Re: [Histonet] Frozen sections and cold acetone

[Hobbs, Carl via Histonet]

I have always "fixed" in RT acetone for 10 mins
Have compared 0-60 mins/4C to RT acetone
Lower temps just limit the rate of reaction, imho.
I note "nuclear streaming" when I use acetone at any temp/time.

Imho, acetone is not an effective  fixativeit's a delipidiser.
So, give it 10 mins at RT.
If anyone thinks it's effective because it is a dehydrant….well, one re- 
hydrates the section to carry out IHC/ICC/IF.

It works very well for only a few abs.

When testing new abs out on FS/cell monolayers ( unfixed) I always compare 
Formalin, Acetone, Methanol and methanol/acetone 1:1
Imho

Best wishes to a great site/membership.
  
 
Carl 
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Re: [Histonet] Frozen sections and cold acetone...

[Bryan Llewellyn via Histonet]

Cold acetone, and cold ethanol, were used to fix tissues because they 
left enzymes unaffected and still demonstrable. This was in the early 
days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and 
applied,3rd edition, volume 1, page 85 discusses it. I could send a scan 
if you wanted.


Bryan Llewellyn



Morken, Timothy via Histonet wrote:

Can anyone give me a rational for using cold (refrig or freezer-temp) acetone 
to fix frozen sections? Or a rational for using RT acetone.

This is for kidney or muscle bx frozens for immmunofluroescence or 
immunoperoxidase staining.

Normally they air dry for at least 15 minutes (just waiting for frozen 
sectioning to be completed) before going into acetone. Just wondering if we can 
reduce complexity...

I haven't seen anything saying why cold acetone is used, just instructions to 
do so. I always wonder about such things...

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] frozen sections

[Normington Lacy via Histonet]

My institution is an academic medical center; we have PA's, residents, specimen 
receiving technicians and histology technicians performing frozen sections.  We 
perform a yearly competency of frozen sectioning for non MDs.

Lacy Normington

Lacy Normington, HTL(ASCP)CM
Manager, Surgical Pathology Lab Services
UW Health - UW Hospital
600 Highland Avenue
Madison, WI 53792-2472



-Original Message-
From: Eck, Allison via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, May 30, 2018 7:25 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] frozen sections

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




Question for those of you who do not have PA's...Who cuts the frozen sections, 
the techs or the pathologists?

Thanks in advance
Allison

Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1
Lead Tech Histology
Doylestown Hospital
595 W State St
Doylestown, PA 18901
215-345-2264
a...@dh.org


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Re: [Histonet] frozen sections

[Larry Woody via Histonet]

Then you will need AR.
Sent from Yahoo Mail on Android 
 
  On Tue, Jun 20, 2017 at 5:43 PM, Nair, Indu<in...@coh.org> wrote:   

The tissue has been fixed in 4% PFA. 

Thank you!


indu

From: Larry Woody via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 20, 2017 5:30 PM
To: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] frozen sections

Tissue that does not go through formalin processing does not need AR.

Sent from Yahoo Mail on Android

  On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via 
Histonet<histonet@lists.utsouthwestern.edu> wrote:  Yes.
Sent from Yahoo Mail on Android

  On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via 
Histonet<histonet@lists.utsouthwestern.edu> wrote:
Hello Histoneters!

question: Does frozen section need Antigen Retrieval?

thank you, for the anticipated timely help!

indu


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Re: [Histonet] frozen sections

[Nair, Indu via Histonet]

The tissue has been fixed in 4% PFA. 

Thank you!


indu

From: Larry Woody via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 20, 2017 5:30 PM
To: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] frozen sections

Tissue that does not go through formalin processing does not need AR.

Sent from Yahoo Mail on Android

  On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via 
Histonet<histonet@lists.utsouthwestern.edu> wrote:   Yes.
Sent from Yahoo Mail on Android

  On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via 
Histonet<histonet@lists.utsouthwestern.edu> wrote:
Hello Histoneters!

question: Does frozen section need Antigen Retrieval?

thank you, for the anticipated timely help!

indu


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Re: [Histonet] frozen sections

[Larry Woody via Histonet]

Tissue that does not go through formalin processing does not need AR.

Sent from Yahoo Mail on Android 
 
  On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via 
Histonet wrote:   Yes. 
Sent from Yahoo Mail on Android 
 
  On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via 
Histonet wrote:  
Hello Histoneters!

question: Does frozen section need Antigen Retrieval?

thank you, for the anticipated timely help!

indu


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Re: [Histonet] frozen sections

[Isabel Soto via Histonet]

Yes. 
Sent from Yahoo Mail on Android 
 
  On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via 
Histonet wrote:   
Hello Histoneters!

question: Does frozen section need Antigen Retrieval?

thank you, for the anticipated timely help!

indu


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RE: [Histonet] Frozen Sections

[Jeffrey Robinson]

I worked in a hospital Histology Department years ago and the histotechs did 
everything except orient the specimen and read the slide.  The group I work for 
now perform all of their own frozens.  I still cut ORO controls on the cryostat 
but that's about it.  I do have some concerns that the younger histo staff 
members have ZERO experience with frozens.  It's a problem if a pathologist 
does ask for some assistance when there is an issue (especially with 
troubleshooting a cryostat).

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, February 09, 2015 7:03 AM
To: 'wanda.sm...@hcahealthcare.com'; lsmal...@juno.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections


As long as there is a Histotech on duty we cut, stain and coverslip all  the 
frozens, after hours the Pathologists do their own.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
wanda.sm...@hcahealthcare.com
Sent: Monday, February 09, 2015 9:21 AM
To: lsmal...@juno.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

We have 4 Pathologist and all four cut their own FS's.  Two will stain and 
coverslip them themselves and two request assistance with staining and 
coverslipping.  We label and set-up the cassette and slides.
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 10:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2

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RE: [Histonet] Frozen Sections

[Houston, Ronald]

we do, during the day Monday - Friday (8:00-17:30) but not over the weekend

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Monday, February 09, 2015 9:05 AM
To: 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

We provide assistance while the dept. is staff with histology techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 9:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.netzero.net_-3Frefcd-3DNZINTISP0512T4GOUT2d=AwIFAgc=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZm=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4s=yq4UN13f5ayvWibRncCpaEILkARO2iTLjQcW_sJfviYe=
 

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RE: [Histonet] Frozen Sections

[Rathborne, Toni]

We will provide assistance weekdays with staff that accession and stain the 
case.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Monday, February 09, 2015 10:51 AM
To: Mike Pence; 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

we do, during the day Monday - Friday (8:00-17:30) but not over the weekend

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Monday, February 09, 2015 9:05 AM
To: 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

We provide assistance while the dept. is staff with histology techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 9:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.netzero.net_-3Frefcd-3DNZINTISP0512T4GOUT2d=AwIFAgc=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZm=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4s=yq4UN13f5ayvWibRncCpaEILkARO2iTLjQcW_sJfviYe=
 

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RE: [Histonet] Frozen Sections

[Mike Pence]

We provide assistance while the dept. is staff with histology techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 9:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2

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RE: [Histonet] Frozen Sections

[Joelle Weaver]

same


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
 From: mpe...@grhs.net
 To: lsmal...@juno.com; histonet@lists.utsouthwestern.edu
 Date: Mon, 9 Feb 2015 14:04:57 +
 Subject: RE: [Histonet] Frozen Sections
 CC: 
 
 We provide assistance while the dept. is staff with histology techs.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 lsmal...@juno.com
 Sent: Friday, February 06, 2015 9:41 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Frozen Sections
 
 Hi, I need to ask Histoland a questionHow many HT departments provide 
 assistance to the pathologist in the performance of frozen sections (cutting 
 and staining of slides) to be evaluated by the pathologist?   Thank you very 
 much in advance!  
 Lorraine
 
 
 Fast, Secure, NetZero 4G Mobile Broadband. Try it.
 http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2
 
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RE: [Histonet] Frozen Sections

[Wanda.Smith]

We have 4 Pathologist and all four cut their own FS's.  Two will stain and 
coverslip them themselves and two request assistance with staining and 
coverslipping.  We label and set-up the cassette and slides.
Wanda

WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

This email and any files transmitted with it may contain PRIVILEGED or 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 10:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
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Re: [Histonet] Frozen Sections

[histot...@imagesbyhopper.com]

We provide assistance, primarily on the outpatient frozens, but help with 
in-patient ones as well.

What makes you ask?

Michelle

Sent from my iPhone

On Feb 6, 2015, at 10:37 PM, lsmal...@juno.com lsmal...@juno.com wrote:

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2

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Re: [Histonet] Frozen Sections Falling Off Slides

[Badz Yahoo]

Hello netters...have someone tried doin frozen in a tissue mistakenly immersed 
in formalin??not so long but the inside still fresh..m sure the slides will 
wash out..fresh always better..

On Jun 17, 2012, at 10:34 PM, Emily Sours talulahg...@gmail.com wrote:

 Wow you can run frozen sections through those machines? That's awesome.  I
 figured it wouldn't be gentle enough to process them.
 I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
 slides are electrostatically charged to keep the sections from falling
 off.  Whether that's a bunch of hooha or it's actually true, our sections
 don't fall off, even after in situs.  I believe most scientific suppliers
 sell their own brand of superfrost plus slides if you don't want to buy
 from Fisher.
 
 Emily
 
 You see a peanut, day's off to a good start; you witness some soil it's a
 jamboree for Vince Noir.
 --Howard Moon, in Charlie, The Mighty Boosh
 
 
 
 On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio 
 one_angel_sec...@yahoo.comwrote:
 
 Was your leica set that cold? Maybe you could try warming the slide for a
 couple seconds before putting in alcohol. As far a charged slides I am
 amazed with the slides Dako sells for their IHC.
 
 Sent from my iPhone
 
 On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com
 wrote:
 
 Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
 section Machine...But i dont understand why i got problems sections falling
 off slides.I used different types of slides non adhesive,adhesive(poly l
 lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
 from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
 etc..then after distilled water wash out...sections started to peel
 off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
 tissue- fibroma and salivary gland...
 I used Leica cryostat previously but i never got these problems...Even i
 used non adhesive but sections still on the slides..Can someone advised
 please.Maybe youre using the same tissue tek cryo 3...
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RE: [Histonet] Frozen Sections Falling Off Slides

[Ingles Claire]

Just remember - No formalin, no need for retrieval. In my experience that is 
the hardest step on tissue sections. It also shortens the procedure a bunch 
too. 
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Emily Sours
Sent: Sun 6/17/2012 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen Sections Falling Off Slides



Wow you can run frozen sections through those machines? That's awesome.  I
figured it wouldn't be gentle enough to process them.
I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
slides are electrostatically charged to keep the sections from falling
off.  Whether that's a bunch of hooha or it's actually true, our sections
don't fall off, even after in situs.  I believe most scientific suppliers
sell their own brand of superfrost plus slides if you don't want to buy
from Fisher.

Emily

You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh



On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio one_angel_sec...@yahoo.comwrote:

 Was your leica set that cold? Maybe you could try warming the slide for a
 couple seconds before putting in alcohol. As far a charged slides I am
 amazed with the slides Dako sells for their IHC.

 Sent from my iPhone

 On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com
 wrote:

  Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
 section Machine...But i dont understand why i got problems sections falling
 off slides.I used different types of slides non adhesive,adhesive(poly l
 lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
 from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
 etc..then after distilled water wash out...sections started to peel
 off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
 tissue- fibroma and salivary gland...
  I used Leica cryostat previously but i never got these problems...Even i
 used non adhesive but sections still on the slides..Can someone advised
  please.Maybe youre using the same tissue tek cryo 3...
  ___
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Re: [Histonet] Frozen Sections Falling Off Slides

[Emily Sours]

Wow you can run frozen sections through those machines? That's awesome.  I
figured it wouldn't be gentle enough to process them.
I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
slides are electrostatically charged to keep the sections from falling
off.  Whether that's a bunch of hooha or it's actually true, our sections
don't fall off, even after in situs.  I believe most scientific suppliers
sell their own brand of superfrost plus slides if you don't want to buy
from Fisher.

Emily

You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh



On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio one_angel_sec...@yahoo.comwrote:

 Was your leica set that cold? Maybe you could try warming the slide for a
 couple seconds before putting in alcohol. As far a charged slides I am
 amazed with the slides Dako sells for their IHC.

 Sent from my iPhone

 On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com
 wrote:

  Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
 section Machine...But i dont understand why i got problems sections falling
 off slides.I used different types of slides non adhesive,adhesive(poly l
 lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
 from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
 etc..then after distilled water wash out...sections started to peel
 off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
 tissue- fibroma and salivary gland...
  I used Leica cryostat previously but i never got these problems...Even i
 used non adhesive but sections still on the slides..Can someone advised
  please.Maybe youre using the same tissue tek cryo 3...
  ___
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  Histonet@lists.utsouthwestern.edu
  http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] Frozen Sections Falling Off Slides

[Rene J Buesa]

Although it should NOT be the cause, I always used my cryostat at -20 to -25ºC 
Second I always warmed the slides (at least 30ºC) before picking the sections. 
This requires having a well extended cryosection and to pick them up with a 
smooth movement. You will not have any chance of rearranging-extending the 
section.
As a fixative I used NBF for 30 seconds → to regular or special staining.
René J.



From: Bernadette del Rosario badzros...@yahoo.com
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu 
Sent: Saturday, June 16, 2012 12:01 PM
Subject: [Histonet] Frozen Sections Falling Off Slides

Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section 
Machine...But i dont understand why i got problems sections falling off 
slides.I used different types of slides non adhesive,adhesive(poly l 
lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 
2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then 
after distilled water wash out...sections started to peel off...My machine 
setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and 
salivary gland...
I used Leica cryostat previously but i never got these problems...Even i used 
non adhesive but sections still on the slides..Can someone advised  
please.Maybe youre using the same tissue tek cryo 3...
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Re: [Histonet] Frozen Sections Falling Off Slides

[Kim Donadio]

Was your leica set that cold? Maybe you could try warming the slide for a 
couple seconds before putting in alcohol. As far a charged slides I am amazed 
with the slides Dako sells for their IHC. 

Sent from my iPhone

On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com 
wrote:

 Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section 
 Machine...But i dont understand why i got problems sections falling off 
 slides.I used different types of slides non adhesive,adhesive(poly l 
 lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken 
 from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative 
 etc..then after distilled water wash out...sections started to peel off...My 
 machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- 
 fibroma and salivary gland...
 I used Leica cryostat previously but i never got these problems...Even i used 
 non adhesive but sections still on the slides..Can someone advised  
 please.Maybe youre using the same tissue tek cryo 3...
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Frozen sections

[sgoebel]

Chatter can also be caused by your blade angle.  Try making the angle
more obtuse, just don't over do it or you will get venitian (sp?) blind.
 The temperature is also a little cold.  Try putting your thumb on the
chuck, on top of the tissue, for a few sections before cutting and see
if that helps any.  If the tissue is necrotic, it's kind of like
fat...not much you can do?
Good Luck!!

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: [Histonet] Frozen sections
From: Maria Katleba maria.katl...@stjoe.org
Date: Tue, October 19, 2010 10:27 am
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu

Question: When doing frozen section on a lung mass (not fatty- just
regular), and it chatters, what is the cause?

The cryostat was set at -26, we used the normal OCT media, and cut at
5microns.

I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and
even 6 to see if it would help... still chatter! The pathologist said it
looked all necrotic-like!!

Without knowing for sure if the tissue's morphology is due to its real
state (ie- full of cancer) or a cause of freezing artifact, I need some
help from any histotech that is a Frozen Section Guru :)

Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime


Notice from St. Joseph Health System:
Please note that the information contained in this message may be
privileged and confidential and protected from disclosure.



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Re: [Histonet] frozen sections of cartilage

[Rene J Buesa]

You should aim at obtaining 4 objectives:
1- extremely thin sections (as thin as you are able to produce);
2- absolutely wrinkle free;
3- using positivey charged slides, and
4- let the sections air dry completely before starting the IHC
René J.

--- On Mon, 2/22/10, Kim Merriam kmerriam2...@yahoo.com wrote:


From: Kim Merriam kmerriam2...@yahoo.com
Subject: [Histonet] frozen sections of cartilage
To: Histonet histonet@lists.utsouthwestern.edu
Date: Monday, February 22, 2010, 2:37 PM


Hi Everyone,

Any tips for keeping frozen sections of cartilage from falling off the 
slides during IHC staining?  We should not have to do HIER, but they still fall 
of the slides quite easily.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



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RE: [Histonet] Frozen Sections Slides per Day Question

[soofia siddiqui]

Hi all,
Thank you so much! for all of your suggestions. 
 
 Rene,
How many slides per case, if FS standard is 15 min/case?
 
Thanks again. 
 
Soofia
--- On Sat, 2/6/10, Rene J Buesa rjbu...@yahoo.com wrote:


From: Rene J Buesa rjbu...@yahoo.com
Subject: RE: [Histonet] Frozen Sections Slides per Day Question
To: tahs...@brain.net.pk tahs...@brain.net.pk, soofia siddiqui 
soofi...@yahoo.com, Barry RRittman barry.r.ritt...@uth.tmc.edu
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Saturday, February 6, 2010, 9:19 AM







FS standard is 15 minutes/case.
René J.

--- On Sat, 2/6/10, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote:


From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu
Subject: RE: [Histonet] Frozen Sections Slides per Day Question
To: tahs...@brain.net.pk tahs...@brain.net.pk, soofia siddiqui 
soofi...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Saturday, February 6, 2010, 8:03 AM


While I no longer cut frozen sections, I did so for several years.
The number of blocks and sections you cut depends on so many factors including 
the type of tissue sectioned,  changing knife and roll plate positions, time to 
seal and store blocks after cutting, your expertise  etc.
So that, depending on circumstances, the number can vary considerably from lab 
to lab.
What mostly concerned me about your email was that you would be doing this all 
day.
There are so many injuries that can be caused by repetitive tasks in histology, 
most commonly cutting of frozen and paraffin wax sections.
Apart from this is the potential for errors that occur with continuous 
repetitive tasks and increases with the length of time period in which these 
are done..
I would recommend that multitasking with some other tasks alternating with the 
frozen sections
Just my opinion.
Barry


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk 
[tahs...@brain.net.pk]
Sent: Saturday, February 06, 2010 3:38 AM
To: soofia siddiqui
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen Sections Slides per Day Question

Dear Soofia Sissiqui,
25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units
code # 5019 entire day ( 8 hours 480 minte)
Muhammad Tahseen
Histology Supervisor
SKMTHRC Lahore Pakistan


 Hi dear histology experts,
 I will greatly appreciate if somebody can let me know the estimated number
 of slides (with 3 sections per slide) per day on average a lab technician
 can cut (of frozen tissue).

 I am a lab technician ( not a histotech) and work alone in a highly
 complex testing specialzed low volume dermatology lab.My job
 description includes 30 % of immunohistochemistry related dutites.  I cut
 skin frozen sections and do immunohistochemistry  manually for several
 years . My routiene panel consists of 12 antibodies for  T-cells  surface
 markers. Ocassionally  I add another panel of 8 antibodies for B-Cells.
 I am very slow in cutting sections and strugle a lot to get good sections.
 If I spend entire day ( 8 hours) just  cutting  3-4 section on each slide
 with slow speed. What  number of slides should be considered as efficent
 cutting?
 What number of blocks (12 slides for each block with 3-4 sections) should
 I finish in one day?

 Help me please if you can. Thanks. Soofia




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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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RE: [Histonet] Frozen Sections Slides per Day Question

[Rene J Buesa]

The norm is 2 (max. 3) slides per case.
Your figure of 25 FS in 8 hours = 19.2 min/FS but you do a lot more that just 
2-3 slides/case.
René J

--- On Mon, 2/8/10, soofia siddiqui soofi...@yahoo.com wrote:


From: soofia siddiqui soofi...@yahoo.com
Subject: RE: [Histonet] Frozen Sections Slides per Day Question
To: tahs...@brain.net.pk tahs...@brain.net.pk, Barry RRittman 
barry.r.ritt...@uth.tmc.edu, Rene J Buesa rjbu...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Monday, February 8, 2010, 11:31 AM







Hi all,
Thank you so much! for all of your suggestions. 
 
 Rene,
How many slides per case, if FS standard is 15 min/case?
 
Thanks again. 
 
Soofia
--- On Sat, 2/6/10, Rene J Buesa rjbu...@yahoo.com wrote:


From: Rene J Buesa rjbu...@yahoo.com
Subject: RE: [Histonet] Frozen Sections Slides per Day Question
To: tahs...@brain.net.pk tahs...@brain.net.pk, soofia siddiqui 
soofi...@yahoo.com, Barry RRittman barry.r.ritt...@uth.tmc.edu
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Saturday, February 6, 2010, 9:19 AM







FS standard is 15 minutes/case.
René J.

--- On Sat, 2/6/10, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote:


From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu
Subject: RE: [Histonet] Frozen Sections Slides per Day Question
To: tahs...@brain.net.pk tahs...@brain.net.pk, soofia siddiqui 
soofi...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Saturday, February 6, 2010, 8:03 AM


While I no longer cut frozen sections, I did so for several years.
The number of blocks and sections you cut depends on so many factors including 
the type of tissue sectioned,  changing knife and roll plate positions, time to 
seal and store blocks after cutting, your expertise  etc.
So that, depending on circumstances, the number can vary considerably from lab 
to lab.
What mostly concerned me about your email was that you would be doing this all 
day.
There are so many injuries that can be caused by repetitive tasks in histology, 
most commonly cutting of frozen and paraffin wax sections.
Apart from this is the potential for errors that occur with continuous 
repetitive tasks and increases with the length of time period in which these 
are done..
I would recommend that multitasking with some other tasks alternating with the 
frozen sections
Just my opinion.
Barry


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk 
[tahs...@brain.net.pk]
Sent: Saturday, February 06, 2010 3:38 AM
To: soofia siddiqui
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen Sections Slides per Day Question

Dear Soofia Sissiqui,
25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units
code # 5019 entire day ( 8 hours 480 minte)
Muhammad Tahseen
Histology Supervisor
SKMTHRC Lahore Pakistan


 Hi dear histology experts,
 I will greatly appreciate if somebody can let me know the estimated number
 of slides (with 3 sections per slide) per day on average a lab technician
 can cut (of frozen tissue).

 I am a lab technician ( not a histotech) and work alone in a highly
 complex testing specialzed low volume dermatology lab.My job
 description includes 30 % of immunohistochemistry related dutites.  I cut
 skin frozen sections and do immunohistochemistry  manually for several
 years . My routiene panel consists of 12 antibodies for  T-cells  surface
 markers. Ocassionally  I add another panel of 8 antibodies for B-Cells.
 I am very slow in cutting sections and strugle a lot to get good sections.
 If I spend entire day ( 8 hours) just  cutting  3-4 section on each slide
 with slow speed. What  number of slides should be considered as efficent
 cutting?
 What number of blocks (12 slides for each block with 3-4 sections) should
 I finish in one day?

 Help me please if you can. Thanks. Soofia




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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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Re: [Histonet] Frozen Sections Slides per Day Question

[tahseen]

Dear Soofia Sissiqui,
25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units
code # 5019 entire day ( 8 hours 480 minte)
Muhammad Tahseen
Histology Supervisor
SKMTHRC Lahore Pakistan


 Hi dear histology experts,
 I will greatly appreciate if somebody can let me know the estimated number
 of slides (with 3 sections per slide) per day on average a lab technician
 can cut (of frozen tissue).
  
 I am a lab technician ( not a histotech) and work alone in a highly
 complex testing specialzed low volume dermatology lab.My job
 description includes 30 % of immunohistochemistry related dutites.  I cut
 skin frozen sections and do immunohistochemistry  manually for several
 years . My routiene panel consists of 12 antibodies for  T-cells  surface
 markers. Ocassionally  I add another panel of 8 antibodies for B-Cells.
 I am very slow in cutting sections and strugle a lot to get good sections.
 If I spend entire day ( 8 hours) just  cutting  3-4 section on each slide
 with slow speed. What  number of slides should be considered as efficent
 cutting?
 What number of blocks (12 slides for each block with 3-4 sections) should
 I finish in one day?
  
 Help me please if you can. Thanks. Soofia
  



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RE: [Histonet] Frozen Sections Slides per Day Question

[Rittman, Barry R]

While I no longer cut frozen sections, I did so for several years.
The number of blocks and sections you cut depends on so many factors including 
the type of tissue sectioned,  changing knife and roll plate positions, time to 
seal and store blocks after cutting, your expertise  etc.
So that, depending on circumstances, the number can vary considerably from lab 
to lab.
What mostly concerned me about your email was that you would be doing this all 
day.
There are so many injuries that can be caused by repetitive tasks in histology, 
most commonly cutting of frozen and paraffin wax sections.
Apart from this is the potential for errors that occur with continuous 
repetitive tasks and increases with the length of time period in which these 
are done..
I would recommend that multitasking with some other tasks alternating with the 
frozen sections
Just my opinion.
Barry


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk 
[tahs...@brain.net.pk]
Sent: Saturday, February 06, 2010 3:38 AM
To: soofia siddiqui
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen Sections Slides per Day Question

Dear Soofia Sissiqui,
25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units
code # 5019 entire day ( 8 hours 480 minte)
Muhammad Tahseen
Histology Supervisor
SKMTHRC Lahore Pakistan


 Hi dear histology experts,
 I will greatly appreciate if somebody can let me know the estimated number
 of slides (with 3 sections per slide) per day on average a lab technician
 can cut (of frozen tissue).

 I am a lab technician ( not a histotech) and work alone in a highly
 complex testing specialzed low volume dermatology lab.My job
 description includes 30 % of immunohistochemistry related dutites.  I cut
 skin frozen sections and do immunohistochemistry  manually for several
 years . My routiene panel consists of 12 antibodies for  T-cells  surface
 markers. Ocassionally  I add another panel of 8 antibodies for B-Cells.
 I am very slow in cutting sections and strugle a lot to get good sections.
 If I spend entire day ( 8 hours) just  cutting  3-4 section on each slide
 with slow speed. What  number of slides should be considered as efficent
 cutting?
 What number of blocks (12 slides for each block with 3-4 sections) should
 I finish in one day?

 Help me please if you can. Thanks. Soofia




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