That decision needs to be made by your Medical Director. In my laboratory we
use polymer detection for almost all of our IHCs and, therefore, I don't
require a Negative Reagent Control for those cases. We have one antibody
that requires avidin-biotin detection and we run a negative reagent control in
parallel whenever we run that antibody. In my experience, if you are using
polymer detection and your antibodies are properly optimized and validated, the
negative reagent control slide serves no useful purpose and, therefore, is not
needed.
Best wishes to everyone on Histonet for the coming New Year!
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-2204 Fax
jsjurc...@comcast.net 12/28/2012 3:18 PM
How is everyone interpreting the CAP rule about using negative controls? Do we
still need a negative with each patient slide?
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