RE: [Histonet] problem eyes
We use it for fatty samples (we do lots of breast) and yes, it does work well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 04, 2009 3:28 PM To: histonet@lists.utsouthwestern.edu; MargaretPerry; Va Paula Sicurello Subject: Re: [Histonet] problem eyes Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. René J. --- On Wed, 3/4/09, Va Paula Sicurello vapat...@yahoo.com wrote: From: Va Paula Sicurello vapat...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, MargaretPerry margaret.pe...@sdstate.edu, rjbu...@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Perry, Margaret margaret.pe...@sdstate.edu Date: Wednesday, March 4, 2009, 9:14 PM Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). rené J. --- On Wed, 3/4/09, Perry, Margaret margaret.pe...@sdstate.edu wrote: From: Perry, Margaret margaret.pe...@sdstate.edu Subject: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem eyes
We also addes 4-5 drops of Tween 20 in waterbath. Human eye's sections have not any wrinlkes. Maxim Peshkov, Russia, Taganrog. --- On Wed, 3/4/09, Perry, Margaret From: Perry, Margaret margaret.pe...@sdstate.edu Subject: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem eyes
Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Perry, Margaret margaret.pe...@sdstate.edu Date: Wednesday, March 4, 2009, 9:14 PM Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). rené J. --- On Wed, 3/4/09, Perry, Margaret margaret.pe...@sdstate.edu wrote: From: Perry, Margaret margaret.pe...@sdstate.edu Subject: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem eyes
Yes, really with any type of large (and small) sections. The liquid soap will reduce the surface tension of water (more than heat) allowing the sections to expand easily. Try it.. René J. --- On Wed, 3/4/09, Va Paula Sicurello vapat...@yahoo.com wrote: From: Va Paula Sicurello vapat...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, MargaretPerry margaret.pe...@sdstate.edu, rjbu...@yahoo.com Date: Wednesday, March 4, 2009, 4:23 PM Really? Liquid laundry soap? Does this help with all wrinkles in large specimens? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/4/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Perry, Margaret margaret.pe...@sdstate.edu Date: Wednesday, March 4, 2009, 9:14 PM Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). rené J. --- On Wed, 3/4/09, Perry, Margaret margaret.pe...@sdstate.edu wrote: From: Perry, Margaret margaret.pe...@sdstate.edu Subject: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 4, 2009, 4:04 PM Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problem eyes
My suggestion would be to follow the procedure of the old ocular guru from the AFIP, Peter Emanuale. He taught me how the section human and rabbit eyes. It involves using 2 water baths, one of which is at room temp... and you should not use charged/coated slides. Check our his chapter on ocular histotechnology in the 1992 edition of the AFIP Lab Methods manual (it has step by step illustrations) Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_conno...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, March 04, 2009 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem eyes Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem eyes
do a search on the histonet archive i posted a method quite a few years back processed and cut eyes (human and piggy) on a daily basis for almost 10 years go find it - it may help patience, determination, thicker sections, 2 x pre-float out first on dist water and then very dilute alcohol to manually tease out the crinkles, then a gentle float on low ttemp water bath if its too hot and the sections are too thin they will shatter on the warm water plenty of wax support in the block (use a bigger mould when you embed) i am on vacation and saw this post by chance unable to reply in too much detail good luck Annie (visiting in Africa) 2009/3/4 Perry, Margaret margaret.pe...@sdstate.edu Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
