We also addes 4-5 drops of Tween 20 in waterbath. Human eye's sections have not any wrinlkes. Maxim Peshkov, Russia, Taganrog.
--- On Wed, 3/4/09, Perry, Margaret > From: Perry, Margaret <margaret.pe...@sdstate.edu> > Subject: [Histonet] problem eyes > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Date: Wednesday, March 4, 2009, 4:04 PM > > Please help! We have been trying to cut whole eyes > from a pig. They need to be > nice enough for a publication. We are having the > devil of a time because no > matter what we do there are wrinkles. If we turn > the waterbath up to stretch > things out the retina detaches. Do any of you have suggestions? We uses > Surgipath EM400 for embedding paraffin and and > their infiltration medium. Is > this to soft? The lowest we can turn our waterbath is 38 degrees C. > > Margaret Perry HT (ASCP) > IHC Lab Manager Veterinary Science > Animal Disease Research and Diagnostic Lab > South Dakota State University > Box 2175 North Campus Drive > Brookings SD 57007. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
