RE: [Histonet] RE: AFB and negative control
Hi All, According to CLIA Interpretative guidelines, a negative AFB control tissue is to be run each day of testing. §493.1256 Standard: Control procedures. (e)(2) Each day of use (unless otherwise specified in this subpart), test staining materials for intended reactivity to ensure predictable staining characteristics. Control materials for both positive and negative reactivity must be included, as appropriate. Interpretive Guidelines §493.1256(e)(2)-(e)(3) Acid-fast stains must be checked each day of use for positive and negative reactivity. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, March 14, 2013 3:51 PM To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] RE: AFB and negative control This practice is listed as a QC measure for issues of cross contamination in the ASCP publication Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tnma...@mdanderson.org To: histonet@lists.utsouthwestern.edu Date: Thu, 14 Mar 2013 17:52:39 + Subject: [Histonet] RE: AFB and negative control While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Date: Thu, 14 Mar 2013 12:42:30 + From: Ian R Bernard ibern...@uab.edu Subject: [Histonet] AFB and Negative Control To: Lee Peggy Wenk lpw...@sbcglobal.net, Tighe, Sean T sti...@ufl.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=utf-8 The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: AFB and negative control
This practice is listed as a QC measure for issues of cross contamination in the ASCP publication Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tnma...@mdanderson.org To: histonet@lists.utsouthwestern.edu Date: Thu, 14 Mar 2013 17:52:39 + Subject: [Histonet] RE: AFB and negative control While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Date: Thu, 14 Mar 2013 12:42:30 + From: Ian R Bernard ibern...@uab.edu Subject: [Histonet] AFB and Negative Control To: Lee Peggy Wenk lpw...@sbcglobal.net, Tighe, Sean T sti...@ufl.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=utf-8 The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: AFB and negative control
Isn't a gram stain the only special that requires a positive and negative control? Sent from my iPhone On Mar 14, 2013, at 1:51 PM, joelle weaver joellewea...@hotmail.com wrote: This practice is listed as a QC measure for issues of cross contamination in the ASCP publication Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tnma...@mdanderson.org To: histonet@lists.utsouthwestern.edu Date: Thu, 14 Mar 2013 17:52:39 + Subject: [Histonet] RE: AFB and negative control While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Date: Thu, 14 Mar 2013 12:42:30 + From: Ian R Bernard ibern...@uab.edu Subject: [Histonet] AFB and Negative Control To: Lee Peggy Wenk lpw...@sbcglobal.net, Tighe, Sean T sti...@ufl.edu,histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=utf-8 The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
