Re: [Histonet] Cell block processing
There's been excellent discussion on fixation, IHC and cells blocks, with Joe Walker's email summing up the situation nicely. For those interested in side by side comparison of IHC staining of cytology cell blocks using different fixatives go to http://cpqa.ca/main/wp-content/uploads/2014/06/2014-Thomson.pdf Regards John Garratt www.ciqc.ca ‐‐‐ Original Message ‐‐‐ On Monday, October 28, 2019 7:38 AM, Joe W. Walker, Jr. via Histonet wrote: > Hi Terri, > > At one time we did the same thing but have changed our approach in light of > the FDA's and CAP's view point on ASRs. The potential problem is that IHCs > are all validated/tested by the manufacturer on FFPE tissue. By introducing > methanol/ethanol as the first step in fixation, you potentially have altered > the initial fixation steps. I've attended several meetings on this topic and > have been advised to stop performing IHC on methanol/ethanol fixed specimens > unless we validated that this fixation step doesn't alter the expression of > the target antigen in the tissue. Formalin fixation after an alcohol fixation > doesn't change/reverse any alterations to the antigen in the tissue. > > We utilize an IBF tissue fixative but have also validated this fixative with > our antibody panels that we offer. The IBF does contain a small amount of > alcohol and the fixative is slightly different than 10% buffered formalin. > > I agree that CytoLyt is excellent at lysing red blood cells but would just > caution you on using the specimen for IHC without a disclaimer within your > report or validating your IHCs on these specimens to ensure they work as > expected. Keep in mind that most control tissue is FFPE and using it to > compare if the IHC worked in a first fixed alcohol specimen is not an apples > to apples comparison. > > Cheers, > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology Manager > joewal...@rrmc.org, www.rrmc.org > > -Original Message- > From: Terri Braud via Histonet histonet@lists.utsouthwestern.edu > Sent: Monday, October 28, 2019 10:14 AM > To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Cell block processing > > [External Email] This email originated from outside of the organization. > Think before you click: Don’t click on links, open attachments or respond to > requests for sensitive information if the email looks suspicious or you don’t > recognize the sender. > > We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn > fluids, but then we fix the cell block "pellet" in formalin. > We have had no problems with immunos, and are able to lyse the RBCs to > provide a nice, clear specimen. > Hope this helps. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rHP2_dFONxwRmR87ddavejT_o6RIcXBah8jw7nMUO0tWdC5KpksUnk2bttyIMkU%24=02|01|jwwalker%40rrmc.org|eeac9b3afe444de27cd208d75bb1634b|0e55647d438e4a448437e959c3cf2240|0|0|637078689748526729=1%2BpvxH9TcvKxAo6jgjr7fobCAUUz35sHbsLfoBvHmGE%3D=0 > [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cell block processing
Hi Terri, At one time we did the same thing but have changed our approach in light of the FDA's and CAP's view point on ASRs. The potential problem is that IHCs are all validated/tested by the manufacturer on FFPE tissue. By introducing methanol/ethanol as the first step in fixation, you potentially have altered the initial fixation steps. I've attended several meetings on this topic and have been advised to stop performing IHC on methanol/ethanol fixed specimens unless we validated that this fixation step doesn't alter the expression of the target antigen in the tissue. Formalin fixation after an alcohol fixation doesn't change/reverse any alterations to the antigen in the tissue. We utilize an IBF tissue fixative but have also validated this fixative with our antibody panels that we offer. The IBF does contain a small amount of alcohol and the fixative is slightly different than 10% buffered formalin. I agree that CytoLyt is excellent at lysing red blood cells but would just caution you on using the specimen for IHC without a disclaimer within your report or validating your IHCs on these specimens to ensure they work as expected. Keep in mind that most control tissue is FFPE and using it to compare if the IHC worked in a first fixed alcohol specimen is not an apples to apples comparison. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewal...@rrmc.org, www.rrmc.org -Original Message- From: Terri Braud via Histonet Sent: Monday, October 28, 2019 10:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids, but then we fix the cell block "pellet" in formalin. We have had no problems with immunos, and are able to lyse the RBCs to provide a nice, clear specimen. Hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rHP2_dFONxwRmR87ddavejT_o6RIcXBah8jw7nMUO0tWdC5KpksUnk2bttyIMkU%24data=02%7C01%7Cjwwalker%40rrmc.org%7Ceeac9b3afe444de27cd208d75bb1634b%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637078689748526729sdata=1%2BpvxH9TcvKxAo6jgjr7fobCAUUz35sHbsLfoBvHmGE%3Dreserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cell block processing
We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids, but then we fix the cell block "pellet" in formalin. We have had no problems with immunos, and are able to lyse the RBCs to provide a nice, clear specimen. Hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cell block processing
Hi Charles, I have had excellent success with lysing the red blood cells (using Isotonic Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma. The lysing solution contains EDTA so you will need to add a few drops of 1% calcium chloride. Method as follows: Lysis solution Ammonium Chloride4.5g Potassium carbonate 0.5g EDTA 0.0186g Distilled water 500mls Method: 1. Centrifuge bloody fluid. 2. Remove supernatant and add equal volume of lysis solution. 3. Resuspend and incubate for 5 minutes at 4oC. 4. Centrifuge, if blood still remains, then repeat from step 2. 5. Rinse in Hanks or RPMI, centrifuge. 6. Mix pellet in a few drops of plasma. 7. Add thromboplastin and a few drops of 1% Calcium Chloride, mix gently and allow clot to form. 8. Add 10% buffered formalin and fix and process as usual. Reference: Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479 The lysis solution can also be purchased commercially from several companies (eg Biolegend). It is commonly used for sample preparation for flow cytometry. Check the SDS to make sure it does not contain formaldehyde. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Charles Riley via Histonet Sent: Friday, 25 October 2019 23:12 To: Histo List Subject: [Histonet] Cell block processing Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens?Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cell block processing
I agree with Joe. We used to use ETOH for cell blocks, but stopped using it when we started doing IHC biomarker testing on these specimens. Alcohol is good for some proteomic targets, but can be a disaster for others. We also fix all of our cell block specimens that are collected in saline or RPMI in formalin. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -Original Message- From: Joe W. Walker, Jr. via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, October 25, 2019 4:36 PM To: Charles Riley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing EXTERNAL email is from outside HHC. DO NOT open attachments or click links from unknown senders. As a cytotech, that wouldn’t be my first choice for collections and FNA specimens. The main reason is that once fixed in 95% ETOH you are limited if you need to perform IHC stains on the cell block unless you have validated your IHCs on ETOH fixed specimens. How do you process the FNA rinses that in in ETOH: Only Cell blocks or do you have another cytology liquid prep? Without knowing your prep process, I’d suggest collecting the FNA needle rinses in Hank’s Balanced Salt solution. After making the cell block, you could then formalin fix them. I can send you a procedure that we utilize for this process. The cell blocks cut great, look great, and you can perform IHC an molecular testing if needed. Joe Walker From: Charles Riley Sent: Friday, October 25, 2019 12:57 PM To: Joe W. Walker, Jr. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. mailto:jwwal...@rrmc.org>> wrote: Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewal...@rrmc.org<mailto:joewal...@rrmc.org>, https://urldefense.proofpoint.com/v2/url?u=http-3A__www.rrmc.org=DwIGaQ=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA=JIavq_Wfz4S4TppCsMv8kQfnmD9wLQhRVNqSm1eJAO4= <https://urldefense.proofpoint.com/v2/url?u=https-3A__nam02.safelinks.protection.outlook.com_-3Furl-3Dhttp-253A-252F-252Fwww.rrmc.org-26data-3D02-257C01-257Cjwwalker-2540rrmc.org-257C2403b15e19aa486985ac08d7596c3f45-257C0e55647d438e4a448437e959c3cf2240-257C0-257C0-257C637076193772807309-26sdata-3DVxp3O6jX4PVU4FDmXfFTwIQFG0oR03V3csN45z012Mg-253D-26reserved-3D0=DwIGaQ=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA=Vn_yzz1gfScLUDaT695jKQgsKf5KhNHP41Y1ZT_Vz-Y= > -Original Message- From: Charles Riley via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: Friday, October 25, 2019 8:13 AM To: Histo List mailto:histonet@lists.utsouthwestern.edu>> Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens?Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> https://urldefense.proofpoint.com/v2/url?u=https-3A__nam02.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.com-252Fv3-252F-5F-5Fhttp-253A-252F-252Flists.utsouthwestern.edu-252Fmailman-252Flistinfo-252Fhistonet-5F-5F-253B-215JlSGkcjda6Eqs5J-21rntZr6E7tVvCc2tzoJy-5FL8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY-2524-26amp-3Bdata-3D02-257C01-257Cjwwalker-2540rrmc.org-257C6e4cdd79edfa4e1ef23408d75944c458-257C0e55647d438e4a448437e959c3cf2240-257C0-257C0-257C637076024216531651-26amp-3Bsdata-3DxAxqyyCfRepB3DlAl-252Fw651nk3B5ViHQLjqdToa2iAhw-253D-26amp-3Breserved-3D0=DwIGaQ=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=2Cd
Re: [Histonet] Cell block processing
As a cytotech, that wouldn’t be my first choice for collections and FNA specimens. The main reason is that once fixed in 95% ETOH you are limited if you need to perform IHC stains on the cell block unless you have validated your IHCs on ETOH fixed specimens. How do you process the FNA rinses that in in ETOH: Only Cell blocks or do you have another cytology liquid prep? Without knowing your prep process, I’d suggest collecting the FNA needle rinses in Hank’s Balanced Salt solution. After making the cell block, you could then formalin fix them. I can send you a procedure that we utilize for this process. The cell blocks cut great, look great, and you can perform IHC an molecular testing if needed. Joe Walker From: Charles Riley Sent: Friday, October 25, 2019 12:57 PM To: Joe W. Walker, Jr. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. mailto:jwwal...@rrmc.org>> wrote: Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewal...@rrmc.org<mailto:joewal...@rrmc.org>, www.rrmc.org<https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.rrmc.org=02%7C01%7Cjwwalker%40rrmc.org%7C2403b15e19aa486985ac08d7596c3f45%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076193772807309=Vxp3O6jX4PVU4FDmXfFTwIQFG0oR03V3csN45z012Mg%3D=0> -Original Message- From: Charles Riley via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: Friday, October 25, 2019 8:13 AM To: Histo List mailto:histonet@lists.utsouthwestern.edu>> Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens?Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY%24data=02%7C01%7Cjwwalker%40rrmc.org%7C6e4cdd79edfa4e1ef23408d75944c458%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076024216531651sdata=xAxqyyCfRepB3DlAl%2Fw651nk3B5ViHQLjqdToa2iAhw%3Dreserved=0<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__https%3A%2F%2Fnam02.safelinks.protection.outlook.com%2F%3Furl%3Dhttps*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__*3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY*24%26data%3D02*7C01*7Cjwwalker*40rrmc.org*7C6e4cdd79edfa4e1ef23408d75944c458*7C0e55647d438e4a448437e959c3cf2240*7C0*7C0*7C637076024216531651%26sdata%3DxAxqyyCfRepB3DlAl*2Fw651nk3B5ViHQLjqdToa2iAhw*3D%26reserved%3D0__%3BJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!5JlSGkcjda6Eqs5J!rGVYY5dOQ2xRCn-bsaKkh1bKH2qKCaDhf7LTZHmhPvEE8IgM-u6EOhcj-0W-HWE%24=02%7C01%7Cjwwalker%40rrmc.org%7C2403b15e19aa486985ac08d7596c3f45%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076193772817305=bd7V2hr4xJ0fe%2FUs39UFEPQaB4RKHS45Y42KOAaimIs%3D=0> [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.rrmc.org%2Fapp%2Ffiles%2Fpublic%2F2633%2F2019_hyht_sig-_jan2019_final.jpg=02%7C01%7Cjwwalker%40rrmc.org%7C2403b15e19aa486985ac08d7596c3f45%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076193772817305=jp9HasHg%2BWY50DYjwo20Rv2X46JxVh3M6Oshnp%2B44H0%3D=0>] -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cell block processing
Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. wrote: > Hi Charles, > > What are you collecting the FNA into? Cytorich? Cytolyt? Other? > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology Manager > joewal...@rrmc.org, www.rrmc.org > > -Original Message- > From: Charles Riley via Histonet > Sent: Friday, October 25, 2019 8:13 AM > To: Histo List > Subject: [Histonet] Cell block processing > > [External Email] This email originated from outside of the organization. > Think before you click: Don’t click on links, open attachments or respond > to requests for sensitive information if the email looks suspicious or you > don’t recognize the sender. > > > Does anyone have any tips or suggestions on how to better process extremely > bloody FNA specimens?Is there anyway to clear out some or all of the > blood without destroying the other tissues? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY%24data=02%7C01%7Cjwwalker%40rrmc.org%7C6e4cdd79edfa4e1ef23408d75944c458%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076024216531651sdata=xAxqyyCfRepB3DlAl%2Fw651nk3B5ViHQLjqdToa2iAhw%3Dreserved=0 > [ > https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg > ] > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cell block processing
Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewal...@rrmc.org, www.rrmc.org -Original Message- From: Charles Riley via Histonet Sent: Friday, October 25, 2019 8:13 AM To: Histo List Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens?Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY%24data=02%7C01%7Cjwwalker%40rrmc.org%7C6e4cdd79edfa4e1ef23408d75944c458%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076024216531651sdata=xAxqyyCfRepB3DlAl%2Fw651nk3B5ViHQLjqdToa2iAhw%3Dreserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
