Re: [Histonet] Delay in embedding
We do it all the time. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, December 18, 2019 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 193, Issue 12 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Delay in embedding? (P Sicurello) 2. Re: Delay in embedding? (Theresa Dalton) 3. Re: Delay in embedding? (Tony Henwood (SCHN)) 4. Re: Delay in embedding? (Jennifer Saunders) 5. Re: Delay in embedding? (Garrey Faller) 6. Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy! (Pam Barker) 7. plural fluid prep (Michelle Jamison) -- Message: 1 Date: Tue, 17 Dec 2019 11:30:58 -0800 From: P Sicurello To: HistoNet Subject: [Histonet] Delay in embedding? Message-ID: Content-Type: text/plain; charset="UTF-8" Good Morning Listers, How many out there will process tissue and then leave the cassettes at room temperature and embed it at a later time (hours or the next day)? Please send me your opinions about doing this. I think it?s a bad idea, others I speak with disagree. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. -- Message: 2 Date: Tue, 17 Dec 2019 15:33:43 -0500 From: Theresa Dalton To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? Message-ID: <693b4d62-df63-486c-9017-d24b9f190...@icloud.com> Content-Type: text/plain; charset=utf-8 We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at room > temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad idea, > others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 17 Dec 2019 22:28:13 +0000 From: "Tony Henwood (SCHN)" To: Theresa Dalton , P Sicurello Cc: "'histonet@lists.utsouthwestern.edu'" Subject: Re: [Histonet] Delay in embedding? Message-ID: Content-Type: text/plain; charset="utf-8" Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so thes
Re: [Histonet] Delay in embedding?
I agree with replies/statements 3, 4, 5 Anyone who states differently have not experienced this contingency. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Delay in embedding?
I’ve done it a handful of times when my only Histotech called in sick. No effect on quality. Garrey Sent from my iPhone > On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet > wrote: > > I don’t see a problem with it. We all melt and re-embed tissue for all kinds > of reasons every day. I think it is much better for the tissue than keeping > it warm for an extended period of time. Jen Saunders > > > Sent from Yahoo Mail for iPhone > > > On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet > wrote: > > Hi Paula, > > We routinely do this, especially for our fetal autopsy blocks. > We are then able to process and let them set at room temp until we are able > to embed and cut. > Some cases are more urgent than others so these can be expedited a lot easier > since they will only need embedding, sectioning and staining. We also get > best usage of our limited processing capabilities. > It is more efficient for us. > > Morphology, staining and immunohistochemistry is not affected. > > It is better to do this rather than leave them at 64oC plus for extended > times prior to embedding since many antigens will be adversely affected. > > Take home point - do not overcook your tissues. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children’s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -Original Message- > From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, 18 December 2019 7:34 AM > To: P Sicurello > Cc: HistoNet > Subject: Re: [Histonet] Delay in embedding? > > We have done this - only on an emergency basis. > > Sent from my iPhone > >> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet >> wrote: >> >> Good Morning Listers, >> >> >> >> How many out there will process tissue and then leave the cassettes at >> room temperature and embed it at a later time (hours or the next day)? >> >> >> >> Please send me your opinions about doing this. I think it’s a bad >> idea, others I speak with disagree. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 9300 Campus Point Drive >> >> La Jolla, CA 92037 >> (P): 858-249-5610 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited. If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Delay in embedding?
I don’t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time. Jen Saunders Sent from Yahoo Mail for iPhone On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet wrote: Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > wrote: > > Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it’s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Delay in embedding?
Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > wrote: > > Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it’s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Delay in embedding?
We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > wrote: > > Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at room > temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it’s a bad idea, > others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
