Re: [Histonet] AFB STAIN
I completely agree with Tony. Not likely to see any sloughing off (shedding) of organisms from FFPE tissue blocks. William DeSalvo From: Tony Henwood (SCHN) via Histonet Sent: Thursday, October 4, 2018 1:54 PM To: adesupo2...@hotmail.com; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN Cross-contamination has not been proven in FFPE tissues. I have not seen it in nearly 40 years of practice Do you have any evidence of cross-contamination in FFPE? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Laurie Colbert via Histonet Sent: Thursday, October 4, 2018 10:36 PM To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN You should put the AFB control on a separate slide to prevent cross-contamination. Laurie Redmond -Original Message- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435%7C1%7C0%7C636742832440684556sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435%7C1%7C0%7C636742832440684556sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3Dreserved=0 This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435%7C1%7C0%7C636742832440684556sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB STAIN
Cross-contamination has not been proven in FFPE tissues. I have not seen it in nearly 40 years of practice Do you have any evidence of cross-contamination in FFPE? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Laurie Colbert via Histonet Sent: Thursday, October 4, 2018 10:36 PM To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN You should put the AFB control on a separate slide to prevent cross-contamination. Laurie Redmond -Original Message- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB STAIN
We always try to put them in the same slide, but sometimes it doesn’t work out the way. Either would be acceptable. Sent from my iPhone > On Oct 4, 2018, at 12:35 AM, Tony Henwood (SCHN) via Histonet > wrote: > > We do, > > It works well > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -Original Message- > From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, 4 October 2018 12:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AFB STAIN > > Hello, > I have a question please. For the AFB Stain, do we put both the > control tissue and the patient on the same slide? > > Thanks, > Ade > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB STAIN
We do, It works well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 4 October 2018 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Stain on Cytospin Preps
Greg, I'm not sure if anyone has responded to your question, the waxy coat of acid-fast bacilli is sensitive to xylene and alcohol not to acid. Carbol Fuchsin is soluble in acid alcohol but if the waxy coat of the bacteria is intact, it will hold the stain. What I've suggested before is a cyto-spin preparation of unfixed fluid avoiding any alcohol or xylene even for dehydration and clearing. Hope this helps. J Good day colleagues, We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on a alcohol-fixed cytospin preparation from a cytology fluid. Typically in Cytology, if a specimen is very bloody, we will add acetic acid to lyse the red cells. Does anyone know if acetic acid would have any adverse affect on the stainability of AFB organisms if present? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Control Question
Pam, I worked with our microbiology department and had them to obtain some positive organisms and with our surgery and morgue for fresh tissue. We then inoculate the tissue and process it. Positive blocks are kept on hand and used. We also pass a copy of the slides back to the microbiology department to keep on file as a good batch. It seems to work for us in the educational setting. We have done afb, gms, and gram. Our next batch will include Cryptococcus, and h. pylori. Hope that helps! Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. -- Message: 2 Date: Wed, 15 Feb 2017 15:53:07 + From: "Marcum, Pamela A"To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] AFB Control Question Message-ID: <8c03b2f73b8e4e5593bf93faa4069...@mail13m2n1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Good morning, We are attempting to find AFB controls from human sources. We would trade if we have something you need and we have available. Does anyone have blocks they might share or trade without upsetting our HIPPA people on either side. I am really not interested in buying slides as we cut larger blocks down and have controls on with the patient tissue. Also the control slides are general in the middle of the slide and force to use two slides for every test (too many ordering and all want a control) which makes the cost prohibitive. Our pathologists prefer human tissue and most of the companies I talk to either don't know or won't say it is animal origin or human. -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB contaminant - OR - Monkish Preparation
I slways filtered my air (5um) and my water (0.22um). Only tap water rinses for Hemnatoxylin, Schiff's and other rinses were not rinsed. All of my MolBio and Immuno- stuff was done with filtered (clean?) water at least. The rule is: be Monkish at the bench and as messy as you like in your bedroom (if you are a male and only until you are married), Cheers, Fred Inside your water supply, lurking behind the water fawcet, is a pipe who's surface is lined with a BIO-film. Further horrors await the scientist who fixes and studies the washer in the fawcet whose seal is the better for the 'FILM-m.' Evolution provides variation, and every new flower that grows provides a new isolated environment for speciation. My alimentary biome is unique as we are all unique. Yukky, but true. Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmon...@wcupa.edu From: Abbott, Tanya tanyaabb...@catholichealth.net Sent: Thursday, July 9, 2015 3:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB contaminant
We had some a few years ago and thought it was contaminating bacteria, but turned out to be dirty water from the di tap. We had our plumbers take it apart and clean everything. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Abbott, Tanya [mailto:tanyaabb...@catholichealth.net] Sent: Thursday, July 09, 2015 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB contaminant
Hi, This may be AFB contamination from tap water. The organisms grow in the end of cold water taps. You should take the taps apart and thoroughly descale and scrub out. The organisms may be somewhat longer and thicker than TB, and may be quite distinctly beaded. Bryan Llewellyn Abbott, Tanya wrote: Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB contaminant
most likely a fungus ball, confirm with a PAS Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you From: Abbott, Tanya [tanyaabb...@catholichealth.net] Sent: Thursday, July 09, 2015 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB stained smears
U r right ffpe control would not be ideal but unless u have access to a source + for tb u could make a bunch of smears to keep for controls that may be your only option Sent from my iPhone On Nov 7, 2014, at 10:38 AM, Glenn Hauck glenn.ha...@albertahealthservices.ca wrote: The odd time I receive a request to do a Zeihl Neelsen stain for acid fast bacteria on a smear for Micro. My question is what kind of control should I be using? I have tissue processed with AFB, but I feel this should not be used because the smear is not processed the same way as my AFB control block. Thanks Glenn Hauck Charge Technologist Pathology Queen Elizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.ha...@albertahealthservices.ca This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AFB Controls
Sigma provides AFB control slides if you can't get block. The item number is A2299 Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, October 10, 2013 4:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Controls Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Controls
I know source medical products has them cheaper than newcomer supply. E-mail and ask for Walter. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Thu, Oct 10, 2013 at 5:24 PM, Cheryl tkngfl...@yahoo.com wrote: Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Controls
Make your own. Take some fresh lung (slightly edematous is better, if you can get it). Cut into 2x2 mm cubes (or largest 3x3 mm cubes). Contact Microbiology, and have them make a broth with a non-pathogenic AFB in a large tube (e.g., plastic centrifuge tube). Put lung cubes in broth. Incubate overnight (I found that room temp is usually OK). Next morning, add 10% NBF. Wait about an hour or so, then dispose of NBF, and add fresh 10% NBF. (The first NBF is diluted by the broth, and by allowing the NBF to sit in the broth for a while, it kills the bacteria. Adding the 2nd NBF allows the tissue to be fixed in 10% NBF, rather than diluted NBF.) Allow to fix most of the day, put tissue in cassettes, process as usual, embed, and you have lots of AFB controls for really cheap. Write up a cost containment (how much it cost you to make X number of blocks that you can get Y number of control slides from vs. the cost of buying the same number of Y slides from a vendor.) Management will love you for your cost containment. Works for gram +, gram -, and fungus (get the correct broth). Will work for spirochete too, but our micro lab has only a LARGE non-pathogenic spirochete, which is much larger than syphilis. So doing a Steiner stain would most likely yield a false-negative. (When the large spirochetes control is seen, the little syphilis would not be stained.) Please realize, these controls may not work for IHC. Better check on the genus. They work great for Kinyoun/Ziehl-Neelsen/Fites, Brown and Hopps/Brown and Brenn, GMS/PAS, Steiner/Warthin-Starry. Peggy Wenk, HTL(ASCP)SLS -Original Message- From: Cheryl Sent: Thursday, October 10, 2013 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Controls Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AFB control
If you join the Michigan Society of Histotechnologists for $20/year membership, you will receive not only the award winning MikroGraf newsletter, voted #1 by the NSH, but have access to a supply of control blocks as needed for no extra charge! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cynthia Robinson [robin...@mercyhealth.com] Sent: Wednesday, November 07, 2012 9:20 AM To: histonet Subject: [Histonet] AFB control All, Does anyone have AFB control in human tissue they would be willing to share? I shared fungal controls awhile back with anyone who emailed methat is, until I ran out. I would be willing to share control material if I have something you need. Thanks. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB by IHC
Amy: I think you should clarify with your pathologist this request because AFB, as you perfectly know, is a procedure in itself. Is it that s/he wants to detect acid fast bacteria with an IHC method instead of the Ziehl-Nielsen method? In that case your only option is to look in the IHC providers catalogs if an antibody has been developed for the different types of Mycobacteria and use it. Perhaps your pathologist has found out that H. pyloris are now detected in many labs using IHC and s/he wants to do the same with Mycobacteria. You will need to get a clarification about that request. René J. --- On Tue, 4/3/12, Amy Self as...@georgetownhospitalsystem.org wrote: From: Amy Self as...@georgetownhospitalsystem.org Subject: [Histonet] AFB by IHC To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Tuesday, April 3, 2012, 11:48 AM My pathologist wants an AFB stain done by IHC. Is this stain/procedure even doable and if so where? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB by IHC
Yes, you can do IHC for mycobacteria; however, there are issues with specificity depending on the primary antibody that you use. I have been using a polyclonal antibody from Dako (no longer available) for many years and although it is very sensitive for identifying mycobacteria, it does cross-react with other bacteria. I have not found a monoclonal antibody (yet) that works on formalin-fixed, paraffin-embedded tissue. In addition, your IHC assay has to be exquisitely clean in order to trust the rare immunoreactivity that may be present. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Amy Self as...@georgetownhospitalsystem.org 4/3/2012 11:48 AM My pathologist wants an AFB stain done by IHC. Is this stain/procedure even doable and if so where? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB control
All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS René J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker srhthac...@hotmail.com wrote: From: Stephanie Hoyle-Thacker srhthac...@hotmail.com Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AFB control
It is also a requirement if you are doing work in or for the state of New York. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, February 10, 2012 2:49 PM To: Jennifer MacDonald; Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] AFB control Recommended by who? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, February 10, 2012 2:43 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB control It is recommended to run a negative AFB control to rule out false positives. Some sources of water have acid fast organisms, not TB, and if the water is used in the water baths or for staining you could pick up those organisms. The recommendation is to cut a section of tissue, known NOT to contain AFB organisms, and run it through with the positive control and patient. You would pick it up on the same water bath that you used for the patient. Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 02/10/2012 11:55 AM To histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker srhthac...@hotmail.com cc Subject Re: [Histonet] AFB control All I recall about controls in HC procedures (any of them) is to have a (+) control. The only one that requires both (-) and (+) is PAS René J. --- On Fri, 2/10/12, Stephanie Hoyle-Thacker srhthac...@hotmail.com wrote: From: Stephanie Hoyle-Thacker srhthac...@hotmail.com Subject: [Histonet] AFB control To: histonet@lists.utsouthwestern.edu Date: Friday, February 10, 2012, 1:52 PM Is anyone using a negative control for AFB stains? If so is there a regulation that states negative controls should be run for AFB stains. I seem to recall seeing such a regulation but cannot find it. Thanks for your help! Renee Thacker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AFB
I thought Fite's was more specific for leprosy.. whatever that bug is called now. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Saturday, October 16, 2010 10:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB
Hi - Can't find the original question - must have deleted it, so I'll answer through Cheryl's, if that's OK with her. If I remember, the question was something like - are there other microorganisms besides TB that stain with Kinyoun (or something like that). First, any mycobacterium (acid fast bacteria AFB) will stain with Ziehl-Neelsen, Kinyoun, Auramine-Rhodamine or Fites (hence anything staining with these stains is called AFB positive, meaning staining with these AF stains) . So that means Mycobacterium tuberculosis (TB), Mycobacterium avium intracellular (found in birds and I've seen it in spleen and bone marrow of immune suppressed patients), Mycobacterium paratuberculosis (found in intestine of infected cows and goats, causing something similar to Crone's disease), and a lot of other Mycobacterium are AFB positive staining with these procedures. Mycobacterium leprae (leprosy) is a very thin walled mycobacterium, so needs the Fites stain with the peanut oil (or any other oil, such as mineral oil) to coat the leprosy microorganism, so it can withstand decolorization with aqueous alcohol. But Fites can also be used to demonstrate any of the Mycobacterium. These AFB stains will also demonstrate the sulfur clubs of actinomyces, and the filamentous strands of nocardia are also weakly AFB positive. (Sort of bacteria with some fungus characteristics.) Cryptosporidium found in the intestine (causing severe diarrhea) can also stain AFB positive. And there are bacterium (mycobacterium or others) in tap water that are AFB positive, which can adhere to your slides from the flotation bath water, or from the tap water. Usually non-pathogenic AFB, but I don't know their names. And I'm sure there are other less common bacteria that I've forgotten or never known about that will stain AFB positive, which are not in the Mycobacterium genus. And let's remember that hair follicles, sperm (heads with lipids), lipofucsin (wear and tear pigment of lipids) also stain AFB positive. And also Russell bodies (inclusions of antibodies in plasma cells) will stain AFB positive. And keratin which is dense can simply require longer differentiation, or else it will retain the red color of the dye. So, yes, Amy, there are other things that stain positive with Kinyoun that are not TB/mycobacterium. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Cheryl Crowder ccrow...@vetmed.lsu.edu Sent: Saturday, October 16, 2010 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] afb contamination
You can eliminate the crossover contamination by performing the stain horizontally on a staining rack. A lot of labs re-use the carbol fuchsin, which can have lots of extraneous AFB floating around, waiting patiently to attach itself to an unsuspecting patient slide. Using the staining rack, you don't use a lot of reagent, and you never cross-contaminate. From: Tench, Bill bill.te...@pph.org To: histonet@lists.utsouthwestern.edu Date: 05/21/2010 02:34 PM Subject: [Histonet] afb contamination Sent by: histonet-boun...@lists.utsouthwestern.edu We had a problem with contamination on our AFB stains, and we discovered that it was the control slide flaking off in the copland jar which was being used for staining the control and target slide at the same time (makes sense as a real control'). We identified these contaminants because they were frequently large clusters (by large I would say 4-8 organisms) which we almost never see in real cases, and fortunately, they were also not in the same plane of focus (but that can be subtle). they did create problems. Our solution was to stain the control separately from the case. No more problems. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AFB Control Blocks
Concerning Control Tissue Blocks: The NSH provides and Share and Exchange Service for members and non-members alike. Anytime you have a need for a particular type of control, please contact the NSH office (nsh.org) and we will try and meet your needs. The program is only intended for individual lab use and not commercial use (selling control slides). If you have any questions you can direct them to me and I will assist in any way I can. William DeSalvo, B.S., HTL(ASCP) Chair, NSH Quality Control Committee From: jackd...@msn.com To: histonet@lists.utsouthwestern.edu Date: Thu, 22 Oct 2009 15:31:10 -0400 Subject: [Histonet] AFB Control Blocks Can anyone help me in getting some good AFB control blocks? I have Pneumocystis Controls Blocks for trade. Help! _ Windows 7: It helps you do more. Explore Windows 7. http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen3:102009 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows 7: It works the way you want. Learn more. http://www.microsoft.com/Windows/windows-7/default.aspx?ocid=PID24727::T:WLMTAGL:ON:WL:en-US:WWL_WIN_evergreen2:102009 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet