Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-19 Thread Juan Sanchez Weatherby
Dear Cedric, Another possible method for RT data collection is the new humidifier device HC1b developed at the EMBL that is now available for use at the ESRF (see links below). It works in a very similar way to the FMS but a little easier to use and can be used at the ESRF with very little

[ccp4bb] ESCET

2009-01-19 Thread Thomas R. Schneider
Hi, for obtaining ESCET, please contact me directly at: thomas.schnei...@embl-hamburg.de Thanks Thomas --- Dr. Thomas R. Schneider Project Coordinator e...@petra3 EMBL c/o DESY Notkestr. 85

Re: [ccp4bb] Create a dual boot windows-linux machine

2009-01-19 Thread José Trincão
Dear Xie, you should seriously consider what Johan Turkenburg suggested: create a 20~30Gb ext3 patition that you mount as / and the rest of the disk as ext3 mounted as /home. That way, if you have to reinstall linux, all your files will be safe. You would end up with the following

Re: [ccp4bb] is the program ESCET still available?

2009-01-19 Thread Tim Gruene
No, I am afraid I have to apologize, I confused the download address for escet with the one for hkl2map. escet cannot be received through this address. Tim -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Mon, 19 Jan 2009, Boaz

Re: [ccp4bb] Create a dual boot windows-linux machine

2009-01-19 Thread Roger Dodd
Dear all, Just to add, linux now has perfectly good read-write stable drivers for NTFS (the native WinXP filesystem). This means you no longer need to create a separate FAT32 partition for sharing data between the two OSes. Cheers Roger On 19/1/09 11:25, José Trincão wrote: Dear Xie, you

[ccp4bb] Molecular replacement using a partial molecular replacement solution

2009-01-19 Thread Mo Wong
Hello, My problem: I have poorly phased 3.5A data which suggests 6 molecules per ASU, and using MolRep with the experimental phases (search for model in the map) I have good solutions 3 of them. There is a lot of empty electron density which needs to be filled with more copies of the molecule. I

Re: [ccp4bb] Molecular replacement using a partial molecular replacement solution

2009-01-19 Thread Klaus Piontek
Wong, did you look for pseudo-symmetry? For example for pseudo-translation, which you can deduce from a native Patterson. Good luck, Klaus Mo Wong wrote: Hello, My problem: I have poorly phased 3.5A data which suggests 6 molecules per ASU, and using MolRep with the experimental phases

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

2009-01-19 Thread Jeff Christensen
We've been using the Mitegen MicroRT product for a while and it works well, but I'm anxious to try Filip Van Petegem's paratone oil method. I've used paratone as a cryo before, and spent a lot of time trying to wick away the liquid around the crystal - in the case of room-temp mounting the

[ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-19 Thread Torres-Larios Alfredo
Dear all, I know this sounds weird (and besides, it's a non CCP4 question), but does anyone know any reports or has worked with a recombinant protein that is apparently post translationally modified by E. coli? We have an enzyme with two populations that have been sequenced at the

[ccp4bb] Raxis II - free to good home!

2009-01-19 Thread Pamela Focia
We have an Raxis II system we took down a few months ago, plus an almost full, duplicate set of its major parts that we salvaged from a neighboring University last year. If you can use any or all of it, I'd rather arrange to give it away to you than the metal scavengers. We only have a few

Re: [ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-19 Thread Artem Evdokimov
Hi, Yes, this does happen. Spontaneous α-N-6-Phosphogluconoylation of a His Tag inEscherichia coli:The Cause of Extra Mass of 258 or 178 Da in Fusion Proteins http://www.sciencedirect.com/science?_ob=ArticleURL_udi=B6W9V-45N4K22-R_us

[ccp4bb] plate screening in X-ray beam

2009-01-19 Thread Jean-Luc Ferrer
Title: Lutilisation pour la cristallographie des protines des source Dear all, FIP-BM30A team, at ESRF, has developped a robotized (so called "G-Rob") system to manipulate crystallization plates and microchips. The plates/chips are directly exposed to the X-ray beam, enabling the user