Re: [Histonet] HT HistoDeck question...
I think the problem with a is that 37% formaldehyde is what is considered 100% formalin not 10% nbf. So for all technical purposes I've never known of anyone fixing samples in 100% formalin either But I would agree it depends on what your doing with the sample if it got acetone or not. So i agree with you if they are asking what the fixative is for the fs to go for perm then they need to clarify and fix what they are saying for option a. Im not sure but it seems like they are trying to pass 37% formaldehyde off as 10% formalin. Anyone else get that feeling? Sent from my iPhone On Oct 3, 2013, at 10:39 AM, McKenzie, Emily mckenzie.em...@mhsil.com wrote: Honestly I think this all depends on what they are actually asking. Are they asking what you fix the tissue in after the frozen is complete and you need to submit the remaining tissue for routine processing? If so, then A is the correct answer. Are they asking what you fix the tissue to the slide with to stain the frozen section for immediate diagnosis? If so, then the answer can be several possibilities depending on what you are going to be staining/ the process you will be staining with. I have worked in several different hospitals and each have a different protocol for there frozen section staining. In order to fix the tissue to the slide I have used 95% alcohol and acidic acid alcohol. The only time I have used formaldehyde is when I use the vapors for fat staining. I think this might be a question to take up with ASCP. They are the ones administering the test. Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center│701 North First Street│Springfield, IL 62781 Ph: 217-788-3991│email: mckenzie.em...@mhsil.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 9:22 AM To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing
Re: [Histonet] HT HistoDeck question...
Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
Thank you All! Sheryl Stephenson | Histology Technician -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, October 03, 2013 9:43 AM To: Watson, Linda; Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution
RE: [Histonet] HT HistoDeck question...
Honestly I think this all depends on what they are actually asking. Are they asking what you fix the tissue in after the frozen is complete and you need to submit the remaining tissue for routine processing? If so, then A is the correct answer. Are they asking what you fix the tissue to the slide with to stain the frozen section for immediate diagnosis? If so, then the answer can be several possibilities depending on what you are going to be staining/ the process you will be staining with. I have worked in several different hospitals and each have a different protocol for there frozen section staining. In order to fix the tissue to the slide I have used 95% alcohol and acidic acid alcohol. The only time I have used formaldehyde is when I use the vapors for fat staining. I think this might be a question to take up with ASCP. They are the ones administering the test. Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center│701 North First Street│Springfield, IL 62781 Ph: 217-788-3991│email: mckenzie.em...@mhsil.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 9:22 AM To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM
Re: [Histonet] HT HistoDeck question...
I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
It would dissolve the fat if you used acetone wouldnt it? Without knowing the tissue type or stain, the answer is A. All other choices dissolve fat I think. Good luck on your exam! Sent from my iPhone On Oct 3, 2013, at 8:15 AM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
the protocol calls for 1 minute in the 40% formaldehyde and then rinse the sections well. From: Rathborne, Toni trathbo...@somerset-healthcare.com To: 'Jennifer MacDonald' jmacdon...@mtsac.edu, Grantham, Andrea L - (algranth) algra...@email.arizona.edu Cc: HISTONET histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date: 10/03/2013 11:42 AM Subject:RE: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
