Dear all,
Please could you bring this advert to the attention of any potential
candidates?
A 4-year Ph.D. studentship in protein crystallography is available in
the Laboratory of Crystal and Structural Chemistry at Utrecht University.
The successful applicant will work on fascinating
Yes, this script should work. You can also use OUTPUT CNS.
But the GUI is not up-to-date with the program. This is fixed now. You
can pick up fixes via the Problems Page
http://www.ccp4.ac.uk/problems.php#6.0.2-export-hl
or it will be in the next release.
Tip for newbies: you can always get
Hi all,
I'm looking for some advices on some general hints on how to carry
out a MAD/SAD data collection.
We had SeMet crystals that diffracted to ca. 3.5 Angs, with anomalous
signal only at ca. 5-5.5 Angs, with diffraction decaying on brilliant
beamlines (ID29 or ID23 at the ESRF) in a
Hi Sebastiano, think of it this way: it's not your crystals that decay
within 300 degrees, it's you who let them decay that quickly, by not
attenuating the beam sufficiently.
But now that you know approximately how long they survive (which won't
change just because the crystal is bigger, I
Hi folks,
I've been an AKTA user for probably close to ten years, and in this time
I've also used BioRad and several other systems. I'd like to make a couple
of comments since everyone seems to be enjoying this lively discussion :)
0. Choose the device for what *you* need. Some applications are
Hi,
When I tried to install CNS in my linux machine (Fedora core 4.0), I got the
error message when compiling
compiling: machine_f.f
In file machine_f.f:393
ERR=.TRUE.
1
In file machine_f.f:297
2
Error: Label at (1) is not in the same block as the GOTO statement at
Hi, Axel,
Thanks for the hint.It works now with g77 compiler.
Jenny
On 2/15/07, Axel Brunger [EMAIL PROTECTED] wrote:
Which linux machine version of CNS are you using? 32 or 64 bit? Make
sure you use Gfortran or Ifort compilers.
Axel Brunger
Jenny wrote:
Hi,
When I tried to install
Hi,
Long-time reader, first-time writer to ccp4bb.
I'm having a problem with a 3.0 angstrom dataset (2.8 if I push it).
These crystals were thin but very long needles and I could see
diffraction only at the synchrotron. Diffraction spots were very
very small but well defined. High
I prefer HPLC too, and am currently using a Waters LC for all my protein
purifications. I have also used a Dionex Bio-LC in the past, which does
have non-corroding PEEK pump heads. It was a great system.
Peter
On 2/14/07 8:18 PM, Daniel Anderson [EMAIL PROTECTED] wrote:
It still seems to
Pedant's corner:
The systems are actually called ÄKTA (loosely the real thing in
Swedish) and not AKTA (watch out!, beware!), although given the
feelings expressed about the price, the latter may not be such a
misnomer ;-)
Phew, I got there before Gerard!
Derek
--
Derek Logan
I guess I would go back to the data.
Could there be twinning?
Is the a pseudo translation vector which means there are systematically
weak zones of data? ( hklview might show something strange)
Rfree seems rather close to R to me for this resolution - maybe it
should go up a bit?
How many
Dear all,
Thanks for the almost 100 replies I have received. I appreciate every one of
them very much. Since some asked for a summary, here it is, albeit with more
questions. :-)
Assuming you buy your own computer and put your equipment in the cold room,
(1) AKTA (or whatever it is spelled)
Peter,
The R/Rfree values seem reasonable given the resolution of the data and
the completeness of the model. If you are unable to improve the model I
would suggest getting an experimentally phased solution MAD or MIRAS.
If your starting MR model is too incomplete you may not be able to get
past
Hi Peter,
After you have finished checking the data again for twinning, etc.
as suggested by Eleanor,
you might try the following:
1) Refine using the simulated annealing refinement protocols in CNS and
then check R-values and map quality. If you try that, you can also test
a few different
Although I have not yet tried to compile coot or CCP4, I have found that the
GNU provided packages (gcc, g77) do not make life convenient (how is that for a
euphamism for 'banging your head against the wall)?
Things worked better with gfortran than g77 and again better with the Intel
I have used the Intel compilers, and yes, they work pretty well. However,
they do not solve this particular problem. I have one problem with them; if
you read the fine print on the academic license, you find that you are not
supposed to use them for things other than your own research unless you
The Intel Fortran compiler produces code that is indeed significantly faster
than code generated with g77 or gfortran, but recent g++ (and most likely gcc)
versions consistently produce code that is at least as faster or faster than
code generated with Intel's C++ compiler.
Bottom line: it
Its much better to refer to your machinery by its first name they work
better that way. My AKTA machines always had Teutonic names: Helga,
Brunhilde, Gertrude, just to name the last three.
Artem
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Derek
Logan
As far as i understand the intel small print,
you are allowed to distribute binaries compiled with intel compilers,
along with s a few intel libraries that are required by the libraries
you compile.
But not sell them i think.
Dan
On 16 Feb 2007, at 01:34, Lynn Ten Eyck wrote:
I have used
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