Some hints:
Richter et al. De novo enzyme design using Rosetta3. PLoS ONE (2011) vol. 6 (5)
pp. e19230
Fleishman et al. Computational design of proteins targeting the conserved stem
region of influenza hemagglutinin. Science (2011) vol. 332 (6031) pp. 816-21
Röthlisberger et al. Kemp eliminati
A 3-year PhD student position in protein biochemistry/structural
biology is available at the Institute of Biochemistry, Biocenter of
the Julius-Maximilians-University, Wuerzburg/Germany.
The successful applicant will work on the Survival Motor Neuron (SMN)
complex, a key player during the p
Dear all,
My mtz file has no columns for M/ISYM and BATCH. It was generated by xdsconv
from XDS output. Would someone has any ideas how to get these columns in mtz
file?
Many Thanks,
G
If you want to get an unmerged XDS file into Scala for any reason, then you
should use Pointless to do the conversion
Phil
On 3 Aug 2011, at 13:15, G Y wrote:
> Dear all,
>
> My mtz file has no columns for M/ISYM and BATCH. It was generated by xdsconv
> from XDS output. Would someone has any
Dear colleagues,
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9th International NCCR Symposium on New Trends in Structural Biology 1-2
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Dear all,
I am trying to deposit a pdb containing TLS groups. PDB instructions states:
"As we have mentioned on the start page for autodep, entries refined using
REFMAC with TLS parameters must now contain the full B-factor in ATOM/HETATM
records, with the TLS contribution coded into ANISOU recor
Postdoctoral Fellow
Protein Crystallography at SLS
Your tasks
As part of the development of next generation micro-crystallography at the
undulator beamline X06SA, you will be involved in micro-focussing optics and
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Software Engineer
Protein Crystallography Beamlines at SLS
Your tasks
Within a small international software development team you are expected to
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Hi Catarina ,
For your final refmac refinement job, you need to include
the keyword
TLSO ADDU
to have the TLS contribution added to the residual B-factors.
This keyword can also be set from the recent version of the ccp4i
GUI by checking the checkbox for "Add TLS contribution to XYZOUT"
in the TLS
Just wondering: wouldn't running tlsanal on the final refmac output as Catarina
has it now put the true aniso record straight?
Cheers,
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone: 972
Hi Boas,
Yes, tlsanal would work but only if the structure was
refined with the keyword
TLSD waters exclude
either by explicitly including the keyword or
by using a version that of refmac that defaults to
TLSD waters exclude. I believe that recent
versions of refmac 5.6 default to tlsd water
Dear all,
Thanks for the suggestions. I'll give it a try and see how it goes.
Cheers,
Catarina Silva
On Wed, Aug 3, 2011 at 9:59 PM, Miller, Mitchell D. <
mmil...@slac.stanford.edu> wrote:
> Hi Boas,
> Yes, tlsanal would work but only if the structure was
> refined with the keyword
> TLSD wat
Dear all,
I have two unrelated questions. Any suggestion on any of them will be greatly
appreciated.
First, we have two proteins that bind each other without a doubt. But since we
have very limited amount of proteins and it takes a long time to reproduce
them, we are very hesitating to try
Hi Min,
If your proteins have cysteine residues, or you could introduce them
into the sequence, then you could label the interacting proteins with a
fluorescent label, run an SDS-PAGE and quantify the bands with a
fluorescent scanner. See for example Supplementary Figure 1C in Fronzes
et al.
Hi ,
yes as *Konstantin* said if u have cysteine residue in ur any one
of protein u can label that protein with Pyrene malemide or oregon
malemide or other fluorescence dye then remove the dye by desalting column.
titrate with second protein and do fluorescence anisotropy experiment
hi Min,
I think SPR(Surface plasmon resonance) should suit your situation. The
Biacore instrument I worked on required 200 µL of 10-50 µG protein(ligate)
for immobilisation and 150µL of various concentration of the other
protein(ligand) in the range of 50-1000 µg/ml. (depending on your situation
t
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