RE: [Histonet] HE stain uneven
I had this problem awhile back. Did you change your solvent for deparaffinization? I changed from xylene to another solvent and realized I had to extend the time my slides were in it. Betsy Molinari HT(ASCP) Texas Heart Institue Cardiovascular Pathology 1101 Bates Street Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stain uneven Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stain uneven
Have you checked the temperature of the heater used to dry you slides? We ran into this problem a couple of years ago and the temp of the oven was too high. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 1:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stain uneven Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stain uneven
Check your dewaxing → hydrating protocol and change reagents frequently. René J. --- On Fri, 2/11/11, Madeline Gi madelin...@yahoo.com wrote: From: Madeline Gi madelin...@yahoo.com Subject: [Histonet] HE stain uneven To: Histonet@lists.utsouthwestern.edu Date: Friday, February 11, 2011, 1:44 PM Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
Our tap water consistently reads 6.0, and has for years. We did try turning off the tap when this first began, and manually rinsing with distilled water, but saw no difference. I will try adding the HCl today with a few test slides. Will let you know how this works out. Thanks for the suggestions. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Thursday, January 20, 2011 12:26 AM To: jkier...@uwo.ca; allison_sc...@hchd.tmc.edu Cc: histonet Subject: RE: [Histonet] HE Stain I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using tap water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) From: jkier...@uwo.ca To: allison_sc...@hchd.tmc.edu Date: Wed, 19 Jan 2011 16:58:57 -0500 Subject: Re: [Histonet] HE Stain CC: histonet@lists.utsouthwestern.edu Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050
Re: [Histonet] HE Stain
Allison/Toni, Thought I'd throw this out. Maybe nonsense. If you have such acidic tap water, could there be heavy metals lead, magnesium and others (acid tap water does that) in your tap water rinse from being leached out upstream. William DeSalvo talked about the quality of tap water fluctuating. Very true. And the metals from pipes or solder , leached into water by pH6.0, turning a normal hematoxylin into something like a Weigerts hematoxylin. A kind of post-mordanting that I think some call afterchroming. Although if you tried distilled or deionized water with same results, that data wouldn't fit with this problem. And even if it just started happening, has someone recently worked on pipes upsteam of where you are and there is (are) new metals being leached into your hematoxylin rinse? pH 6 is pretty acidic water. RayKoelling PhenoPath Labs Seattle, WA - Original Message - From: Toni Rathborne trathbo...@somerset-healthcare.com To: WILLIAM DESALVO wdesalvo@hotmail.com, jkier...@uwo.ca, allison scott allison_sc...@hchd.tmc.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 6:17:46 AM Subject: RE: [Histonet] HE Stain Our tap water consistently reads 6.0, and has for years. We did try turning off the tap when this first began, and manually rinsing with distilled water, but saw no difference. I will try adding the HCl today with a few test slides. Will let you know how this works out. Thanks for the suggestions. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Thursday, January 20, 2011 12:26 AM To: jkier...@uwo.ca; allison_sc...@hchd.tmc.edu Cc: histonet Subject: RE: [Histonet] HE Stain I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using tap water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) From: jkier...@uwo.ca To: allison_sc...@hchd.tmc.edu Date: Wed, 19 Jan 2011 16:58:57 -0500 Subject: Re: [Histonet] HE Stain CC: histonet@lists.utsouthwestern.edu Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited
RE: [Histonet] HE Stain
More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stain Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stain Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stain
Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
Hi Toni, I would love to speak with you about the issues that you are having, could you give me a call? I am traveling tomorrow but will be back in the office on Friday. thanks Debbie Siena Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 800-442-3573 ext 229 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, January 19, 2011 12:35 PM To: Mike Pence; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stain We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stain Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using tap water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) From: jkier...@uwo.ca To: allison_sc...@hchd.tmc.edu Date: Wed, 19 Jan 2011 16:58:57 -0500 Subject: Re: [Histonet] HE Stain CC: histonet@lists.utsouthwestern.edu Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
