Re: [Histonet] Unstained slides precut for IHC
Hi Carrie, Over-heating of sections (and tissue blocks) for IPX is probably one of the most significant and unappreciated pre-analytical factors that can affect immunolocalisation. We closely scrutinise slides closely for overheating, especially those sent to us for immunostaining. We strongly prefer air-dried sections that we place on our immunostaniners (we have both a Bond and Ventana Benchmark). The following might be useful: Controlled Section Baking for Immunohistochemistry One source of poor immunostaining is overheating of tissue and sections. Several authors have reported that heated slide drying adversely affects sensitivity in immunohistochemistry (1). Therefore, some have advocated the use of lower temperature drying using adhesive-coated slides to improve the sensitivity of the test (2-5). In one study (1), half the antigens were adversely affected by section drying at 80oC including 5D3, CMV, S100, HMB45 and CEA. Oates (4) used antisera to epithelial membrane antigen from three different companies and found that for slides dried at 58"C, staining was often paler than slides dried at room temperature or at 37°C. Low heat attachment of sections to slides can cause several issues including inadequate attachment of the tissue sections so that tissue sections may be lost during antigen recovery and/or immunostaining, the inability of some paraffins to melt well at 58oC, and the requirement of more than 1 hr before an immunohistochemical procedure may be started. It has been recommended that the most efficient protocol for mounting tissue sections to microscopic slides would be to attach the tissues overnight before applying the immunohistochemical procedure at a temperature at which all tissue mounting paraffins should melt (e.g., 65oC) (6). It should be remembered that a significant dewaxing of sections occurs when slides are heated a few degrees above the melting point of the wax. Laboratory ovens seem to be variable in their ability to maintain a constant temperature with the implication that it is possible to either over-cook sections thus adversely affecting antigens or under-heat them, possibly compromising subsequent de-waxing. There is also the human element. How often are slides removed from the oven at the required time? The modern automatic immunostainers have excellent on-board slide heating to achieve reproducible, accurate antigen retrieval. This feature also allows controlled “baking” of sections and being able to programme a set time, removes the possibility of human error. At the Children’s Hospital, the Bond 3 is used for automated immunohistochemistry. A study was designed to assess the usefulness of on-board baking in routine immunohistochemistry. Control sections were immunostained for several antigens (see table) using the Bond 3 on-board baking and dewax facility. Freshly cut sections were dried at 37oC for 5 minutes to remove excess water. Slides were then loaded onto the Bond and the baking procedure used was 35 minutes at 63oC. Stained controls were compared with control slides stained prior to the instigation of the on-board bake procedure. The historic procedure involved heating sections at 63-65oC for 35 minutes in a large fan-forced dry-air oven (7). BCL-2 Mum-1 CD31 BCL-6 Calretinin SATB2 BOB-1 S100CyclinD1 CD20ALK-1 MPO CD21Ki67INI-1 CD3 SynaptophsinBRG-1 HMB-45 ChromograninInhibin Melan A MyogeninDesmin The results showed that there was no difference between controls stained with the historic compared to the on-board baking procedure except for BCL-6 which the new procedure gave stronger staining. (see figure). In conclusion, we expect that on-board baking of sections should allow laboratories to have better control over the pre-analytical variables that can adversely affect the immunohistochemistry staining. References 1. Henwood, A. F. (2005). Effect of slide drying at 80oC on immunohistochemistry. Journal of Histotechnology, 28(1), 45-46. 2. Wakins, J., Kellock, D., Gillet, C., Egan, M., Pontin, J. E., Millis, R. R., & Levinson, D. A. (1990). Enhancement of immunostaining. Histopathology, 17(2), 185-185. 3. Dodson, A., Davies, E., & Waring, J. (1991). APTES, a section adhesive for immunocytochemistry; and experiences of slide drying at room temperature. Histopathology, 19(5), 484-485. 4. Oates J. (1993) The effect of temperature on immunostaining. Br J Biomed Sci 50: 157-158, 5. Williams, J. H., Mepham, B. L., & Wright, D. H. (1997). Tissue preparation for immunocytochemistry. Journal of clinical pathology, 50(5), 422-428. 6. Jones, W. T., Stockard, C. R., & Grizzle, W. E. (2001). Effects of time and temperature during attachment of sections to microscope slides on immunohistochemical detection of antigens. Biotechnic & Histochemistry, 76(2), 55-58. 7. Henwood, A. F. (2012). The application of heated detergent dewaxing
Re: [Histonet] Unstained slides
How about frozen sections cut for immunofluorescence stored at -20? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 5, 2018 at 1:12 PM Hobbs, Carl via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > Hi > > Depends on what you mean by cryosections. > Unfixed/fixed? > Stored at RT, 4C, -20C, -80C. > Stored dry or in glycerol > > So many variables! > My opinion is to store blocks and cut sections as required. > Least variables. > Sure, one loses some tissue everytime one cuts anewa good thing. > > It IS complicated so, a project has to be thought out well in advance. > > Stimulating post > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
Hi Depends on what you mean by cryosections. Unfixed/fixed? Stored at RT, 4C, -20C, -80C. Stored dry or in glycerol So many variables! My opinion is to store blocks and cut sections as required. Least variables. Sure, one loses some tissue everytime one cuts anewa good thing. It IS complicated so, a project has to be thought out well in advance. Stimulating post Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
Hi all, It has been very interesting reading all your comments on unstained slides. This has been a forever discussion I always have everywhere I go in different research institutes. So expanding the topic, I wonder what's everyone's opinion on unstained cryosections? How long are they reliable to be used for IHC? Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E ana.malue...@baker.edu.au W www.baker.edu.au -Original Message- From: Hobbs, Carl [mailto:carl.ho...@kcl.ac.uk] Sent: Tuesday, 4 September 2018 4:54 AM To: histonet Subject: Re: [Histonet] Unstained slides I agree: cut only the sections needed. Saves space. Sure, you lose several sections of tissue when cutting more sections. That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good. However, careful organisation of exptl procedure before actual cutting will work very well. Actually, not many Ags get "oxidised"for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case) These slides are used for Yr 1 BSc practicals and are consistently positive. Nobody knows why some Ags ( and not others) lose their antigenicity, imho Oxidation is a vague reasoning. Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed specimens. Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting priv...@baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
I agree: cut only the sections needed. Saves space. Sure, you lose several sections of tissue when cutting more sections. That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good. However, careful organisation of exptl procedure before actual cutting will work very well. Actually, not many Ags get "oxidised"for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case) These slides are used for Yr 1 BSc practicals and are consistently positive. Nobody knows why some Ags ( and not others) lose their antigenicity, imho Oxidation is a vague reasoning. Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed specimens. Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
On this issue of lost of antigenicity, never forget air oxygen!René
On Monday, September 3, 2018 11:11 AM, "Frazier, John"
wrote:
Interesting that you stated that, I was at the university of Colorado
this past week and was speaking with the medical director of the
pathology department. We actually started talking about unstained
slides and their storage conditions. We actually spoke of the histonet
discussions around unstained slide storage. He stated to me that due
to the elevation and lack of humidity in Denver that the antigenicity
of unstained slides has been up to multiple years. This is due to, as
you stated, water in the tissue.
Sent from my iPad
> On Sep 3, 2018, at 9:42 AM, Cartun, Richard
> wrote:
>
> It appears that the presence of water, both endogenously and exogenously,
> plays a central role in the loss of antigenicity in stored unstained slides
> (see reference below). Labs that are experiencing significant loss of
> immunoreactivity in their unstained slides should check their tissue
> processing.
>
> Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of
> archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem
> Cytochem 2011; 59:356-365.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links
> from unknown senders.
>
> Everything has been pointed out is correct BUT also pivot on "how the
> unstained slides are kept".Kept in a box their "useful life" is quite short
> (not beyond 1 week at the most).Kept at -80ºC I have used them after years of
> being stored the principle being of deep-freezing and this is "standard
> procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil
> or paraffin covered I have used them after months of being stored the
> principle being that, isolated from air oxygen, epitopes do not oxidize
> ("weaken") of if they do, the rate is greatly slowed.On the other hand,
> usually, unstained slides are kept for only few days in the event that, lets
> say within a week, the PT decides to order some special procedure and
> sometimes it is impossible "return" to the original block many times "almost
> exhausted".Properly done storing unstained slides are extremely useful.René
>
> On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
> wrote:
>
>
> I agree with Tim as well. This is what we advise our clients to do. It takes
> some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and the 90% are are it
> takes some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and 90% are thrown away
> thrown away.
> Several laboratories that I have visited in order to reduce the amount of
> wasted tissue when refacing the blocks, is to reseal the blocks with liquid
> paraffin, that have scant or small amounts of tissue in the block, such as
> the needle core biopsy.
> Bottom line on this issue is to educate the pathologist, and not water and
> stain slides except in rare occasions
>
> Sent from my iPhone
>
>> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>>
>> I'm with Tim Morken on this one. The variability of antigenicity in storage
>> is so wide open, and there really is no recent data, so we just make a point
>> of educating our techs on not wasting tissue/levels during sectioning. If
>> the techs feel that the residual tissue in the block is in danger of being
>> exhausted, we communicate with our pathologists on how best to handle any
>> requests. Unstained slides was time, money, and storage and we are better
>> off without them.
>>
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Hun
Re: [Histonet] Unstained slides
Interesting that you stated that, I was at the university of Colorado
this past week and was speaking with the medical director of the
pathology department. We actually started talking about unstained
slides and their storage conditions. We actually spoke of the histonet
discussions around unstained slide storage. He stated to me that due
to the elevation and lack of humidity in Denver that the antigenicity
of unstained slides has been up to multiple years. This is due to, as
you stated, water in the tissue.
Sent from my iPad
> On Sep 3, 2018, at 9:42 AM, Cartun, Richard
> wrote:
>
> It appears that the presence of water, both endogenously and exogenously,
> plays a central role in the loss of antigenicity in stored unstained slides
> (see reference below). Labs that are experiencing significant loss of
> immunoreactivity in their unstained slides should check their tissue
> processing.
>
> Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of
> archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem
> Cytochem 2011; 59:356-365.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links
> from unknown senders.
>
> Everything has been pointed out is correct BUT also pivot on "how the
> unstained slides are kept".Kept in a box their "useful life" is quite short
> (not beyond 1 week at the most).Kept at -80ºC I have used them after years of
> being stored the principle being of deep-freezing and this is "standard
> procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil
> or paraffin covered I have used them after months of being stored the
> principle being that, isolated from air oxygen, epitopes do not oxidize
> ("weaken") of if they do, the rate is greatly slowed.On the other hand,
> usually, unstained slides are kept for only few days in the event that, lets
> say within a week, the PT decides to order some special procedure and
> sometimes it is impossible "return" to the original block many times "almost
> exhausted".Properly done storing unstained slides are extremely useful.René
>
>On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
> wrote:
>
>
> I agree with Tim as well. This is what we advise our clients to do. It takes
> some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and the 90% are are it
> takes some coordination with the pathologist, but it is the best strategy for
> reducing unnecessary unstained slides. In the studies that we have performed
> only 10% of the unstained slides that are cut are you and 90% are thrown away
> thrown away.
> Several laboratories that I have visited in order to reduce the amount of
> wasted tissue when refacing the blocks, is to reseal the blocks with liquid
> paraffin, that have scant or small amounts of tissue in the block, such as
> the needle core biopsy.
> Bottom line on this issue is to educate the pathologist, and not water and
> stain slides except in rare occasions
>
> Sent from my iPhone
>
>> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>>
>> I'm with Tim Morken on this one. The variability of antigenicity in storage
>> is so wide open, and there really is no recent data, so we just make a point
>> of educating our techs on not wasting tissue/levels during sectioning. If
>> the techs feel that the residual tissue in the block is in danger of being
>> exhausted, we communicate with our pathologists on how best to handle any
>> requests. Unstained slides was time, money, and storage and we are better
>> off without them.
>>
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>>
>> T
Re: [Histonet] Unstained slides
It appears that the presence of water, both endogenously and exogenously, plays
a central role in the loss of antigenicity in stored unstained slides (see
reference below). Labs that are experiencing significant loss of
immunoreactivity in their unstained slides should check their tissue processing.
Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of
archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem
Cytochem 2011; 59:356-365.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Sunday, August 19, 2018 2:09 PM
To: Frazier, John; Terri Braud
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides
This is an email from Outside HHC. USE CAUTION opening attachments or links
from unknown senders.
Everything has been pointed out is correct BUT also pivot on "how the unstained
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1
week at the most).Kept at -80ºC I have used them after years of being stored
the principle being of deep-freezing and this is "standard procedure" for IDF
"+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I
have used them after months of being stored the principle being that, isolated
from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is
greatly slowed.On the other hand, usually, unstained slides are kept for only
few days in the event that, lets say within a week, the PT decides to order
some special procedure and sometimes it is impossible "return" to the original
block many times "almost exhausted".Properly done storing unstained slides are
extremely useful.René
On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
wrote:
I agree with Tim as well. This is what we advise our clients to do. It takes
some coordination with the pathologist, but it is the best strategy for
reducing unnecessary unstained slides. In the studies that we have performed
only 10% of the unstained slides that are cut are you and the 90% are are it
takes some coordination with the pathologist, but it is the best strategy for
reducing unnecessary unstained slides. In the studies that we have performed
only 10% of the unstained slides that are cut are you and 90% are thrown away
thrown away.
Several laboratories that I have visited in order to reduce the amount of
wasted tissue when refacing the blocks, is to reseal the blocks with liquid
paraffin, that have scant or small amounts of tissue in the block, such as the
needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water and
stain slides except in rare occasions
Sent from my iPhone
> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage
> is so wide open, and there really is no recent data, so we just make a point
> of educating our techs on not wasting tissue/levels during sectioning. If
> the techs feel that the residual tissue in the block is in danger of being
> exhausted, we communicate with our pathologists on how best to handle any
> requests. Unstained slides was time, money, and storage and we are better
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
> 7. Re: Unstained slides - how long are they good for?
> (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy"
> To: P Sicurello
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had
> kept them indefinitely, then when storage was overwhelming us we reduced it
> to 2 months maximum. Now we require request for unstained to be ordered in
> the system and delivered to the pathologist. We do not hold any in the lab.
> We recut when new stains are ordered. In the past we had routinely cut extras
> "just in case" but ended up with thousands of unstained slides that were
> never used. Instead we trained everyone to reduce wastage and get good
> sections from a cut block with minimal facing. We have not stored unstained
> sections for many yea
Re: [Histonet] Unstained slides - how long are they good for?
Hi Everyone! I have seen unstained slides save a patient from re-biopsy many times. Usually it will be a case where a patient has a known diagnosis, like lung cancer. In these types of cases after diagnosis molecular testing (and IHC for PD-L1) is usually ordered. There have been countless times that I can recall where a few unstained slides on a biopsy with scant tumor was able to get us results for PD-L1, ALK FISH, and ROS1 FISH. Often in these types of a cases a touch prep can be used for Next Generation Sequencing or PCR testing like EGFR or BRAF, allowing for the full panel of molecular tests to be performed. For cases that are small specimens I would prefer to have unstained slides to fall back on for patient convenience, client satisfaction, and quicker TAT of molecular testing. Re-biopsy and re-diagnosing the new sample costs money to the patient and payers and having some unstained slides can often save those costs providing more value to the original biopsy. Sometimes when we try to save money in the lab it can result in more money being spent on healthcare overall. It is true that some antigens become more difficult to stain over time and storage is an important consideration. Limiting the production of unstained slides to small and scant needle may make storage more practical. Just some more things to consider. Sincerely, Mark Tarango On Thu, Aug 16, 2018 at 4:48 PM, P Sicurello via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
To expand on what we do at our research lab; we use 6 months as a standard maximum age of unstained slides. Also in the staining protocol for each antibodyl we have a specific shelf life for the diluted Antibody and a maximum age of unstained slides. Jamie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
I agree with Jamie Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let it be YOUR protein of interest. If you really are concerned, cut fresh sections and immunostain along with your stored sections. Imho: cut as few sections as you need. Store any unused at 4C Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
Everything has been pointed out is correct BUT also pivot on "how the unstained
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1
week at the most).Kept at -80ºC I have used them after years of being stored
the principle being of deep-freezing and this is "standard procedure" for IDF
"+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I
have used them after months of being stored the principle being that, isolated
from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is
greatly slowed.On the other hand, usually, unstained slides are kept for only
few days in the event that, lets say within a week, the PT decides to order
some special procedure and sometimes it is impossible "return" to the original
block many times "almost exhausted".Properly done storing unstained slides are
extremely useful.René
On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet"
wrote:
I agree with Tim as well. This is what we advise our clients to do. It
takes some coordination with the pathologist, but it is the best
strategy for reducing unnecessary unstained slides. In the studies
that we have performed only 10% of the unstained slides that are cut
are you and the 90% are are it takes some coordination with the
pathologist, but it is the best strategy for reducing unnecessary
unstained slides. In the studies that we have performed only 10% of
the unstained slides that are cut are you and 90% are thrown away
thrown away.
Several laboratories that I have visited in order to reduce the amount
of wasted tissue when refacing the blocks, is to reseal the blocks
with liquid paraffin, that have scant or small amounts of tissue in
the block, such as the needle core biopsy.
Bottom line on this issue is to educate the pathologist, and not water
and stain slides except in rare occasions
Sent from my iPhone
> On Aug 17, 2018, at 14:07, Terri Braud wrote:
>
> I'm with Tim Morken on this one. The variability of antigenicity in storage
> is so wide open, and there really is no recent data, so we just make a point
> of educating our techs on not wasting tissue/levels during sectioning. If
> the techs feel that the residual tissue in the block is in danger of being
> exhausted, we communicate with our pathologists on how best to handle any
> requests. Unstained slides was time, money, and storage and we are better
> off without them.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
> 7. Re: Unstained slides - how long are they good for?
> (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy"
> To: P Sicurello
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since it is variable we strive to not have unstained slides. We had
> kept them indefinitely, then when storage was overwhelming us we reduced it
> to 2 months maximum. Now we require request for unstained to be ordered in
> the system and delivered to the pathologist. We do not hold any in the lab.
> We recut when new stains are ordered. In the past we had routinely cut extras
> "just in case" but ended up with thousands of unstained slides that were
> never used. Instead we trained everyone to reduce wastage and get good
> sections from a cut block with minimal facing. We have not stored unstained
> sections for many years and they do not seem to be missed.
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -Original Message-
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, August 16, 2018 4:49 PM
> To: HistoNet
> Subject: [Histonet] Unstained slides - how long are they good for?
>
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up.. How long are *unstained* slides good for?
> Not for H&E but tests like IHC and molecular testing. These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-
Re: [Histonet] Unstained slides
I agree with Tim as well. This is what we advise our clients to do. It takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and the 90% are are it takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and 90% are thrown away thrown away. Several laboratories that I have visited in order to reduce the amount of wasted tissue when refacing the blocks, is to reseal the blocks with liquid paraffin, that have scant or small amounts of tissue in the block, such as the needle core biopsy. Bottom line on this issue is to educate the pathologist, and not water and stain slides except in rare occasions Sent from my iPhone > On Aug 17, 2018, at 14:07, Terri Braud wrote: > > I'm with Tim Morken on this one. The variability of antigenicity in storage > is so wide open, and there really is no recent data, so we just make a point > of educating our techs on not wasting tissue/levels during sectioning. If > the techs feel that the residual tissue in the block is in danger of being > exhausted, we communicate with our pathologists on how best to handle any > requests. Unstained slides was time, money, and storage and we are better > off without them. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: > 7. Re: Unstained slides - how long are they good for? > (Morken, Timothy) > > Message: 7 > Date: Fri, 17 Aug 2018 15:16:00 + > From: "Morken, Timothy" > To: P Sicurello > Subject: Re: [Histonet] Unstained slides - how long are they good for? > > > Paula, since it is variable we strive to not have unstained slides. We had > kept them indefinitely, then when storage was overwhelming us we reduced it > to 2 months maximum. Now we require request for unstained to be ordered in > the system and delivered to the pathologist. We do not hold any in the lab. > We recut when new stains are ordered. In the past we had routinely cut extras > "just in case" but ended up with thousands of unstained slides that were > never used. Instead we trained everyone to reduce wastage and get good > sections from a cut block with minimal facing. We have not stored unstained > sections for many years and they do not seem to be missed. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -Original Message- > From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, August 16, 2018 4:49 PM > To: HistoNet > Subject: [Histonet] Unstained slides - how long are they good for? > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > > Subject: Digest Footer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > > End of Histonet Digest, Vol 177, Issue 16 > * > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
I’m a histology workflow consultant that visits many AP laboratories each year. Almost every laboratory has a different retention policy. The average of most laboratories is to hold onto unstained slides for three weeks after final sign out. Typically the unstained slide can be held for a long period of time if used just for morphological staining. However if the unstained slides is going to be used for IHC or molecular testing, the antigenicity of the slide begins degrading at the point of cutting. Typically, however, for a high-quality IHC staining, if stored at room temperature, the unstained slide should not be held much longer than one month. And even at that time frame you will begin to see the degrading of the stain quality. If the slides are kept in a closed box, in refrigerator, they have longer retention. Typically up to 2 to 3 months. I hope this helps Sent from my iPhone > On Aug 16, 2018, at 19:48, P Sicurello wrote: > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning. If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests. Unstained slides was time, money, and storage and we are better off without them. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 7. Re: Unstained slides - how long are they good for? (Morken, Timothy) Message: 7 Date: Fri, 17 Aug 2018 15:16:00 + From: "Morken, Timothy" To: P Sicurello Subject: Re: [Histonet] Unstained slides - how long are they good for? Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 177, Issue 16 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides - how long are they good for?
Will definitely depend on the antibody you are using. Some references: Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059. Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. Journal of the National Cancer Institute, 89(8), 585-586. Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., Vérola, O., Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 370-374. Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(12), 943. Wester, K., Wahlund, E., Sundström, C., Ranefall, P., Bengtsson, E., Russell, P. J., ... & Busch, C. (2000). Paraffin section storage and immunohistochemistry: effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen. Applied Immunohistochemistry & Molecular Morphology, 8(1), 61-70. van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections. Applied Immunohistochemistry & Molecular Morphology, 8(4), 316-321. Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of storage-related alterations in immunoreactivity of archival tissue sections and its implications for collaborative multicenter immunohistochemical studies. Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266. Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., Dürmüller, U., Simon, R., ... & Sauter, G. (2004). Influence of slide aging on results of translational research studies using immunohistochemistry. Modern pathology, 17(11), 1414. DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term preservation of antigenicity on tissue microarrays. Laboratory investigation, 84(8), 1071. Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 13(4), 667-672. Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of prostate needle biopsies using saved interval sections vs new recut sections from the block: a prospective comparison. American journal of clinical pathology, 131(5), 683-688. Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., ... & Hewitt, S. M. (2011). Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & Cytochemistry, 59(4), 356-365. Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64. Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173. Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., & Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017. Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494. -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, 17 August 2018 9:49 AM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up.. How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information
Re: [Histonet] Unstained slides - how long are they good for?
It depends on the stability of the protein and fixation, some are stable for a week some for years. We use 6 months as a standard. Jamie On August 16, 2018 4:59:34 PM P Sicurello via Histonet wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up.. How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unstained slides
We cut a decent number of unstained as well. Mostly the tiny biopsies and cores and we do end up using most of these. I personally see no reason to cut unstained slides on anything else. Since you still have to go back and cut controls, it doesn't really save that much time but of course, it does save tissue. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, August 28, 2013 4:47 PM To: Martha Ward-Pathology Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unstained slides Martha, I have had experience in three different labs. The unstained slides can pile up quickly, especially when you are using charged slides that are "just in case" slides for sendouts, IHC, etc. For core biopsies (breasts, fine needles, prostate, etc.) we cut levels and put up at least five of the "in between sections" to keep on hand in case. We maybe use these slides 10 percent of the time and toss the rest after 3-4 weeks. It can seem wasteful, however if you have a small core, you must save precious tissue. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 9:28 AM, Martha Ward-Pathology wrote: > > > We are looking at ways to improve our work processes, save time and labor > and reduce costs, all while maintaining patient quality...as we all are of > course. > > During our conversations the subject of cutting unstained slides has come > up and we are looking for bench marking data to see if we are where we need > to be.Currently we are cutting unstained slides for various protocols > (including prostate biopsies), where the specimens are tiny, but we are > also cutting a lot of "just in case" unstained slides. Our research has > shown that about 50% of the time the unstained slides requested are never > used and we are trying to find out if that is high, low or about what other > institutions are seeing. If anyone has any data they could share with us > we would appreciate it. > > Thanks in advance for your help. I can share what I find out if others > are interested. > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f > 336.716.5890 mw...@wakehealth.edu > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained slides
Martha, I have had experience in three different labs. The unstained slides can pile up quickly, especially when you are using charged slides that are "just in case" slides for sendouts, IHC, etc. For core biopsies (breasts, fine needles, prostate, etc.) we cut levels and put up at least five of the "in between sections" to keep on hand in case. We maybe use these slides 10 percent of the time and toss the rest after 3-4 weeks. It can seem wasteful, however if you have a small core, you must save precious tissue. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 9:28 AM, Martha Ward-Pathology wrote: > > > We are looking at ways to improve our work processes, save time and labor > and reduce costs, all while maintaining patient quality...as we all are of > course. > > During our conversations the subject of cutting unstained slides has come > up and we are looking for bench marking data to see if we are where we need > to be.Currently we are cutting unstained slides for various protocols > (including prostate biopsies), where the specimens are tiny, but we are > also cutting a lot of "just in case" unstained slides. Our research has > shown that about 50% of the time the unstained slides requested are never > used and we are trying to find out if that is high, low or about what other > institutions are seeing. If anyone has any data they could share with us > we would appreciate it. > > Thanks in advance for your help. I can share what I find out if others > are interested. > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f > 336.716.5890 mw...@wakehealth.edu > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained slides
Cindy: If these outside facilities are commercial labs doing proprietary testing, many of them will pay a set fee to your laboratory for preparing the slides. This needs to be negotiated with the commercial lab rep, but may be in the $40-50 range Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, August 17, 2011 7:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] unstained slides Hello Histonetters, Recently we have seen an increase number of unstained slides requested from outside facilities. We do not release our blocks, so unstained slides are the only option for any facility to obtain our tissue. Currently we do not charge for these slides, but I am rethinking that policy. What is everyone doing about unstained slide requests? Are you charging for the slides? How much per slide? Any information would be helpful. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: Re: [Histonet] Unstained Slides
I do not bake my unstained slides for IHC controls and store them at 4dc. I bake them just before use. They do last longer this way but Richard is right, there is no telling because it all depends on the fixation, target protein stability and storage conditions. It is always a good idea to cut fresh sections if the stored unstained sections do not perform as expected. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, June 28, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; rick.garnh...@memorialhealthsystem.com Subject: SPAM-LOW: Re: [Histonet] Unstained Slides We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT. There is no set rule for stability. It all depends on fixation, the nature of the protein target, and storage conditions. I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks. We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal control, you better cut fresh sections. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 6/28/2010 11:54 AM >>> Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained Slides
Depending on the epitope you are trying to detect, the time could be limited from less than one month to 1-2 years. René J. --- On Mon, 6/28/10, rick.garnh...@memorialhealthsystem.com wrote: From: rick.garnh...@memorialhealthsystem.com Subject: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu Date: Monday, June 28, 2010, 11:54 AM Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unstained Slides
We store them in the refrigerator. And depending on the antibody they are probably good for 6 months. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rick.garnh...@memorialhealthsystem.com [rick.garnh...@memorialhealthsystem.com] Sent: Monday, June 28, 2010 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unstained Slides Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unstained Slides
We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT. There is no set rule for stability. It all depends on fixation, the nature of the protein target, and storage conditions. I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks. We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal control, you better cut fresh sections. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 6/28/2010 11:54 AM >>> Histoland, How is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
