[ccp4bb] Postdoc position available

SUMMARY DESCRIPTION: Structural biology of virus-host interactions The Kvansakul Laboratory is seeking a postdoctoral fellow to investigate the structural basis of inhibition of programmed cell death during viral infection (e.g. Kvansakul et al 2007 Mol. Cell 25: 933 ­ 942, Kvansakul et al Cell

Re: [ccp4bb] small lysozyme crystals?

Hi James, Not as many responses on lysozyme crystallization as I would have expected. Or may be people thought you were joking (were you?). Anyhow, higher concentrations of lyso gives smaller crystals but if you let them grow on their own, you may get few huge ones instead. What worked for me w

[ccp4bb] Third issue of Computation Crystallography Newsletter - Volume 2, Number 2

Dear Colleagues, I am pleased to announce the publication of the third issue of the Computational Crystallography Newsletter: http://www.phenix-online.org/newsletter/ A listing of the articles and short communications is given below. Please note that the newsletter accepts articles of a g

Re: [ccp4bb] OSX Lion

On Jul 27, 2011, at 6:45 AM, Bosch, Juergen wrote: > But Bill has updated his webpage

Re: [ccp4bb] Fab:antigen complex crystallization!!!

Ed (and Ivan) Peter Sun and colleagues published two papers where they show that crystallization conditions for protein-protein complexes are strongly biased towards PEG-based rather than high-salt or organic-solvent-based conditions. This includes antibody-antigen complexes. http://www.ncbi.nlm.

[ccp4bb] Position available: X-ray FEL Crystallography

http://www.desy.de/v2/docs/1310651114-e.pdf DESY, Hamburg location, is seeking a Senior Scientist or Postdoc (m/f) to conduct research in the area of X-ray FEL Crystallography. DESY is one of the world's leading centres for the investigation of the structure of matter. DESY develops,runs and uses

Re: [ccp4bb] research paper

The article is available for purchase for $40. Journals cannot survive without funding which can come from many sources - subscriptions, author payment to make the article open-access, etc. But asking someone to provide a 'free' copy without Acta's permission is tantamount to theft.

Re: [ccp4bb] research paper

On Thu, 2011-07-28 at 14:35 +, Jung-Hoon Lee wrote: > Acta Cryst D63 (2007), 550-554. I can't believe Cornell has no access to Acta D. -- "Hurry up before we all come back to our senses!" Julian, King of Lemurs

[ccp4bb] research paper

Dear all, I would appreciate it if anyone could provide me with a copy of this research paper. Thank you very much. A. D'Arcy, F. Villarda, M.Marsh An automated microseed matrix-screening method for protein crystallizationActa Cryst D63 (2007), 550-554. John

Re: [ccp4bb] Fab:antigen complex crystallization!!!

On Thu, 2011-07-28 at 05:07 +0100, Sean Seaver wrote: > Spoiler - Fabs like ammonium sulfate. Not really - in my hands the ammonium sulfate was one hit out of 7. While Ivan's question is about Fab complexes with protein antigen, I think it brings up a more general question of protein class-depend

Re: [ccp4bb] Fab:antigen complex crystallization!!!

Hi Ivan, Here is another example of a method to crystallize antibody/antigene complexes. It uses a limited proteolysis step to generate crystals of poor quality, which are then used as seeds for an MMS screening... http://www.ncbi.nlm.nih.gov/pubmed/21536542 Good luck, Alex 2011/7/28 xaravich

Re: [ccp4bb] Fab:antigen complex crystallization!!!

Hi Ivan Did you use microseeding with *random *solutions? If not see the following paper by Obmolova and Co about exactly this, microseeding with Fab complexes, http://journals.iucr.org/d/issues/2010/08/00/bw5361/bw5361.pdf For more subtle variations in using microseeding with complexes see http

Re: [ccp4bb] ARP/wARP install on 6.2.0 & RHEL 6?

My two cents: I am installing arp/warp on my Mac since ages and it works ;-) The only thing you need to make sure is that you grab ownership of the /usr/local directory to you as a user, if you installed ccp4 from dmg. If you do this by eg sudo chown -R me.mygroup $CCP4 Then install.sh should

[ccp4bb] PostDoctoral Fellow in Synchrotron Crystallography

A postdoctoral position in synchrotron crystallography is available at EMBL Hamburg in the group of Thomas Schneider. If you would like to join an international team of nice and motivated people to work on interesting projects during the commissioning phase of our new beamlines on PETRA III, plea

Re: [ccp4bb] ARP/wARP install on 6.2.0 & RHEL 6?

Hi, I recently found the problem that after finishing the installation of CCP4-6.2.0 on RHEL5, if the installation destination is not the default path (/usr/local/), the ccp4.setup for csh wouldn't take the customized TCLTK path correctly. No matter if I used sh or csh to install the CCP4 package.

Re: [ccp4bb] Fab:antigen complex crystallization!!!

Hi Ivan, you might also want to find out what buffers your particular system likes. Jancarik et al. Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins. Acta Crystallogr D Biol Crystallogr (2004) vol. 60 (Pt 9) pp

Re: [ccp4bb] ARP/wARP install on 6.2.0 & RHEL 6?

Dear all, I had no problem with Arp/wARP, but I didn't reinstall. Assume ccp4 sits in /installation/directory/ccp4 and arp in /installation/directory/arp_warp_7.1. The new ccp4 doesn't recognize the existing arp because the task interface is not installed. I run ./install.sh in /installati

Re: [ccp4bb] ARP/wARP install on 6.2.0 & RHEL 6?

Hello, My reply is in the text below: On Jul 28, 2011, at 6:00 AM, ccp4 wrote: > A plea from West Australia too. > I was sitting with someone yesterday who was trying to install it on a Mac > , and finding it a nightmare. We're working on improving this. I believe the main issue is that CCP4

Re: [ccp4bb] ARP/wARP install on 6.2.0 & RHEL 6?

Hello, I'm one of the ARP/wARP developers. Sorry for the slow reply - this is down to time differences. I'll install RHEL6 and try the ARP/wARP install and then report back. This may take a day or so though. My suspicion, based on previous bug reports, is that this problem is related to the T