RE: And other crazy stuff. RE: [Histonet] cutting honey bees
Shrew mandible, spittle bugs, white fly salivary glands, mosquito ovaries. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Paula Sicurello [pat...@gmail.com] Sent: Thursday, January 08, 2015 1:43 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Roberta Horner; Douglas Gregg Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones mjo...@metropath.com wrote: We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
And other crazy stuff. RE: [Histonet] cutting honey bees
You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
we once played an april's fool joke on our Heme Pathologist. We took the wings off a fly and embedded it in plastic (GMA) sectioned and stained it. Grossly It looked similar to the bone marrow core biopsies we did. Heme pathologist are notorious for sticking slides under the microscope on high power without looking at them grossly. We said we needed him to consult on this really strange bone marrow core because we couldn't figure out what the patient had. Most of our patients at the time were patients with leukemia or lymphoma. Sure enough he stuck the slide under high power and probably even under oil immersion. Trying to look at the morphology of individual cells. He was stumped and was going to show it to another colleague, so we had to confess that it was a fly and this was a AF joke. We thought he would have thought we were clever and laugh about it but he did not think it was funny. We never did anything like that again. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com Subject: RE: And other crazy stuff. RE: [Histonet] cutting honey bees Date: Thu, 8 Jan 2015 15:27:58 -0500 From: dhew...@hvhs.org To: mjo...@metropath.com; timothy.mor...@ucsf.edu; prueg...@hotmail.com; r...@psu.edu; classic...@gmail.com; histonet@lists.utsouthwestern.edu I have done a stink bug, spider and a few other creepy crawlers for my kids to look at under the scope, they have no idea what they are looking at but still love it. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Thursday, January 08, 2015 3:20 PM To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg; 'histonet@lists.utsouthwestern.edu' Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
Termites for a science fair project. anchovy from a pizza to pull a joke on a pathologist chicken bone from dim sum chicken feet, again to pull one over on a pathologist. Not decalcified, embedded in resin. From: Morken, Timothy timothy.mor...@ucsf.edu To: Patsy Ruegg prueg...@hotmail.com, Roberta Horner r...@psu.edu, Douglas Gregg classic...@gmail.com, Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Date: 01/08/2015 12:00 PM Subject:And other crazy stuff. RE: [Histonet] cutting honey bees Sent by:histonet-boun...@lists.utsouthwestern.edu You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones mjo...@metropath.com wrote: We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
I have done a stink bug, spider and a few other creepy crawlers for my kids to look at under the scope, they have no idea what they are looking at but still love it. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Thursday, January 08, 2015 3:20 PM To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg; 'histonet@lists.utsouthwestern.edu' Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
You guys are so cool!! Thanks for sharing your stories - I love reading all of the cool stuff histotechs are doing out in the world! Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 5:38 PM, Caroline Miller mi...@3scan.com wrote: When I worked in a research core (which was only last week, but I just changed jobs). I have processed and cut (with varying degrees of success) fake meat samples for a company. They were mainly made of grains and mushed veggies. The problem was the samples were not consistent and there was no room (time or money) for honing the protocol for each of the many samples he sent me, so he got what I could cut. He always seemed pleased, but I couldn't see much in the samples. He was very secretive about it all, so I could never quite understand what they were doing t all for!!! C Sent from my iPhone On Jan 6, 2015, at 12:54 PM, Roberta Horner r...@psu.edu wrote: The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] RE: [Histonet] cutting honey bees
I agree with Patsy. If you have a access to a plastic histo lab, that would be the best, as you could easily section any part of the Bee's anatomy. Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 2:13 PM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: [EXTERNAL] RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting honey bees
for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
When I worked in a research core (which was only last week, but I just changed jobs). I have processed and cut (with varying degrees of success) fake meat samples for a company. They were mainly made of grains and mushed veggies. The problem was the samples were not consistent and there was no room (time or money) for honing the protocol for each of the many samples he sent me, so he got what I could cut. He always seemed pleased, but I couldn't see much in the samples. He was very secretive about it all, so I could never quite understand what they were doing t all for!!! C Sent from my iPhone On Jan 6, 2015, at 12:54 PM, Roberta Horner r...@psu.edu wrote: The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
The coolest thing I cut was 600 yo deer bone. One of our pathologist did archeology as a hobby and wanted me to section it, it was petrified as hard as a rock. We tried everything to soften it to no avail. We ended up cutting with a diamond wire lapidary saw without embedding it in anything. Could make sections as thin as 30 microns. Had a heck of a time trying to get sections to adhere to a glass slide and the sections would not take any stain. We did end up looking at it with a fluorescent scope. We could see rings like a tree of bone turn over. This is how tetracycline labeling of bone first came about. Someone way back stuck a piece of dinosaur bone under UV light and saw rings, apparently the animals eat moldy grain ( tet is made from mold) and it deposits where ever new bone is being laid down, it also happens to fluoresce. I spent 25 years in a metabolic bone disease lab, we treated the patient with a course of tetracycline then waited for a period of time, I think it was 10-14 days, then the patient took another dose of tet and then within a day or 3 we biopsied their bone usually from the illiac crest fixed it in methanol because the tetracycline was water soluble then processed it into GMA plastic without decal. Unstained 5 micron sections cut with a tungsten carbide blade were reviewed with a fluorescent scope revealing the two labels of tet, since we knew the time between doses we could measure the area between the two labels and report them out as bone growth in mm per day. People with severe lack of bone turn over would just have one single label meaning they were not making much bone. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: timothy.mor...@ucsf.edu; prueg...@hotmail.com; classic...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: And other crazy stuff. RE: [Histonet] cutting honey bees Date: Tue, 6 Jan 2015 20:54:10 + The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting honey bees
I processed honeybees and was successful sectioning them. It takes a bit of patience and time. I soaked the bees to soften the outer parts in glycerin water and or mollifex. As for processing, I had to make up a schedule and infiltration is the key. If it isn't important to see the whole bee you can section out the parts you need to see most and then it becomes easy. I'm slowly working on my notes and someday I'll get them all sorted out. Andi G From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Patsy Ruegg [prueg...@hotmail.com] Sent: Tuesday, January 06, 2015 12:13 PM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting honey bees
I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
