You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head.
Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones <mjo...@metropath.com> wrote: > We did a goldfish once, interesting microscopically and difficult for > peeling (lots of keratin?) > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > mjo...@metropath.com > > > > > On 1/6/15, 12:23 PM, "Morken, Timothy" <timothy.mor...@ucsf.edu> wrote: > > >You crazy research people...OK, so what is the craziest thing you ever > >had to cut, or were asked to cut? > > > >For me, not too bad, but embedding for EM and sectioning a single oocyte > >that was nearly microscopic. I'll just say it took a LOT of thick > >sections too face down to it without actually cutting through it. > > > > > >Open the floodgates.... > > > >Tim Morken > > > >-----Original Message----- > >From: histonet-boun...@lists.utsouthwestern.edu > >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy > >Ruegg > >Sent: Tuesday, January 06, 2015 11:13 AM > >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > >Subject: RE: [Histonet] cutting honey bees > > > >for the whole bee I probably would process and embed it in glycol > >methacrylate (gma) it is much harder and would give better sections, we > >have done zebra fish and several other harder tissues including calcified > >bone in GMA. > > > >Cheers, > >Patsy > > > >Patsy Ruegg, HT(ASCP)QIHC > >Ruegg IHC Consulting > >40864 E Arkansas Ave > >Bennett, CO 80102 > >H 303-644-4538 > >C 720-281-5406 > >prueg...@hotmail.com > > > > > > > >> From: r...@psu.edu > >> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu > >> Date: Sat, 3 Jan 2015 23:15:33 +0000 > >> Subject: RE: [Histonet] cutting honey bees > >> CC: > >> > >> I sectioned and stained honey bee and yellow jacket stingers years ago. > >> They wanted to show the difference between the stingers. I wasn't sure > >>what to do so I processed and handled like everything else. I was able > >>to get some good sections. I put 6 stingers in each block and cut > >>several sections figuring there should be at least one good stinger in > >>each block and it worked. > >> Roberta Horner > >> Penn State University > >> Animal Diagnostic Lab > >> ________________________________________ > >> From: histonet-boun...@lists.utsouthwestern.edu > >> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg > >> [classic...@gmail.com] > >> Sent: Saturday, January 03, 2015 6:08 PM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] cutting honey bees > >> > >> Has anyone had experience embedding and cutting honey bees. I am sure > >> there are some issues with the harder exoskeleton. Would that have to > >> be dissected away first. I am considering helping a student with a > >> science fair project on bees. > >> > >> Douglas Gregg > >> Veterianary pathologist > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet