Hi everybody,
Thanks for the information,
Even i was discussing with one my uncle who is in fashion industry,about
these intelligent designs (offcourse the pbd structures).
May be folks..in future you have to wear rna polymerase or hiv protease or
1aka or.
imprinted on holiday shirts..is
You dont say at which point in the refinement cycle you are..
The refinement algorithms are meant to reduce these parameters, and if
they diverge wildly there is probably something seriously wrong either
with the software or the model..
eg - different cell dimensions or space group for the
If you choose one of the structures we solved for your T-shirt
(admittedly not as sexy as rna polymerase or hiv protease or 1aka),
don't worry about acknowledging us.
What's more, if we ever meet you wearing the T-shirt at a conference
or somesuch, you'll be guaranteed a free drink!
Mark
More details? Are there any particular data problems ?
Is twinning a possibility - look at new truncate plots..
Eleanor
Rana Refaey wrote:
Hi all
I have two datasets of resolutions 1.6 and 1.65 Å both of the same molecule,
the problem that i am facing is the refinement.
The R factors are
I have purified my protein granulocyte colony stimulating factor using
chromatography steps. my protein is relatively pure when analysed by
reducing and non-reducing SDS PAGE method. however non-reducing page
shows DIMER presence. i have about 250 ml pure protein sample and do not
want to
Hi all,
let me see your pictures. I'll prepare a CCP4BB
Screensaver for Win(we'll see) Mac (guaranteed).
Each individual may send two of his/her best pictures related to
crystallography, you must have either a) the permission to distribute
this picture or b) be the owner of it (keep in mind if
Jürgen
In the case you have an overwhelming amount of images, why not instead
just setup an automatically generated RSS feed (on a server that you
or maybe the ccp4 project or wiki will host) that contains them?
There is an RSS screensaver already built into leopard, I'm sure there
is
*Postdoctoral fellowship at the High Throughput Crystallization
laboratory of the EMBL Grenoble Outstation*
*EMBL site:* EMBL Grenoble
*Commencing date:* As soon as possible
*Job description:* The High Throughput Crystallization laboratory at the
EMBL Grenoble Outstation is offering a
I think this was posted earlier, but the geometry constraints for the
cartoon mode is a little tight for a residue of my protein. What's the
setting to relax them so it'll draw a continuous tube instead of
leaving it blank?
Thanks
FR
-
Francis
Dear all,
I'd like to express a single chain variable fragment (scFv) for
crystallization purposes, but cannot decide whether I should do it in
insect cells (let the antibody be secreted into culture medium) or
bacteria (let the antibody be secreted into periplasmic space).
If someone could
Hi Lisa,
in addition to what Joern suggested, there are two other options:
1) You can run phenix.metal_coordination that will create additional
restraints for Mg atoms:
phenix.metal_coordination model.pdb
2) Not the best ways of doing this, but just to mention so you have a
complete list
Both will probably work.
Expression in insect cells is most likely to work well, but will of course
be more expensive and time consuming. There's also some slight possibility
of getting unwanted glycosylation - which is not necessarily a problem.
I've had good success expressing VHH fragments
Why don't you add some reducing agent like DTT or BME to make everything as
monomer?
Anthony
On Tue, 17 Feb 2009 10:47:28 +, Meg wrote
I have purified my protein granulocyte colony stimulating factor using
chromatography steps. my protein is relatively pure when analysed by
reducing
Thanks a lot for all your suggestions.
But how much DTT or BME shall i add. Also i fear that these will interfere
in the formulation and how shall i get rid of them then. As for GPC my
protein is 19KDa and diemr will be approx 38 KDa and it is difficult to
separate them without losing the protein
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