I presume you tried P32 21 also? Pointless cannot distinguish the
difference..
Refinement at 3A is tricky - you can overfit quite easily and maybe
Rfree is correctly flagging that there are still errors.
Can you see features which need rebuilding?
Eleanor
Emmanuel Prata wrote:
Dear all,
I
I have used many methods to do this over the years, but now I would
never consider using anything but the COOT interface.
The NCS operators that COOT uses are based on the SSM (secondary
structure matching) program written by Eugene Krissenel.
You read in your coordinates into coot and it re
High symmetry for me means F432 .. no particular problem in H32
And of course the more molecules to find in the asymmetric unit the more
difficult it is..
But the general rule is
Rotation function harder the higher the symmetry - more likely to have
overlap between crystal symmetry and NCS
Tr
Announcement of a Symposium in honour of Professor Dame Louise Johnson,
Oxford University, Sep 2nd to 5th 2007
Themes of the symposium will include protein structure, signal
transduction, protein phosphorylation, the cell cycle, enzymatic
mechanisms, synchrotron radiation, electron microscopy, dru
Hi David,
Do I have to register for the meeting as an invited speaker?
Liz
Dr. Liz Duke
Principal Beamline Scientist
Diamond Light Source
Rutherford Appleton Laboratory
Chilton
OX11 0QX
Tel 01235 778057
FAX 01235 778448
Mobile: 07920138148
-Original Message-
From: CCP4 bulletin boar
Dear All:
We have only a few more places left for the two day workshop on
drug target crystallography and structure-based drug design
to take place on June 28-29, 2007 in La Jolla, California.
The workshop will be conducted jointly by Dr. Ruben
Abagyan (Professor of Molecular Biology at The Scri
The Max Planck Institute for Medical Research has opening for an Independent Junior Research Group (Selbstständige Nachwuchsgruppe der Max-Planck-Gesellschaft). This offer includes, in addition to the group leader position, funding for a research group.
While there is no explicit restriction on
A post-doctoral position is available to study the structure and
function of large catalytic RNA molecules. The work is a combination of
crystallographic, biochemical, and biophysical work and is focused
primarily on understanding the structure and mechanism of RNase P.
Strong previous experien
I have a partially cleaved RNA, so I would like to try refining with
two different dinucleotide segments embedded in the model, one of
which has a broken backbone and 2',3'-cyclic phosphate, and the other
with a standard phosphodiester backbone. This should be analogous to
having two confor
Hi all
we r planning to upgrade hardware in the lab. just curious to know weather
anybody has tested a dual core xeon vs dual core opteron for standard
crystallographic applications. Also wanted to know weather programs compiled
on a xeon will have problems executing on an opteron.
regards
vin
Hi,
I just found out that my magic file contains a large section with respect to
crystallographic file formats. I was pretty impressed. According to the
header it is attributed to Joe Krahn. Full ACK to him!
Tim
--
Tim Grune
Australian Synchrotron
800 Blackburn Road
Clayton, VIC 3168
Austral
The 'magic' file is the database the unix command 'file' uses to determine
different fileformats. It is plain text and jsut describes the first couple
of bytes of each file which are supposed to be enough to determine what type
of file it is (e.g., a PNG-image, or an MTZ-file, or a CNS-map).
;-
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