Hello Deepak,
What pH are your crystals at? Also, you need to check whether
your atomic arrangement has reasonable geometry for hydrogen bonding
in addition to the interatomic distances.
ho
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Date:Mon, 29 Mar 2010 12:11:59 +0800
From:Deepak Oswal
Subj
If it is a _true_ 4.5 A resolution map, you should be able to build
alpha-helices and beta strands (can buccaneer do that automatically in at
4.5A?) and then use these secondary structure elements to find your known
structures.
Foldhunter from EMAN should be able to build helices and strands au
Dear All,
I have a crystal (not EM) density map of a very large complex at 4.5A
resolution. I have pdb files for homologs of a few of the subunits of this
huge complex. I would like to fit these homologs into the density. I have
tried without success so far programs that handle phased molecular
re
The Laboratory of Molecular Biology (LMB) in Cambridge, UK wishes to
recruit an Investigator Scientist to support a range of collaborative
projects involving biophysical methods for studying macromolecules and
their interactions. The individual will also conduct workshops and
informal tr
Hi Folks,
I have seen this when you take data from XSCALE to merge in Scala -
the former puts I's on the scale I=F^2 making for very large numbers.
I typically "undo" this scaling factor using pointless -c and the
"multiply" keyword. Multiply by 1/the value reported in XSCALE output.
Pointless is
Thank you very much to everyone who replied! Below is a list of the labs and
facilities. I received quotes ranging from 200-330 EURO, and 150-215 GPB for a
5 amino acid read. Better try your own luck with specific quotes..
Tim
Dr Jeff N Keen
Leeds University - Proteomics facility
http://www.f
Dear Crystallographers...
We have an EU funded studentship to study α-Helix Mimetics as Inhibitors of
Protein-Protein Interactions Involved in Cancer Development and Progression.
Please forward to anybody who may be interested.
The purpose of this project is to develop a RULE-BASED APPROACH for
Not knowing more details about the enzyme, etc, makes this a little
difficult. I would think you might need a more polarizing group nearby
to shift the pKa. The first thing that jumps to mind for me is that the
water molecule itself is what is activated in this case, making it
nucleophilic. Ther
Just some ideas. NCS usually good with 2.7A data, TLS only to be used
when model is complete..
You say Matthews suggest 11 molecules, but you only find 6 - doesnt
this mean you have a very high solvent content?
I would check the MR carefully.
How are the 6 molecules related - are there dimer
