Re: [Histonet] waterbath/paraffin

[Tony Henwood (SCHN) via Histonet]

Are the tissues taken directly from the molten wax and placed in the 
wax-containing mold or are they allowed to cool before embedding?

One cause of tissue separation is the difference in temperature between tissue 
and embedding wax. 
Try taking directly from molten wax and embedding directly in the wax-filled 
mold.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Betsy Molinari via Histonet 
Sent: Saturday, 26 October 2019 00:49
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] waterbath/paraffin

Hi,
When I place my sections on the waterbath the paraffin pulls away from it. 
Leaving just the edge of the tissue. It is very weird. I have tried at 
different temps . The tissue itself is fine so I do not believe it is the 
processing. I use the same paraffin for processing as well as embedding. I use 
Fisher Histoplast IM.  I was considering melting a block or two down and 
embedding them in another paraffin from another lab.
Suggestions?

Betsy Molinari HT, ASCP
Texas Heart Institute
6770 Bertner Ave.
O-511
Houston, TX 77030
832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
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[Histonet] waterbath/paraffin

[Betsy Molinari via Histonet]

Hi,
When I place my sections on the waterbath the paraffin pulls away from it. 
Leaving just the edge of the tissue. It is very weird. I have tried at 
different temps . The tissue itself is fine so I do not believe it is the 
processing. I use the same paraffin for processing as well as embedding. I use 
Fisher Histoplast IM.  I was considering melting a block or two down and 
embedding them in another paraffin from another lab.
Suggestions?

Betsy Molinari HT, ASCP
Texas Heart Institute
6770 Bertner Ave.
O-511
Houston, TX 77030
832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org | 
facebook | 
twitter

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
authorized, and any such action should not be construed as a waiver of 
privilege or other confidentiality protections.
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