Re: [Histonet] HT HistoDeck question...

2013-10-07 Thread Kim Donadio 
I think the problem with "a" is that 37% formaldehyde is what is considered 
100% formalin not 10% nbf. So for all technical purposes I've never known of 
anyone fixing samples in 100% formalin either But I would agree it depends on 
what your doing with the sample if it got acetone or not. So i agree with you 
if they  are asking what the fixative is for the fs to go for perm then they 
need to clarify and fix what they are saying for option "a". Im not sure but it 
seems like they are trying to pass 37% formaldehyde off as 10% formalin. Anyone 
else get that feeling? 

Sent from my iPhone

On Oct 3, 2013, at 10:39 AM, "McKenzie, Emily"  wrote:

> Honestly I think this all depends on what they are actually asking. Are they 
> asking what you fix the tissue in after the frozen is complete and you need 
> to submit the remaining tissue for routine processing? If so, then A is the 
> correct answer.
> Are they asking what you fix the tissue to the slide with to stain the frozen 
> section for immediate diagnosis? If so, then the answer can be several 
> possibilities depending on what you are going to be staining/ the process you 
> will be staining with.
> I have worked in several different hospitals and each have a different 
> protocol for there frozen section staining. In order to fix the tissue to the 
> slide I have used 95% alcohol and acidic acid alcohol. The only time I have 
> used formaldehyde is when I use the vapors for fat staining.
> I think this might be a question to take up with ASCP. They are the ones 
> administering the test.
> 
> Emily K. McKenzie BS, HT(ASCP)
> 
> Memorial Medical Center│701 North First Street│Springfield, IL 62781
> Ph: 217-788-3991│email: mckenzie.em...@mhsil.com
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy 
> Wenk
> Sent: Thursday, October 03, 2013 9:22 AM
> To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] HT HistoDeck question...
> 
> I agree, there is probably more than one correct answer to this question, 
> depending upon whether you are planning on doing stains for lipids, IHC, 
> immunofluoresence or muscle enzymes.
> 
> But I don't think (A) full strength 37-40% formaldehyde solution would ever 
> be the correct answer. Unless you put a gauze in the bottom on the coplin 
> jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin 
> jar, and fix the section in formaldehyde vapors. But the question does say 
> SOLUTION, not VAPOR. So I still think A is wrong. And most likely full 
> strength acetic acid (C) is wrong - would eat the tissue off the slide.
> 
> That leaves cold acetone (B) , which is good for some antibodies and some 
> enzymes, or alcoholic formalin (D) which might be OK, but most of the time 
> things either like alcohol and hate formalin, or they like formalin and don't 
> like alcohol. So I would think most FS that we want to fix would not 
> particularly like alcoholic formalin.
> 
> And none of the solutions listed are good for lipids.
> 
> So, given the question (with incomplete information) and the choices of 
> answers, I would still side with (B) cold acetone.
> 
> Now - a little aside - for the questions on the ASCP HT and HTL exams - if it 
> is a new question, the people on the HT/HTL exam committee would be looking 
> at it intensely before it goes on the exam for the first time. If the 
> committee people are having problems answering it (like we are here), the 
> question would be reworked until all the issues are resolved (such as putting 
> in "for lymph node IHC" into the question). If it makes it past the 
> committee, and the stats from the exam show that many people are having 
> problems answering it, the question is pulled from the exam and is not used 
> in the person's score. The question is then sent back to the HT/HTL exam 
> committee, to try to figure out why examinees were having problems, and the 
> question reworked again.
> 
> As someone who has written exam questions at the school for 20+ years, I can 
> tell you that it really is hard to write exam questions. I think I've covered 
> everything in the question so that it is straight forward, and then half the 
> students read something into the question that I never thought of, or come up 
> with a written answer that I never considered. So I either have to throw out 
> the question or give the point to the student, depending upon what's going 
> on. And then mark the question for a re-write next year.  And that doesn't 
> include me marking the wrong answer on my master sheet. It happens!
> 
&

RE: [Histonet] HT HistoDeck question...

2013-10-03 Thread Jennifer MacDonald 
the protocol calls for 1 minute in the 40% formaldehyde and then rinse the 
sections well.



From:   "Rathborne, Toni" 
To: "'Jennifer MacDonald'" , "Grantham, Andrea L 
-   (algranth)" 
Cc: HISTONET , 
"histonet-boun...@lists.utsouthwestern.edu" 

Date:   10/03/2013 11:42 AM
Subject:RE: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



For those of you who use the 40% formaldehyde, how long is you fixation 
time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes 
it gets hectic if you have multiple frozens all at once. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure 
the author of the question had something in mind and other options didn't 
occur to him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" 
To: 
Cc: HISTONET 
Date:   10/03/2013 08:33 AM
Subject:Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with 
the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use 
almost daily combined with some steps from PolyScientific's ORO protocol), 
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the 
slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or 
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu<mailto:algra...@email.arizona.edu>
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] HT HistoDeck question...

2013-10-03 Thread Benjamin 
It would dissolve the fat if you used acetone wouldnt it? Without knowing the 
tissue type or stain, the answer is A.  All other choices dissolve fat I think. 
Good luck on your exam! 

Sent from my iPhone

On Oct 3, 2013, at 8:15 AM, "Lee & Peggy Wenk"  wrote:

> Personally, I think it's "a" is a wrong answer, and that you are correct that 
> "b" is a better answer. My students and I have found a couple of other 
> questions that we thought had the wrong answer indicated in the study set.
> 
> Peggy A. Wenk, HTL(ASCP)SLS
> -Original Message- From: Stephenson, Sheryl
> Sent: Thursday, October 03, 2013 7:21 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HT HistoDeck question...
> 
> Hi,
> Please clarify why this answer to the HistoDeck study question is  a) and not 
> b).
> 
> Here is the question:
> 
> 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally 
> fixed in which of the following solutions?
> a) 37%-40% formaldehyde
> b) Cold acetone
> c) Acetic acid alcohol
> d) Alcoholic formalin
> 
> Thanks,
> 
> 
> Sheryl Stephenson | Histology Technician
> 
> 
> 
> 
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RE: [Histonet] HT HistoDeck question...

2013-10-03 Thread Rathborne, Toni 
For those of you who use the 40% formaldehyde, how long is you fixation time on 
frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets 
hectic if you have multiple frozens all at once. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure the 
author of the question had something in mind and other options didn't occur to 
him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" 
To: 
Cc: HISTONET 
Date:   10/03/2013 08:33 AM
Subject:    Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with the 
sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use almost 
daily combined with some steps from PolyScientific's ORO protocol), step #1 
says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in 
the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 40% 
formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or alcohol 
this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu<mailto:algra...@email.arizona.edu>
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] HT HistoDeck question...

2013-10-03 Thread Jennifer MacDonald 
We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure 
the author of the question had something in mind and other options didn't 
occur to him/her at the time.
Jennifer




From:   "Grantham, Andrea L - (algranth)" 
To: 
Cc: HISTONET 
Date:   10/03/2013 08:33 AM
Subject:    Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



I'd go with "A", but it really depends on what you are going to do with 
the sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use 
almost daily combined with some steps from PolyScientific's ORO protocol), 
step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the 
slides in the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 
40% formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or 
alcohol this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu<mailto:algra...@email.arizona.edu>
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] HT HistoDeck question...

2013-10-03 Thread Grantham, Andrea L - (algranth) 
I'd go with "A", but it really depends on what you are going to do with the 
sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use almost 
daily combined with some steps from PolyScientific's ORO protocol), step #1 
says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in 
the liquid 40%.
I have fixed frozen sections for regular H&E and other stains as well in 40% 
formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or alcohol 
this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] HT HistoDeck question...

2013-10-03 Thread McKenzie, Emily 
Honestly I think this all depends on what they are actually asking. Are they 
asking what you fix the tissue in after the frozen is complete and you need to 
submit the remaining tissue for routine processing? If so, then A is the 
correct answer.
Are they asking what you fix the tissue to the slide with to stain the frozen 
section for immediate diagnosis? If so, then the answer can be several 
possibilities depending on what you are going to be staining/ the process you 
will be staining with.
I have worked in several different hospitals and each have a different protocol 
for there frozen section staining. In order to fix the tissue to the slide I 
have used 95% alcohol and acidic acid alcohol. The only time I have used 
formaldehyde is when I use the vapors for fat staining.
I think this might be a question to take up with ASCP. They are the ones 
administering the test.

Emily K. McKenzie BS, HT(ASCP)

Memorial Medical Center│701 North First Street│Springfield, IL 62781
Ph: 217-788-3991│email: mckenzie.em...@mhsil.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
Sent: Thursday, October 03, 2013 9:22 AM
To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

I agree, there is probably more than one correct answer to this question, 
depending upon whether you are planning on doing stains for lipids, IHC, 
immunofluoresence or muscle enzymes.

But I don't think (A) full strength 37-40% formaldehyde solution would ever be 
the correct answer. Unless you put a gauze in the bottom on the coplin jar, 
pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and 
fix the section in formaldehyde vapors. But the question does say SOLUTION, not 
VAPOR. So I still think A is wrong. And most likely full strength acetic acid 
(C) is wrong - would eat the tissue off the slide.

That leaves cold acetone (B) , which is good for some antibodies and some 
enzymes, or alcoholic formalin (D) which might be OK, but most of the time 
things either like alcohol and hate formalin, or they like formalin and don't 
like alcohol. So I would think most FS that we want to fix would not 
particularly like alcoholic formalin.

And none of the solutions listed are good for lipids.

So, given the question (with incomplete information) and the choices of 
answers, I would still side with (B) cold acetone.

Now - a little aside - for the questions on the ASCP HT and HTL exams - if it 
is a new question, the people on the HT/HTL exam committee would be looking at 
it intensely before it goes on the exam for the first time. If the committee 
people are having problems answering it (like we are here), the question would 
be reworked until all the issues are resolved (such as putting in "for lymph 
node IHC" into the question). If it makes it past the committee, and the stats 
from the exam show that many people are having problems answering it, the 
question is pulled from the exam and is not used in the person's score. The 
question is then sent back to the HT/HTL exam committee, to try to figure out 
why examinees were having problems, and the question reworked again.

As someone who has written exam questions at the school for 20+ years, I can 
tell you that it really is hard to write exam questions. I think I've covered 
everything in the question so that it is straight forward, and then half the 
students read something into the question that I never thought of, or come up 
with a written answer that I never considered. So I either have to throw out 
the question or give the point to the student, depending upon what's going on. 
And then mark the question for a re-write next year.  And that doesn't include 
me marking the wrong answer on my master sheet. It happens!

Peggy A. Wenk, HTL(ASCP)SLS

-Original Message-
From: Watson, Linda
Sent: Thursday, October 03, 2013 9:40 AM
To: Lee & Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...

For frozen cut sections, would the fixation also depend on what you plan on 
doing with it. For example, H&E, Special Stain or IHC? Please correct if I am 
wrong. I think that is a trick question!!!

>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-
>boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
>Sent: Thursday, October 03, 2013 8:16 AM
>To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] HT HistoDeck question...
>
>Personally, I think it's "a" is a wrong answer, and that you are
>correct that "b" is a better answer. My students and I have found a
>couple of other questions that we thought had the wrong answer
>indicated in t

Re: [Histonet] HT HistoDeck question...

2013-10-03 Thread Lee & Peggy Wenk 
I agree, there is probably more than one correct answer to this question, 
depending upon whether you are planning on doing stains for lipids, IHC, 
immunofluoresence or muscle enzymes.


But I don't think (A) full strength 37-40% formaldehyde solution would ever 
be the correct answer. Unless you put a gauze in the bottom on the coplin 
jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin 
jar, and fix the section in formaldehyde vapors. But the question does say 
SOLUTION, not VAPOR. So I still think A is wrong. And most likely full 
strength acetic acid (C) is wrong - would eat the tissue off the slide.


That leaves cold acetone (B) , which is good for some antibodies and some 
enzymes, or alcoholic formalin (D) which might be OK, but most of the time 
things either like alcohol and hate formalin, or they like formalin and 
don't like alcohol. So I would think most FS that we want to fix would not 
particularly like alcoholic formalin.


And none of the solutions listed are good for lipids.

So, given the question (with incomplete information) and the choices of 
answers, I would still side with (B) cold acetone.


Now - a little aside - for the questions on the ASCP HT and HTL exams - if 
it is a new question, the people on the HT/HTL exam committee would be 
looking at it intensely before it goes on the exam for the first time. If 
the committee people are having problems answering it (like we are here), 
the question would be reworked until all the issues are resolved (such as 
putting in "for lymph node IHC" into the question). If it makes it past the 
committee, and the stats from the exam show that many people are having 
problems answering it, the question is pulled from the exam and is not used 
in the person's score. The question is then sent back to the HT/HTL exam 
committee, to try to figure out why examinees were having problems, and the 
question reworked again.


As someone who has written exam questions at the school for 20+ years, I can 
tell you that it really is hard to write exam questions. I think I've 
covered everything in the question so that it is straight forward, and then 
half the students read something into the question that I never thought of, 
or come up with a written answer that I never considered. So I either have 
to throw out the question or give the point to the student, depending upon 
what's going on. And then mark the question for a re-write next year.  And 
that doesn't include me marking the wrong answer on my master sheet. It 
happens!


Peggy A. Wenk, HTL(ASCP)SLS

-Original Message- 
From: Watson, Linda

Sent: Thursday, October 03, 2013 9:40 AM
To: Lee & Peggy Wenk ; Stephenson, Sheryl ; 
histonet@lists.utsouthwestern.edu

Subject: RE: [Histonet] HT HistoDeck question...

For frozen cut sections, would the fixation also depend on what you plan on 
doing with it. For example, H&E, Special Stain or IHC? Please correct if I 
am wrong. I think that is a trick question!!!



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-
boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
Sent: Thursday, October 03, 2013 8:16 AM
To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

Personally, I think it's "a" is a wrong answer, and that you are correct
that "b" is a better answer. My students and I have found a couple of
other
questions that we thought had the wrong answer indicated in the study
set.

Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Stephenson, Sheryl
Sent: Thursday, October 03, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT HistoDeck question...

Hi,
Please clarify why this answer to the HistoDeck study question is  a)
and
not b).

Here is the question:

 'Frozen section slides cut from fresh, unfixed tissue specimens are
optimally fixed in which of the following solutions?
a) 37%-40% formaldehyde
b) Cold acetone
c) Acetic acid alcohol
d) Alcoholic formalin

Thanks,


Sheryl Stephenson | Histology Technician




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immed

RE: [Histonet] HT HistoDeck question...

2013-10-03 Thread Stephenson, Sheryl 
Thank you All!

Sheryl Stephenson | Histology Technician 



 

-Original Message-
From: Bernice Frederick [mailto:b-freder...@northwestern.edu] 
Sent: Thursday, October 03, 2013 9:43 AM
To: Watson, Linda; Lee & Peggy Wenk; Stephenson, Sheryl; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...

We fix H&E's in 95%  and our IHC protocol is acetone/alcohol fixation.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda
Sent: Thursday, October 03, 2013 8:40 AM
To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...

For frozen cut sections, would the fixation also depend on what you plan on 
doing with it. For example, H&E, Special Stain or IHC? Please correct if I am 
wrong. I think that is a trick question!!!

>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- 
>boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
>Sent: Thursday, October 03, 2013 8:16 AM
>To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] HT HistoDeck question...
>
>Personally, I think it's "a" is a wrong answer, and that you are 
>correct that "b" is a better answer. My students and I have found a 
>couple of other questions that we thought had the wrong answer 
>indicated in the study set.
>
>Peggy A. Wenk, HTL(ASCP)SLS
>-Original Message-
>From: Stephenson, Sheryl
>Sent: Thursday, October 03, 2013 7:21 AM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] HT HistoDeck question...
>
>Hi,
>Please clarify why this answer to the HistoDeck study question is  a) 
>and not b).
>
>Here is the question:
>
>  'Frozen section slides cut from fresh, unfixed tissue specimens are 
>optimally fixed in which of the following solutions?
>a) 37%-40% formaldehyde
>b) Cold acetone
>c) Acetic acid alcohol
>d) Alcoholic formalin
>
>Thanks,
>
>
>Sheryl Stephenson | Histology Technician
>
>
>
>
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RE: [Histonet] HT HistoDeck question...

2013-10-03 Thread Bernice Frederick 
We fix H&E's in 95%  and our IHC protocol is acetone/alcohol fixation.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda
Sent: Thursday, October 03, 2013 8:40 AM
To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...

For frozen cut sections, would the fixation also depend on what you plan on 
doing with it. For example, H&E, Special Stain or IHC? Please correct if I am 
wrong. I think that is a trick question!!!

>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- 
>boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
>Sent: Thursday, October 03, 2013 8:16 AM
>To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] HT HistoDeck question...
>
>Personally, I think it's "a" is a wrong answer, and that you are 
>correct that "b" is a better answer. My students and I have found a 
>couple of other questions that we thought had the wrong answer 
>indicated in the study set.
>
>Peggy A. Wenk, HTL(ASCP)SLS
>-Original Message-
>From: Stephenson, Sheryl
>Sent: Thursday, October 03, 2013 7:21 AM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] HT HistoDeck question...
>
>Hi,
>Please clarify why this answer to the HistoDeck study question is  a) 
>and not b).
>
>Here is the question:
>
>  'Frozen section slides cut from fresh, unfixed tissue specimens are 
>optimally fixed in which of the following solutions?
>a) 37%-40% formaldehyde
>b) Cold acetone
>c) Acetic acid alcohol
>d) Alcoholic formalin
>
>Thanks,
>
>
>Sheryl Stephenson | Histology Technician
>
>
>
>
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RE: [Histonet] HT HistoDeck question...

2013-10-03 Thread Watson, Linda 
For frozen cut sections, would the fixation also depend on what you plan on 
doing with it. For example, H&E, Special Stain or IHC? Please correct if I am 
wrong. I think that is a trick question!!!

>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-
>boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
>Sent: Thursday, October 03, 2013 8:16 AM
>To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] HT HistoDeck question...
>
>Personally, I think it's "a" is a wrong answer, and that you are correct
>that "b" is a better answer. My students and I have found a couple of
>other
>questions that we thought had the wrong answer indicated in the study
>set.
>
>Peggy A. Wenk, HTL(ASCP)SLS
>-Original Message-
>From: Stephenson, Sheryl
>Sent: Thursday, October 03, 2013 7:21 AM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] HT HistoDeck question...
>
>Hi,
>Please clarify why this answer to the HistoDeck study question is  a)
>and
>not b).
>
>Here is the question:
>
>  'Frozen section slides cut from fresh, unfixed tissue specimens are
>optimally fixed in which of the following solutions?
>a) 37%-40% formaldehyde
>b) Cold acetone
>c) Acetic acid alcohol
>d) Alcoholic formalin
>
>Thanks,
>
>
>Sheryl Stephenson | Histology Technician
>
>
>
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message (including any attachments) may contain confidential, proprietary, 
privileged and/or private information.  The information is intended to be for 
the use of the individual or entity designated above.  If you are not the 
intended recipient of this message, please notify the sender immediately, and 
delete the message and any attachments.  Any disclosure, reproduction, 
distribution or other use of this message or any attachments by an individual 
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Re: [Histonet] HT HistoDeck question...

2013-10-03 Thread Lee & Peggy Wenk 
Personally, I think it's "a" is a wrong answer, and that you are correct 
that "b" is a better answer. My students and I have found a couple of other 
questions that we thought had the wrong answer indicated in the study set.


Peggy A. Wenk, HTL(ASCP)SLS
-Original Message- 
From: Stephenson, Sheryl

Sent: Thursday, October 03, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT HistoDeck question...

Hi,
Please clarify why this answer to the HistoDeck study question is  a) and 
not b).


Here is the question:

 'Frozen section slides cut from fresh, unfixed tissue specimens are 
optimally fixed in which of the following solutions?

a) 37%-40% formaldehyde
b) Cold acetone
c) Acetic acid alcohol
d) Alcoholic formalin

Thanks,


Sheryl Stephenson | Histology Technician




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