almost every laboratory has a different retention policy.
The average of most laboratories is to hold onto unstained slides for
three weeks after final sign out. Typically the unstained slide can be
held for a long period of time if used just for morphological
staining. However if the unstained slides is going to be used for IHC
or molecular testing, the antigenicity of the slide begins degrading
at the point of cutting. Typically, however, for a high-quality IHC
staining, if stored at room temperature, the unstained slide should
not be held much longer than one month. And even at that time frame
you will begin to see the degrading of the stain quality. If the
slides are kept in a closed box, in refrigerator, they have longer
retention. Typically up to 2 to 3 months.
I hope this helps

Sent from my iPhone

> On Aug 16, 2018, at 19:48, P Sicurello  wrote:
>
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up..  How long are *unstained* slides good for?
> Not for H but tests like IHC and molecular testing.  These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material.  Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited.  If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
>

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Re: [Histonet] Unstained slides

[Terri Braud via Histonet]

I'm with Tim Morken on this one. The variability of antigenicity in storage is 
so wide open, and there really is no recent data, so we just make a point of 
educating our techs on not wasting tissue/levels during sectioning.  If the 
techs feel that the residual tissue in the block is in danger of being 
exhausted, we communicate with our pathologists on how best to handle any 
requests.  Unstained slides was time, money, and storage and we are better off 
without them.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:
   7. Re: Unstained slides - how long are they good for?
  (Morken, Timothy)

Message: 7
Date: Fri, 17 Aug 2018 15:16:00 +
From: "Morken, Timothy" 
To: P Sicurello 
Subject: Re: [Histonet] Unstained slides - how long are they good for?


Paula, since it is variable we strive to not have unstained slides. We had kept 
them indefinitely, then when storage was overwhelming us we reduced it to 2 
months maximum. Now we require request for unstained to be ordered in the 
system and delivered to the pathologist. We do not hold any in the lab. We 
recut when new stains are ordered. In the past we had routinely cut extras 
"just in case" but ended up with thousands of unstained slides that were never 
used. Instead we trained everyone to reduce wastage and get good sections from 
a cut block with minimal facing. We have not stored unstained sections for many 
years and they do not seem to be missed. 

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, August 16, 2018 4:49 PM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up..  How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing.  These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.

Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
___
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--

Subject: Digest Footer

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End of Histonet Digest, Vol 177, Issue 16
*


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Re: [Histonet] Unstained slides - how long are they good for?

[Morken, Timothy via Histonet]

Paula, since it is variable we strive to not have unstained slides. We had kept 
them indefinitely, then when storage was overwhelming us we reduced it to 2 
months maximum. Now we require request for unstained to be ordered in the 
system and delivered to the pathologist. We do not hold any in the lab. We 
recut when new stains are ordered. In the past we had routinely cut extras 
"just in case" but ended up with thousands of unstained slides that were never 
used. Instead we trained everyone to reduce wastage and get good sections from 
a cut block with minimal facing. We have not stored unstained sections for many 
years and they do not seem to be missed. 

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, August 16, 2018 4:49 PM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up..  How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing.  These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.

Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] Unstained slides - how long are they good for?

[Tony Henwood (SCHN) via Histonet]

Will definitely depend on the antibody you are using. Some references:

Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss 
of tumor marker-immunostaining intensity on stored paraffin slides of breast 
cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059.

Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of 
tumor marker-immunostaining intensity on stored paraffin slides of breast 
cancer. Journal of the National Cancer Institute, 89(8), 585-586.

Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., Vérola, O., 
Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical 
reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 
370-374.

Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of 
immunohistochemical reactivity on stored paraffin slides. Journal of clinical 
pathology, 51(12), 943.

Wester, K., Wahlund, E., Sundström, C., Ranefall, P., Bengtsson, E., Russell, 
P. J., ... & Busch, C. (2000). Paraffin section storage and 
immunohistochemistry: effects of time, temperature, fixation, and retrieval 
protocol with emphasis on p53 protein and MIB1 antigen. Applied 
Immunohistochemistry & Molecular Morphology, 8(1), 61-70.

van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in 
diagnostic and research immunohistochemistry associated with epitope 
instability in stored paraffin sections. Applied Immunohistochemistry & 
Molecular Morphology, 8(4), 316-321. 

Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, 
T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of 
storage-related alterations in immunoreactivity of archival tissue sections and 
its implications for collaborative multicenter immunohistochemical studies. 
Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266.

Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., Dürmüller, U., Simon, R., ... 
& Sauter, G. (2004). Influence of slide aging on results of translational 
research studies using immunohistochemistry. Modern pathology, 17(11), 1414.

DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term 
preservation of antigenicity on tissue microarrays. Laboratory investigation, 
84(8), 1071.

Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. 
C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast 
cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 
13(4), 667-672.

Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of 
prostate needle biopsies using saved interval sections vs new recut sections 
from the block: a prospective comparison. American journal of clinical 
pathology, 131(5), 683-688.

Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., 
... & Hewitt, S. M. (2011). Factors influencing the degradation of archival 
formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & 
Cytochemistry, 59(4), 356-365.

Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, 
E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately 
stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64.

Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & 
Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded 
tissues on the in situ hybridization and immunohistochemistry signals in 
cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173.

Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., 
& Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: 
is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017.

Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., 
Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 
Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological 
Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494.


-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, 17 August 2018 9:49 AM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up..  How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing.  These slides have been 
cut, stored at room temperature, not sealed in anyway, and kept in a cardboard 
box.

Please let me know what your opinions are and what your retention policy is 
concerning *unstained* slides.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

Re: [Histonet] Unstained slides - how long are they good for?

[Jamie Watson via Histonet]

It depends on the stability of the protein and fixation, some are stable 
for a week some for years. We use 6 months as a standard.

Jamie

On August 16, 2018 4:59:34 PM P Sicurello via Histonet 
 wrote:

> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up..  How long are *unstained* slides good for?
> Not for H but tests like IHC and molecular testing.  These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material.  Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited.  If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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[Histonet] Unstained slides - how long are they good for?

[P Sicurello via Histonet]

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up..  How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing.  These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.

Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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RE: [Histonet] Unstained slides

[Tom McNemar]

We cut a decent number of unstained as well.  Mostly the tiny biopsies and 
cores and we do end up using most of these. I personally see no reason to cut 
unstained slides on anything else.  Since you still have to go back and cut 
controls, it doesn't really save that much time but of course, it does save 
tissue.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Wednesday, August 28, 2013 4:47 PM
To: Martha Ward-Pathology
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides

Martha,

I have had experience in three different labs.  The unstained slides can
pile up quickly, especially when you are using charged slides that are
just in case slides for sendouts, IHC, etc.  For core biopsies (breasts,
fine needles, prostate, etc.) we cut levels and put up at least five of the
in between sections to keep on hand in case.  We maybe use these slides
10 percent of the time and toss the rest after 3-4 weeks.  It can seem
wasteful, however if you have a small core, you must save precious tissue.

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Wed, Aug 28, 2013 at 9:28 AM, Martha Ward-Pathology mw...@wakehealth.edu
 wrote:



 We are looking at ways to improve our work processes, save time and labor
 and reduce costs, all while maintaining patient quality...as we all are of
 course.

 During our conversations the subject of cutting unstained slides has come
 up and we are looking for bench marking data to see if we are where we need
 to be.Currently we are cutting unstained slides for various protocols
 (including prostate biopsies), where the specimens are tiny,  but we are
 also cutting a lot of just in case unstained slides.   Our research has
 shown that about 50% of the time the unstained slides requested are never
 used and we are trying to find out if that is high, low or about what other
 institutions are seeing.   If anyone has any data they could share with us
 we would appreciate it.

 Thanks in advance for your help.   I can share what I find out if others
 are interested.


 Martha Ward, MT (ASCP) QIHC
 Manager

 Molecular Diagnostics Lab
 Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f
 336.716.5890 mw...@wakehealth.edu





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[Histonet] Unstained slides

[Martha Ward-Pathology]

We are looking at ways to improve our work processes, save time and labor and 
reduce costs, all while maintaining patient quality...as we all are of course.

During our conversations the subject of cutting unstained slides has come up 
and we are looking for bench marking data to see if we are where we need to be. 
   Currently we are cutting unstained slides for various protocols (including 
prostate biopsies), where the specimens are tiny,  but we are also cutting a 
lot of just in case unstained slides.   Our research has shown that about 50% 
of the time the unstained slides requested are never used and we are trying to 
find out if that is high, low or about what other institutions are seeing.   If 
anyone has any data they could share with us we would appreciate it.   

Thanks in advance for your help.   I can share what I find out if others are 
interested.


Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 
336.716.5890 mw...@wakehealth.edu  





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Re: [Histonet] Unstained slides

[Beth Brinegar]

Martha,

I have had experience in three different labs.  The unstained slides can
pile up quickly, especially when you are using charged slides that are
just in case slides for sendouts, IHC, etc.  For core biopsies (breasts,
fine needles, prostate, etc.) we cut levels and put up at least five of the
in between sections to keep on hand in case.  We maybe use these slides
10 percent of the time and toss the rest after 3-4 weeks.  It can seem
wasteful, however if you have a small core, you must save precious tissue.

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Wed, Aug 28, 2013 at 9:28 AM, Martha Ward-Pathology mw...@wakehealth.edu
 wrote:



 We are looking at ways to improve our work processes, save time and labor
 and reduce costs, all while maintaining patient quality...as we all are of
 course.

 During our conversations the subject of cutting unstained slides has come
 up and we are looking for bench marking data to see if we are where we need
 to be.Currently we are cutting unstained slides for various protocols
 (including prostate biopsies), where the specimens are tiny,  but we are
 also cutting a lot of just in case unstained slides.   Our research has
 shown that about 50% of the time the unstained slides requested are never
 used and we are trying to find out if that is high, low or about what other
 institutions are seeing.   If anyone has any data they could share with us
 we would appreciate it.

 Thanks in advance for your help.   I can share what I find out if others
 are interested.


 Martha Ward, MT (ASCP) QIHC
 Manager

 Molecular Diagnostics Lab
 Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f
 336.716.5890 mw...@wakehealth.edu





 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] unstained slides

[Cynthia Pyse]

Hello Histonetters,

 

Recently we have seen an increase number of unstained slides requested from
outside facilities. We do not release our blocks, so unstained slides are
the only option for any facility to obtain our tissue. Currently we do not
charge for these slides, but I am rethinking that policy. What is everyone
doing about unstained slide requests? Are you charging for the slides? How
much per slide?  Any information would be helpful. Thanks in advance.

Cindy

 

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235 Ext. 232

e-mail cp...@x-celllab.com

 

 

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RE: [Histonet] unstained slides

[Lester Raff MD]

Cindy:

If these outside facilities are commercial labs doing proprietary
testing, many of them will pay a set fee to your laboratory for
preparing the slides.  This needs to be negotiated with the commercial
lab rep, but may be in the $40-50 range

Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.492.0203
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Pyse
Sent: Wednesday, August 17, 2011 7:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] unstained slides

Hello Histonetters,

 

Recently we have seen an increase number of unstained slides requested
from
outside facilities. We do not release our blocks, so unstained slides
are
the only option for any facility to obtain our tissue. Currently we do
not
charge for these slides, but I am rethinking that policy. What is
everyone
doing about unstained slide requests? Are you charging for the slides?
How
much per slide?  Any information would be helpful. Thanks in advance.

Cindy

 

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235 Ext. 232

e-mail cp...@x-celllab.com

 

 

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RE: SPAM-LOW: Re: [Histonet] Unstained Slides

[Patsy Ruegg]

I do not bake my unstained slides for IHC controls and store them at 4dc.  I
bake them just before use.  They do last longer this way but Richard is
right, there is no telling because it all depends on the fixation, target
protein stability and storage conditions.  It is always a good idea to cut
fresh sections if the stored unstained sections do not perform as expected.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, June 28, 2010 10:55 AM
To: histonet@lists.utsouthwestern.edu;
rick.garnh...@memorialhealthsystem.com
Subject: SPAM-LOW: Re: [Histonet] Unstained Slides

We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT.
There is no set rule for stability.  It all depends on fixation, the nature
of the protein target, and storage conditions.  I've seen absolutely
spectacular immunoreactivity on unstained slides stored at RT for 20 years
(and longer) and I've seen reduced immunoreactivity in unstained slides
stored for as little as 4 weeks.  We will always attempt to stain unstained
slides when available; however, if the lesion or tumor is negative, and
there is no internal  control, you better cut fresh sections.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 rick.garnh...@memorialhealthsystem.com 6/28/2010 11:54 AM 

Histoland, How  is everyone storing/filing unstained slide. And how long are
they good for to use for immunohistochemistry.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnh...@memorialhealthsystem.com 



Mission: To provide the highest quality health care
Vision: To create an outstanding health system where patients heal and
people thrive
Values: Compassion - Integrity - Quality - Respect - Teamwork

www.memorialhealthsystem.com 

The information contained in or attached to this electronic message is
privileged and confidential, intended only for the use of the individual(s)
named above. If the reader of this message is not the intended recipient, or
the employee or agent responsible to deliver it to the intended
recipient, you are hereby notified that any dissemination, distribution, or
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Re: [Histonet] Unstained Slides

[Rene J Buesa]

Depending on the epitope you are trying to detect, the time could be 
limited from less than one month to 1-2 years.
René J.

--- On Mon, 6/28/10, rick.garnh...@memorialhealthsystem.com 
rick.garnh...@memorialhealthsystem.com wrote:


From: rick.garnh...@memorialhealthsystem.com 
rick.garnh...@memorialhealthsystem.com
Subject: [Histonet] Unstained Slides
To: histonet@lists.utsouthwestern.edu
Date: Monday, June 28, 2010, 11:54 AM



Histoland, How  is everyone storing/filing unstained slide. And how long are 
they good for to use for immunohistochemistry.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnh...@memorialhealthsystem.com



Mission: To provide the highest quality health care
Vision: To create an outstanding health system where patients heal and people 
thrive
Values: Compassion - Integrity - Quality - Respect - Teamwork

www.memorialhealthsystem.com

The information contained in or attached to this electronic message is 
privileged and confidential, intended only for the use of the individual(s)
named above. If the reader of this message is not the intended recipient, or 
the employee or agent responsible to deliver it to the intended
recipient, you are hereby notified that any dissemination, distribution, or 
copying of this communication is strictly prohibited. If you have received
this communication in error, please inform the sender immediately and remove 
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