[aroma.affymetrix] Re: Error in ACC using custom cdf

2009-02-03 Thread David Rosenberg
Thank you greatly for your help. A couple of additional questions: 1.) With the affxparser writeCdfx functions, it looks as though you are directly writing the binary data of correct endianness rather than inlining/swig-ing the fusion sdk functions. Am I understanding this correctl

[aroma.affymetrix] Re: changing colours in plotRle

2009-02-03 Thread Mark Robinson
On 04/02/2009, at 1:24 PM, Sebastien Gerega wrote: > > Thanks Mark, > that takes care of changing the colours but for some reason the names > argument still isn't influencing anything. For example: > > bpstats = boxplotStats(getChipEffectSet(qam), type="NUSE") > plotBoxplotStats(bpstats, boxfill

[aroma.affymetrix] Re: changing colours in plotRle

2009-02-03 Thread Sebastien Gerega
Thanks Mark, that takes care of changing the colours but for some reason the names argument still isn't influencing anything. For example: bpstats = boxplotStats(getChipEffectSet(qam), type="NUSE") plotBoxplotStats(bpstats, boxfill=c("red","blue","green"), names=c("G1", "G2", "G3")) does not c

[aroma.affymetrix] Re: changing colours in plotRle

2009-02-03 Thread Mark Robinson
Hi Sebastien. My apologies, made a suggestion without running it myself. Try something like: qam <- QualityAssessmentModel(plm) bpstats <- boxplotStats( getChipEffectSet(qam), type="RLE" ) # put in your specific arguments below ... plotBoxplotStats(bpstats, boxwex=.2, boxfill=c("red","blue"

[aroma.affymetrix] Re: changing colours in plotRle

2009-02-03 Thread Sebastien Gerega
Hi Mark, thanks for your reply. I had actually tried several commands along those lines but always get the following: Error in boxplot.stats(theta - medianLE, ...) : unused argument(s) (boxwex = 0.4) or: Error in boxplot.stats(theta - medianLE, ...) : unused argument(s) (boxfill = "blue") T

[aroma.affymetrix] Re: changing colours in plotRle

2009-02-03 Thread Mark Robinson
Hi Sebastien. Note that plotRle() eventually makes a call to 'bxp' (in the graphics package that is loaded by default) and any/all arguments are passed on. Have a look at ?bxp for what you can specify. For example: [...] qamTr <- QualityAssessmentModel(plmTr) plotRle(qamTr, boxwex=[something]

[aroma.affymetrix] changing colours in plotRle

2009-02-03 Thread Sebastien Gerega
Hi, is there a way to change the colour of each individual bargraph when calling plotRle? I would like to make the colours correspond to test groups. thanks, Sebastien --~--~-~--~~~---~--~~ When reporting problems on aroma.affymetrix, make sure 1) to run the late

[aroma.affymetrix] Re: LOH / UPD analysis

2009-02-03 Thread Henrik Bengtsson
Hi, there are no methods of such analysis available in aroma.affymetrix, and there is no time line for implementing that. It is of course on our wishlist, though. Note also that "LOH analysis" is rather unspecific; there are many different ways to infer LOH, of which some depend on what kind of

[aroma.affymetrix] Re: Chromosome explorer - zooms 64 + 128.

2009-02-03 Thread Henrik Bengtsson
Hi, especially those zoom levels generate very large PNGs. They might be too large for your PNG device driver. Depending on your system, there are few different PNG drivers available to R. You can override the one that aroma.affymetrix uses, but overriding the findPngDevice() function. Search

[aroma.affymetrix] Re: Error: cannot allocate vector of size 692.8 Mb

2009-02-03 Thread Henrik Bengtsson
Some comments: 1. The problem occurs in the internal method getUnitGroupCellMap(). This is only called once per chip type, regardless if you restart R or not. The result is memoized (=cached to file) and reused in the future. Thus, if you can get it to work once, you won't have the problem anym

[aroma.affymetrix] Re: Error in ACC using custom cdf

2009-02-03 Thread David Rosenberg
Sorry, changed network points and reposted by accident. Thanks for your help. This should be enough to get me thinking for a bit. On Feb 3, 2009, at 3:23 PM, Henrik Bengtsson wrote: > > Ok, sorry I didn't answer you question explicitly. No, they do not > have to contain ChrX and ChrY data.

[aroma.affymetrix] Re: Error in ACC using custom cdf

2009-02-03 Thread Henrik Bengtsson
Ok, sorry I didn't answer you question explicitly. No, they do not have to contain ChrX and ChrY data. However, but independent of your question, I would recommend that you import all annotation data available, since that might become of interest in future usage. Currently part of the code is h

[aroma.affymetrix] Re: Crash in using RmaCnPlm with combineAlleles = FALSE

2009-02-03 Thread Henrik Bengtsson
Hi, the factor of two has to do with the number of parameters estimated/stored, i.e. storing theta, vs (thetaA, thetaB). I cannot reproduce this, but I one guess is that you have tried both combineAlleles=TRUE and combineAlleles=FALSE and somehow there exist cached indices that are reused from o

[aroma.affymetrix] Re: Error in ACC using custom cdf

2009-02-03 Thread David Rosenberg
Do they ufl/ugp/acc files need to be build with X and Y chromosomes included? If so, this won't be too hard to fix. On Jan 30, 9:50 pm, David Rosenberg wrote: > As I think about this further, it occurs to me that there are other   > potential problems with this chip/cdf.  I was looking at the

[aroma.affymetrix] Re: Error in ACC using custom cdf

2009-02-03 Thread Henrik Bengtsson
Hi, I'm swamped and still haven't had a chance to create a real fix, but a quick fix is to override the arguments that defaults to "-XY", but replacing them with an integer/index vector specifying the *units* on the autosomal chromosomes, that is, the ones that are are expected to be copy neutral

[aroma.affymetrix] Re: Installing on R 2.8.1

2009-02-03 Thread Henrik Bengtsson
Hi Michal, it shouldn't be tedious at all - did you follow the instructions? source("http://www.braju.com/R/hbLite.R";) hbInstall("aroma.affymetrix") URL: http://groups.google.com/group/aroma-affymetrix/web/download-install That should install R.huge v0.1.6 as well, but if it for some reason d

[aroma.affymetrix] Installing on R 2.8.1

2009-02-03 Thread michal.blazejczyk
Hello, I am trying to install aroma.affymetrix on R 2.8.1 (and the Bioconductor version that goes with it). After a long and tedious process of downloading missing packages, I'm stuck at the following: Error: package 'R.huge' 0.1.5 was found, but >= 0.1.6 is required by 'aroma.affymetrix' Is i

[aroma.affymetrix] Re: Error in ACC using custom cdf

2009-02-03 Thread David Rosenberg
Do they ufl/ugp/acc files need to be build with X and Y chromosomes included? If so, this won't be too hard to fix. On Jan 30, 9:50 pm, David Rosenberg wrote: > As I think about this further, it occurs to me that there are other   > potential problems with this chip/cdf.  I was looking at the

[aroma.affymetrix] LOH / UPD analysis

2009-02-03 Thread mbaudis
Dear all, AFAIK there is no method for LOH / UPD analysis implemented in a.a (yet?)? Is there a timeline for that? Does anybody have an established workflow for that, e.g. low level processing in a.a, and then using a separate solution? Any comments are appreciated! Thanks, Michael. IMB, Univ