Hi Jerry,
have you tried tinkering with the pH?
I have had similar situation, where two normally soluble proteins
precipitated upon mixing at pH 7.5, my "stock" buffer. Altering the pH
showed that the complex that was only soluble at pHs lower than 6.
Looking at your pIs, it strikes me that in b
I just noticed there is a space between s and o in .so:
Robert Grant wrote:
Gtk-WARNING **: Unable to locate loadable module in module_path:
"libbluecurve.s o"
Probably a mistake in pasting the error message,
but if not it could be significant.
Ed
Forgot to include that it is a Red Hat EL4 OS.
-- Forwarded message --
From: Robert Grant <[EMAIL PROTECTED]>
Date: Dec 12, 2007 3:42 PM
Subject: upgrading ccp4 broke my Coot stereo
To: ccp4bb@jiscmail.ac.uk
I recently upgraded from ccp4 version 5.99.5 to 6.02.
In doing so I have
Dear Jerry,
One way I can think of would be to try the magical polymer NV-10 sold by
Novexin (UK). It does actually work very well for proteins with low
solubility. You'd add a few mg/ml of polymer to each protein solution, mix
the two and see what happens. This polymer has already saved severa
Hi,
I've recently made a ligand using the Sketcher module in CCP4i and made the cif
dictionary. I can run Refmac5 in "review restraints" mode with the dictionary
in my working directory and it seems to work fine.
However, now I am trying to refine my ligand in the protein structure and I get
t
What is the operating system? These things are system-dependent...
On Wed, 12 Dec 2007 15:42:39 -0500
Robert Grant <[EMAIL PROTECTED]> wrote:
It would appear that an environmental variable involving a library
path is missing or wrong but I have not been able to figure out what
it
I recently upgraded from ccp4 version 5.99.5 to 6.02.
In doing so I have lost the ability to run Coot with hardware stereo.
When I fire up Coot it reports:
Gtk-WARNING **: Unable to locate loadable module in module_path:
"libbluecurve.s o"
When I try to switch to hardware stereo it says:
CATAST
Dear All,
Recently I posted a question about protein induced protein precipitation.
Firstly I'd like to thank many folks for their good ideas.
Later on I did a titration experiment with one protein concentration
fixed at 0.4mg/ml(about 10uM). Now it is clear that these two
Simon,
We routinely obtain structures from protein solutions with a big pellet of
ligand in the bottom of the tube. For co-crystallizations we add 1mM
compound to a 0.3mM solution of the protein and incubate overnight. Many of
the compounds are only soluble to 50micromolar, so we get a lot of
pre
Dear Simon,
DMSO concentrations lower than 5% usually do not alter crystallizability
of a protein. In case you want to avoid this solvent may I suggest you
trying out two methods that worked for me.
1. If you grow crystals in PEGs or similar molecules you might try to
solubilize the compound in a
One method that worked for me was to dissolve my ligand in 100% DMSO, as
suggested in the previous response, then add a 3 molar excess of ligand
to protein so that the final concentration of DMSO in the protein-ligand
solution was no greater than 10% - of course the maximum concentration
of DMSO th
Directed Evolution Approaches in Structural Biology
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