RE: [Histonet] Ebola
http://news.discovery.com/human/health/why-ebola-wont-spread-in-the-united-states-141009.htm http://www.voanews.com/media/video/us-doctors-learn-from-treating-ebola-patients/2477893.html http://www.washingtonpost.com/lifestyle/travel/tips-for-traveling-to-africa-in-the-age-of-ebola/2014/10/09/46af8ae8-4efd-11e4-8c24-487e92bc997b_story.html In our Emory news today... I haven't listened yet, but you all might like to look... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 5:32 PM To: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu); 'Finley, Sue [PH]' Subject: RE: [Histonet] Ebola Correction, due to years of formalin abuse, it was 1 hour in concentrated formic acid. We then processed and cut them with all other cases. A few cases slipped through with just formalin fixation and additional precautions needed to be taken. That said the easiest and primary route of infection for CJD is ingestion. Needless to say we did not eat any of the tissue. A simple and effective method for inactivating virus infectivity in formalin‐fixed tissue samples from patients with Creutzfeldt‐Jakob disease Paul Brown, MD, Axel Wolff, DVM and D. Carleton Gajdusek, MD -Show Affiliations Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. doi: 10.1212/WNL.40.6.887 Neurology June 1990 vol. 40 no. 6 887 Abstract Full Text (PDF) Abstract We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD. © 1990 by the American Academy of Neurology James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 1:57 PM To: 'Emily Brown' Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg prueg...@hotmail.com wrote: Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas
Re: [Histonet] Ebola
If formalin didn't kill CJD, what did you use? Just curious. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg prueg...@hotmail.com wrote: Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Thu, 9 Oct 2014 20:26:16 + From: mucra...@comcast.net To: tanyaabb...@catholichealth.net Subject: Re: [Histonet] Ebola CC: histonet@lists.utsouthwestern.edu Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam - Original Message - From: Tanya Abbott tanyaabb...@catholichealth.net To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, October 9, 2014 2:03:47 PM Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ebola
Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg prueg...@hotmail.com wrote: Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Thu, 9 Oct 2014 20:26:16 + From: mucra...@comcast.net To: tanyaabb...@catholichealth.net Subject: Re: [Histonet] Ebola CC: histonet@lists.utsouthwestern.edu Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam - Original Message - From: Tanya Abbott tanyaabb...@catholichealth.net To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, October 9, 2014 2:03:47 PM Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ebola
Actually it has been proven that formalin will inactivate Ebola, as well as other hemorrhagic fever viruses. Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 09, 2014 4:34 PM To: Pam Marcum; Tanya Abbott Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Thu, 9 Oct 2014 20:26:16 + From: mucra...@comcast.net To: tanyaabb...@catholichealth.net Subject: Re: [Histonet] Ebola CC: histonet@lists.utsouthwestern.edu Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam - Original Message - From: Tanya Abbott tanyaabb...@catholichealth.net To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, October 9, 2014 2:03:47 PM Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ebola
Correction, due to years of formalin abuse, it was 1 hour in concentrated formic acid. We then processed and cut them with all other cases. A few cases slipped through with just formalin fixation and additional precautions needed to be taken. That said the easiest and primary route of infection for CJD is ingestion. Needless to say we did not eat any of the tissue. A simple and effective method for inactivating virus infectivity in formalin‐fixed tissue samples from patients with Creutzfeldt‐Jakob disease Paul Brown, MD, Axel Wolff, DVM and D. Carleton Gajdusek, MD -Show Affiliations Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. doi: 10.1212/WNL.40.6.887 Neurology June 1990 vol. 40 no. 6 887 Abstract Full Text (PDF) Abstract We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD. © 1990 by the American Academy of Neurology James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 1:57 PM To: 'Emily Brown' Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg prueg...@hotmail.com wrote: Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Thu, 9 Oct 2014 20:26:16 + From: mucra...@comcast.net To: tanyaabb...@catholichealth.net Subject: Re: [Histonet] Ebola CC: histonet@lists.utsouthwestern.edu Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam - Original Message - From: Tanya Abbott tanyaabb...@catholichealth.net To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, October 9, 2014 2:03:47 PM Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error
RE: [Histonet] Ebola
We tried very hard not to do CJD cases if we could help it, as I recall any suspect cases were sent to Case Western Reserve, i worked with someone there who developed a protocol using strong formic acid, it was amazing how good the tissue preservation was using formic acid as a fixative. I heard about possible cases in our histo dept at the u where they ended up throwing away microtomes and other instruments who might have come in contact with CJD tissue, crazy, wasn't my department so I was not up on all the details. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: jwat...@gnf.org To: histonet@lists.utsouthwestern.edu; sfin...@providencehealth.bc.ca Date: Thu, 9 Oct 2014 21:31:38 + Subject: RE: [Histonet] Ebola CC: Correction, due to years of formalin abuse, it was 1 hour in concentrated formic acid. We then processed and cut them with all other cases. A few cases slipped through with just formalin fixation and additional precautions needed to be taken. That said the easiest and primary route of infection for CJD is ingestion. Needless to say we did not eat any of the tissue. A simple and effective method for inactivating virus infectivity in formalin‐fixed tissue samples from patients with Creutzfeldt‐Jakob disease Paul Brown, MD, Axel Wolff, DVM and D. Carleton Gajdusek, MD -Show Affiliations Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. doi: 10.1212/WNL.40.6.887 Neurology June 1990 vol. 40 no. 6 887 Abstract Full Text (PDF) Abstract We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD. © 1990 by the American Academy of Neurology James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 1:57 PM To: 'Emily Brown' Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg prueg...@hotmail.com wrote: Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Thu, 9 Oct 2014 20:26:16 + From: mucra...@comcast.net To: tanyaabb...@catholichealth.net Subject: Re: [Histonet] Ebola CC: histonet@lists.utsouthwestern.edu Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam - Original Message - From: Tanya Abbott tanyaabb
