Re: [spctools-discuss] Multi core analysis
If you're running low-res searches (i.e. fragment_bin_size = 1.0005) then you can probably afford to set this value even higher. Just try starting a search and keeping an eye on memory use. As soon as each batch of spectra is loaded and being searched against the sequence database, that should be approximately the maximum amount of memory that Comet will be using. So you want to set the batch size as big as possible without running out of memory, say shoot for 80% to 90% memory use. The larger the batch size, the more efficient the search is because it reduces some redundant calculations. At some point, the performance gains do level off. Figure 3 of the manuscript "A deeper look into Comet--implementation and features." shows the trade off between batch size, memory use and run times: http://link.springer.com/article/10.1007%2Fs13361-015-1179-x On Wed, Aug 10, 2016 at 1:02 PM, Eslam Nouri wrote: > Hi Jimmy, > > Thanks a lot for the recommendation. I changed the spectra batch size from > 1000 to 5000 and could analyze the same DDA sample with 50% improvement in > the analysis time. I will surely check the 1 spectra batch size to see > if I can obtain shorter analysis time. > > Thanks, Eslam > > On Wed, Aug 10, 2016 at 7:54 AM, Jimmy Eng wrote: > >> How much memory/RAM do you have on your computer? It won't make a huge >> difference but larger spectrum batch sizes are more efficient. So see if >> you have enough free memory to run with that parameter set at say 5000. >> With our 16GB linux computers performing high-res ms/ms searches, I use >> batch sizes of 1. >> >> On Wed, Aug 10, 2016 at 12:27 AM, Eslam Nouri >> wrote: >> >>> Thanks a lot Jimmy. It is a relief to know that I am doing the best that >>> I can do with my current system. >>> >>> I have already removed all the dynamic modifications that I had (no >>> methionine oxidation or phosphorylations were defined). I have also >>> confined the analysis to 1000 spectra batch size. Database search takes a >>> long time, but it identifies a large number of microbiome proteins with >>> high confidence. >>> >>> Eslam >>> >>> On Tue, Aug 9, 2016 at 11:39 PM, Jimmy Eng wrote: >>> Eslam, Setting "num_threads = 0" is the best you can do. There's no free lunch ... a database that's 100 times larger than the human UniProt fasta will take approximately 100 times longer to run, e.g. a 10 minute human search would translate to a 17 hour search if the database were 100 times larger. You can give things up (no variable modifications, no missed cleavages, narrow precursor mass tolerance, etc.) to hopefully speed things up a little bit. Otherwise you just have to throw more horsepower at it. You can get Broadwell-class Xeon processors with up to 24 cores in them. On Tue, Aug 9, 2016 at 11:30 PM, Eslam Nouri wrote: > Hi Eric, > > I am using comet. In my comet file the number of threads is equal to > zero "num_threads = 0". I have attached my system information. Is > there any way to increase the data analysis time by defining a thread > number? > > Thanks, Eslam > > On Tue, Aug 9, 2016 at 11:18 PM, Eric Deutsch < > edeut...@systemsbiology.org> wrote: > >> Which search engine are you using? Comet? Tandem? >> >> >> >> Comet has num_threads: >> >> http://comet-ms.sourceforge.net/parameters/parameters_201601 >> /num_threads.php >> >> >> >> Tandem has threads: >> >> http://www.thegpm.org/TANDEM/api/st.html >> >> >> >> >> >> >> >> *From:* spctools-discuss@googlegroups.com [mailto: >> spctools-discuss@googlegroups.com] *On Behalf Of *Eslam Nouri >> *Sent:* Tuesday, August 9, 2016 11:05 PM >> *To:* spctools-discuss@googlegroups.com >> *Subject:* [spctools-discuss] Multi core analysis >> >> >> >> Hello_ >> >> >> >> I am using TPP for the database search of microbiome proteins. Since >> the fasta file that I have is more than 100 times larger than human >> uniprot >> fasta file, the database search for a single DDA run takes more than 12 >> hours. Is there any way to have faster analysis using multi-cores? >> >> >> >> Thanks, Eslam >> >> Proteomics core facility >> >> University of southern California >> >> -- >> You received this message because you are subscribed to the Google >> Groups "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, >> send an email to spctools-discuss+unsubscr...@googlegroups.com. >> To post to this group, send email to spctools-discuss@googlegroups. >> com. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> >> -- >> You received this message bec
Re: [spctools-discuss] Multi core analysis
Hi Jimmy, Thanks a lot for the recommendation. I changed the spectra batch size from 1000 to 5000 and could analyze the same DDA sample with 50% improvement in the analysis time. I will surely check the 1 spectra batch size to see if I can obtain shorter analysis time. Thanks, Eslam On Wed, Aug 10, 2016 at 7:54 AM, Jimmy Eng wrote: > How much memory/RAM do you have on your computer? It won't make a huge > difference but larger spectrum batch sizes are more efficient. So see if > you have enough free memory to run with that parameter set at say 5000. > With our 16GB linux computers performing high-res ms/ms searches, I use > batch sizes of 1. > > On Wed, Aug 10, 2016 at 12:27 AM, Eslam Nouri > wrote: > >> Thanks a lot Jimmy. It is a relief to know that I am doing the best that >> I can do with my current system. >> >> I have already removed all the dynamic modifications that I had (no >> methionine oxidation or phosphorylations were defined). I have also >> confined the analysis to 1000 spectra batch size. Database search takes a >> long time, but it identifies a large number of microbiome proteins with >> high confidence. >> >> Eslam >> >> On Tue, Aug 9, 2016 at 11:39 PM, Jimmy Eng wrote: >> >>> Eslam, >>> >>> Setting "num_threads = 0" is the best you can do. There's no free lunch >>> ... a database that's 100 times larger than the human UniProt fasta will >>> take approximately 100 times longer to run, e.g. a 10 minute human search >>> would translate to a 17 hour search if the database were 100 times larger. >>> You can give things up (no variable modifications, no missed cleavages, >>> narrow precursor mass tolerance, etc.) to hopefully speed things up a >>> little bit. Otherwise you just have to throw more horsepower at it. You >>> can get Broadwell-class Xeon processors with up to 24 cores in them. >>> >>> On Tue, Aug 9, 2016 at 11:30 PM, Eslam Nouri >>> wrote: >>> Hi Eric, I am using comet. In my comet file the number of threads is equal to zero "num_threads = 0". I have attached my system information. Is there any way to increase the data analysis time by defining a thread number? Thanks, Eslam On Tue, Aug 9, 2016 at 11:18 PM, Eric Deutsch < edeut...@systemsbiology.org> wrote: > Which search engine are you using? Comet? Tandem? > > > > Comet has num_threads: > > http://comet-ms.sourceforge.net/parameters/parameters_201601 > /num_threads.php > > > > Tandem has threads: > > http://www.thegpm.org/TANDEM/api/st.html > > > > > > > > *From:* spctools-discuss@googlegroups.com [mailto: > spctools-discuss@googlegroups.com] *On Behalf Of *Eslam Nouri > *Sent:* Tuesday, August 9, 2016 11:05 PM > *To:* spctools-discuss@googlegroups.com > *Subject:* [spctools-discuss] Multi core analysis > > > > Hello_ > > > > I am using TPP for the database search of microbiome proteins. Since > the fasta file that I have is more than 100 times larger than human > uniprot > fasta file, the database search for a single DDA run takes more than 12 > hours. Is there any way to have faster analysis using multi-cores? > > > > Thanks, Eslam > > Proteomics core facility > > University of southern California > > -- > You received this message because you are subscribed to the Google > Groups "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send > an email to spctools-discuss+unsubscr...@googlegroups.com. > To post to this group, send email to spctools-discuss@googlegroups.com > . > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > > -- > You received this message because you are subscribed to the Google > Groups "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send > an email to spctools-discuss+unsubscr...@googlegroups.com. > To post to this group, send email to spctools-discuss@googlegroups.com > . > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout. >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" gr
Re: [spctools-discuss] Multi core analysis
Hi Eslam, Along the lines of what Jimmy mentioned about "giving things up" specifying fully versus partial tryptic really speeds up the search if that is suitable for your sample. I would recommend talking to your IT department to see if they have any ready solutions for increasing your computing power. They may have resources available that you are not aware of. For example, I talked to the IT Dept. here and they happily set up a 12 core virtual machine that I installed TPP on. They would have done more, but ultimately they wanted to charge me a yearly fee and I decided to go the less expensive route and buy pre-owned "pizza boxes" with dual hex cores, install Ubuntu and TPP myself. These are relatively inexpensive on E-Bay for ~$500 depending on configuration. Here is one example: http://www.ebay.com/itm/HP-Proliant-DL360-G7-Server-2x-X5670-2-93GHz-HexaCore-128GB-4x-450GB-2x-power-/322197730383?hash=item4b047b344f:g:FOAAAOSwtnpXpQMR You may be able to work with such companies that sell these units to have them configured to your specs. So the above would be for just one shared memory system with 12 cores (+12 virtual cores if you config it that way since these processors support hyper theading, which may or may not speed things up. Apparently with hyper threading more isn't always better). Using Ubuntu or other Linux distros, you can set up a cluster of many of these inexpensive boxes that will work in parallel to further increase your processing power. With 5 such boxes, mentioned above, you could have a 60 core cluster for less than $3000 in hardware. Ultimately its your budget and ability to do things yourself that will determine what is best for you. Cheers, Brian Brian Hampton Protein Analysis Lab Center for Vascular and Inflammatory Diseases University of Maryland School of Medicine 655 West Baltimore Street BRB 7-018 Baltimore MD 21201 V: 410-706-8207 On Wed, Aug 10, 2016 at 2:05 AM, Eslam Nouri wrote: > Hello_ > > > > I am using TPP for the database search of microbiome proteins. Since the > fasta file that I have is more than 100 times larger than human uniprot > fasta file, the database search for a single DDA run takes more than 12 > hours. Is there any way to have faster analysis using multi-cores? > > > > Thanks, Eslam > > Proteomics core facility > > University of southern California > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To post to this group, send email to spctools-discuss@googlegroups.com. > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
Re: [spctools-discuss] Multi core analysis
How much memory/RAM do you have on your computer? It won't make a huge difference but larger spectrum batch sizes are more efficient. So see if you have enough free memory to run with that parameter set at say 5000. With our 16GB linux computers performing high-res ms/ms searches, I use batch sizes of 1. On Wed, Aug 10, 2016 at 12:27 AM, Eslam Nouri wrote: > Thanks a lot Jimmy. It is a relief to know that I am doing the best that I > can do with my current system. > > I have already removed all the dynamic modifications that I had (no > methionine oxidation or phosphorylations were defined). I have also > confined the analysis to 1000 spectra batch size. Database search takes a > long time, but it identifies a large number of microbiome proteins with > high confidence. > > Eslam > > On Tue, Aug 9, 2016 at 11:39 PM, Jimmy Eng wrote: > >> Eslam, >> >> Setting "num_threads = 0" is the best you can do. There's no free lunch >> ... a database that's 100 times larger than the human UniProt fasta will >> take approximately 100 times longer to run, e.g. a 10 minute human search >> would translate to a 17 hour search if the database were 100 times larger. >> You can give things up (no variable modifications, no missed cleavages, >> narrow precursor mass tolerance, etc.) to hopefully speed things up a >> little bit. Otherwise you just have to throw more horsepower at it. You >> can get Broadwell-class Xeon processors with up to 24 cores in them. >> >> On Tue, Aug 9, 2016 at 11:30 PM, Eslam Nouri >> wrote: >> >>> Hi Eric, >>> >>> I am using comet. In my comet file the number of threads is equal to >>> zero "num_threads = 0". I have attached my system information. Is there >>> any way to increase the data analysis time by defining a thread number? >>> >>> Thanks, Eslam >>> >>> On Tue, Aug 9, 2016 at 11:18 PM, Eric Deutsch < >>> edeut...@systemsbiology.org> wrote: >>> Which search engine are you using? Comet? Tandem? Comet has num_threads: http://comet-ms.sourceforge.net/parameters/parameters_201601 /num_threads.php Tandem has threads: http://www.thegpm.org/TANDEM/api/st.html *From:* spctools-discuss@googlegroups.com [mailto: spctools-discuss@googlegroups.com] *On Behalf Of *Eslam Nouri *Sent:* Tuesday, August 9, 2016 11:05 PM *To:* spctools-discuss@googlegroups.com *Subject:* [spctools-discuss] Multi core analysis Hello_ I am using TPP for the database search of microbiome proteins. Since the fasta file that I have is more than 100 times larger than human uniprot fasta file, the database search for a single DDA run takes more than 12 hours. Is there any way to have faster analysis using multi-cores? Thanks, Eslam Proteomics core facility University of southern California -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout. >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools-discuss+unsubscr...@googlegroups.com. >>> To post to this group, send email to spctools-discuss@googlegroups.com. >>> Visit this group at https://groups.google.com/group/spctools-discuss. >>> For more options, visit https://groups.google.com/d/optout. >>> >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discuss+unsubscr...@googlegroups.com. >> To post to this group, send email to spctools-discuss@googlegroups.com. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group an
Re: [spctools-discuss] Multi core analysis
Thanks a lot Jimmy. It is a relief to know that I am doing the best that I can do with my current system. I have already removed all the dynamic modifications that I had (no methionine oxidation or phosphorylations were defined). I have also confined the analysis to 1000 spectra batch size. Database search takes a long time, but it identifies a large number of microbiome proteins with high confidence. Eslam On Tue, Aug 9, 2016 at 11:39 PM, Jimmy Eng wrote: > Eslam, > > Setting "num_threads = 0" is the best you can do. There's no free lunch > ... a database that's 100 times larger than the human UniProt fasta will > take approximately 100 times longer to run, e.g. a 10 minute human search > would translate to a 17 hour search if the database were 100 times larger. > You can give things up (no variable modifications, no missed cleavages, > narrow precursor mass tolerance, etc.) to hopefully speed things up a > little bit. Otherwise you just have to throw more horsepower at it. You > can get Broadwell-class Xeon processors with up to 24 cores in them. > > On Tue, Aug 9, 2016 at 11:30 PM, Eslam Nouri wrote: > >> Hi Eric, >> >> I am using comet. In my comet file the number of threads is equal to zero >> "num_threads = 0". I have attached my system information. Is there any >> way to increase the data analysis time by defining a thread number? >> >> Thanks, Eslam >> >> On Tue, Aug 9, 2016 at 11:18 PM, Eric Deutsch < >> edeut...@systemsbiology.org> wrote: >> >>> Which search engine are you using? Comet? Tandem? >>> >>> >>> >>> Comet has num_threads: >>> >>> http://comet-ms.sourceforge.net/parameters/parameters_201601 >>> /num_threads.php >>> >>> >>> >>> Tandem has threads: >>> >>> http://www.thegpm.org/TANDEM/api/st.html >>> >>> >>> >>> >>> >>> >>> >>> *From:* spctools-discuss@googlegroups.com [mailto:spctools-discuss@googl >>> egroups.com] *On Behalf Of *Eslam Nouri >>> *Sent:* Tuesday, August 9, 2016 11:05 PM >>> *To:* spctools-discuss@googlegroups.com >>> *Subject:* [spctools-discuss] Multi core analysis >>> >>> >>> >>> Hello_ >>> >>> >>> >>> I am using TPP for the database search of microbiome proteins. Since the >>> fasta file that I have is more than 100 times larger than human uniprot >>> fasta file, the database search for a single DDA run takes more than 12 >>> hours. Is there any way to have faster analysis using multi-cores? >>> >>> >>> >>> Thanks, Eslam >>> >>> Proteomics core facility >>> >>> University of southern California >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools-discuss+unsubscr...@googlegroups.com. >>> To post to this group, send email to spctools-discuss@googlegroups.com. >>> Visit this group at https://groups.google.com/group/spctools-discuss. >>> For more options, visit https://groups.google.com/d/optout. >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools-discuss+unsubscr...@googlegroups.com. >>> To post to this group, send email to spctools-discuss@googlegroups.com. >>> Visit this group at https://groups.google.com/group/spctools-discuss. >>> For more options, visit https://groups.google.com/d/optout. >>> >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discuss+unsubscr...@googlegroups.com. >> To post to this group, send email to spctools-discuss@googlegroups.com. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To post to this group, send email to spctools-discuss@googlegroups.com. > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
Re: [spctools-discuss] Multi core analysis
Eslam, Setting "num_threads = 0" is the best you can do. There's no free lunch ... a database that's 100 times larger than the human UniProt fasta will take approximately 100 times longer to run, e.g. a 10 minute human search would translate to a 17 hour search if the database were 100 times larger. You can give things up (no variable modifications, no missed cleavages, narrow precursor mass tolerance, etc.) to hopefully speed things up a little bit. Otherwise you just have to throw more horsepower at it. You can get Broadwell-class Xeon processors with up to 24 cores in them. On Tue, Aug 9, 2016 at 11:30 PM, Eslam Nouri wrote: > Hi Eric, > > I am using comet. In my comet file the number of threads is equal to zero > "num_threads > = 0". I have attached my system information. Is there any way to increase > the data analysis time by defining a thread number? > > Thanks, Eslam > > On Tue, Aug 9, 2016 at 11:18 PM, Eric Deutsch > wrote: > >> Which search engine are you using? Comet? Tandem? >> >> >> >> Comet has num_threads: >> >> http://comet-ms.sourceforge.net/parameters/parameters_201601 >> /num_threads.php >> >> >> >> Tandem has threads: >> >> http://www.thegpm.org/TANDEM/api/st.html >> >> >> >> >> >> >> >> *From:* spctools-discuss@googlegroups.com [mailto:spctools-discuss@googl >> egroups.com] *On Behalf Of *Eslam Nouri >> *Sent:* Tuesday, August 9, 2016 11:05 PM >> *To:* spctools-discuss@googlegroups.com >> *Subject:* [spctools-discuss] Multi core analysis >> >> >> >> Hello_ >> >> >> >> I am using TPP for the database search of microbiome proteins. Since the >> fasta file that I have is more than 100 times larger than human uniprot >> fasta file, the database search for a single DDA run takes more than 12 >> hours. Is there any way to have faster analysis using multi-cores? >> >> >> >> Thanks, Eslam >> >> Proteomics core facility >> >> University of southern California >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discuss+unsubscr...@googlegroups.com. >> To post to this group, send email to spctools-discuss@googlegroups.com. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discuss+unsubscr...@googlegroups.com. >> To post to this group, send email to spctools-discuss@googlegroups.com. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To post to this group, send email to spctools-discuss@googlegroups.com. > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
RE: [spctools-discuss] Multi core analysis
Which search engine are you using? Comet? Tandem? Comet has num_threads: http://comet-ms.sourceforge.net/parameters/parameters_201601/num_threads.php Tandem has threads: http://www.thegpm.org/TANDEM/api/st.html *From:* spctools-discuss@googlegroups.com [mailto: spctools-discuss@googlegroups.com] *On Behalf Of *Eslam Nouri *Sent:* Tuesday, August 9, 2016 11:05 PM *To:* spctools-discuss@googlegroups.com *Subject:* [spctools-discuss] Multi core analysis Hello_ I am using TPP for the database search of microbiome proteins. Since the fasta file that I have is more than 100 times larger than human uniprot fasta file, the database search for a single DDA run takes more than 12 hours. Is there any way to have faster analysis using multi-cores? Thanks, Eslam Proteomics core facility University of southern California -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
