Re: [ccp4bb] Off topic about application of detergent on non-membrane protein crystallization

2012-06-14 Thread Artem Evdokimov
Yes, I had several cases like that - at least two published (PTPbeta and the TurboGFP structures). In the first case the protein needed a touch of beta-OG to stay soluble after the MBP tag was cut off (you have to wonder how many folks tried cutting off MBP, noticed that the product crashes and dec

Re: [ccp4bb] Coot installing problem

2012-06-14 Thread William G. Scott
If RedHat allows you to install a more current version of glibc, then do that. If not, switch to something like Ubuntu. William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Sant

[ccp4bb] SSRL Summer School

2012-06-14 Thread Smith, Clyde
The 2012 Structural Molecular Biology (SMB) Summer School will be held at SSRL between July 16th-20th and will focus on the following disciplines: Macromolecular Crystallography, Small Angle X-Ray Scattering, X-ray Absorption Spectroscopy, X-ray Fluorescence Imaging and X-ray Emission Spectrosco

[ccp4bb] X-FEL nanocrystallography SwissFEL

2012-06-14 Thread Schertler Gebhard
UNIVERSITÄT BASEL - PAUL SCHERRER INSTITUTE Professor in Nano-Diffraction of Biological Specimens The University of Basel and the Paul Scherrer Institute (PSI) in Switzerland invite applications for a Professor­ship in the area of Nano-Diffraction of Biological Speci­mens. We are considering app

[ccp4bb] Coot installing problem

2012-06-14 Thread Young-Jin Cho
Hi, I am using old RedHat now and am having some problem for using new version of coot. When I downloaded coot-0.6.2-binary-Linux-i386-rhel-5-python-gtk2.tar.gz, and simply untared and went to bin directory. When I typed as following it gave me following message: $ ./coot COOT_PREFIX is /home2/yjch

Re: [ccp4bb] Question on Symmetry Axis Notation Convention

2012-06-14 Thread Boaz Shaanan
Hi, Isn't there a notation 2 subscript 1 superscript x (one on top of the other) for "2-fold screw along x" for example? and likewise for y and z? Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: bshaa...@bgu.ac.il Phon

Re: [ccp4bb] Question on Symmetry Axis Notation Convention

2012-06-14 Thread Ethan Merritt
On Thursday, June 14, 2012 12:06:13 pm James Stroud wrote: > Hello All, > > I would like to discuss symmetry axes, but I'm not sure what the notation > convention is. For example, I'd like to say something about a 2(1) along the > x-axis, but the phrase "the 2(1) symmetry axis along x" is a bit

[ccp4bb] Question on Symmetry Axis Notation Convention

2012-06-14 Thread James Stroud
Hello All, I would like to discuss symmetry axes, but I'm not sure what the notation convention is. For example, I'd like to say something about a 2(1) along the x-axis, but the phrase "the 2(1) symmetry axis along x" is a bit cumbersome to repeat many times or to put in a table. So I'd like a

[ccp4bb] Fwd: [ccp4bb] Where to get a thermo-cycling controlled chamber

2012-06-14 Thread Jun Ma
Hi everyone, I think my first post did not clearly describe the machine I need. It's very kind of Zhijie and other repliers to reminde me this problem. So I post it again here, try to make it short and clear. Sorry for occupying the space of your mailbox twice. " Actually I need a chamber like a c

[ccp4bb] FFT for map variance

2012-06-14 Thread Frank von Delft
Proposition: what the paper below describes (map variance) would be rather useful to access routinely (e.g. in coot etc), given that it describes how to calculate by FFT. Discuss. (No, I've not read any of it carefully.) /Acta Cryst./ (2012). A*68*, 464-469 [ doi:10.1107/S010876731201577

Re: [ccp4bb] pROBLEM IN RUNNING dm

2012-06-14 Thread Appu kumar
thanks a lott sir It really helped me, Thank you for your kind and valuable information. Thank you Appu On 14 June 2012 20:53, James Holton wrote: > > This is a bug that arises when you import data with scalepack2mtz. > > The resulting mtz file is not "sorted", and apparently this makes DM > unh

Re: [ccp4bb] pROBLEM IN RUNNING dm

2012-06-14 Thread James Holton
This is a bug that arises when you import data with scalepack2mtz. The resulting mtz file is not "sorted", and apparently this makes DM unhappy in a rather uninformative way. However, if you run the data through CAD before inputting it into DM, then it works just fine. That is, just use the

Re: [ccp4bb] Aimless on MS Windows?

2012-06-14 Thread ronan . keegan
Dear Jan, There will be a Windows version of Aimless in the upcoming 6.3.0 release of CCP4 which is due very soon. We'll announce it on the bb when it's ready. Best wishes, Ronan -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jan Gebauer Se

[ccp4bb] How to not freeze crystals

2012-06-14 Thread Bosch, Juergen
is demonstrated if you jump to 1:20 on the timeline and wait for about 8 seconds. http://www.youtube.com/watch?v=spTmZrj9tv4 They didn't like the clear freeze of the crystal as it was invisible to their camera. But otherwise the video is considered advertisement for what we do. And it won an awar

Re: [ccp4bb] Sftools

2012-06-14 Thread Adam Ralph
Dear Michael,      In sftools you can generate a set of FreeR flags on only a subset of the reflections.  If you have a combined dataset (high and low relsoution), then select the high res refls, construct the FreeR set (unselected refls should not  be changed) and write out. sftools READ MTZFI

[ccp4bb] Aimless on MS Windows?

2012-06-14 Thread Jan Gebauer
Hi, I'm wondering, whether it's possible to run AIMLESS on Windows. Is there a binary version or instruction on how to compile if for windows? Would it work under windows anyhow? From time to time, I do crystallographic work under Windows (even more since phenix is ported). Regards, Jan

Re: [ccp4bb] Nvidia 3D Vision 2 - Centos5 - Coot

2012-06-14 Thread Laurent Maveyraud
In our case, the emitter is indeed connected to the graphic card with the 3 pin mini cable. The cable was included in the box. Le 14/06/2012 14:32, Sabuj Pattanayek a écrit : Do you not need the 3 pin mini din to 2.5mm vesa cable with the 3d vision v2 emitter under linux? I haven't found a mode

[ccp4bb] Faculty position in protein crystallography

2012-06-14 Thread Danny Huang
Lecturer/Senior lecturer position in X-ray crystallography located at the Beatson Institute for Cancer Research. The Beatson Institute for Cancer Research is committed to carry out world-class research into the biology of cancer and to help develop better treatments to improve the quality of lif

Re: [ccp4bb] pROBLEM IN RUNNING dm

2012-06-14 Thread Bosch, Juergen
If you run mtzdmp on your mtz file what labels do you get for FOM ? Enter the right label into DM and it won't complain. Jürgen Sent from my iPad On Jun 14, 2012, at 7:58, "Appu kumar" wrote: > Hello Dear all > I am trying running dm (density modification) > prog

Re: [ccp4bb] DLP Link vs. IR emitter based 3D projection

2012-06-14 Thread Sabuj Pattanayek
> 1)   a quadro 600 or 2000 and a DLP 3D Ready projector (3000-4000 Lumen) > with DLP Link Glasses? Here's the xorg.conf for (1) since I already gave you the hardware setup : http://nopaste.info/946bd53e3e.html . Enable generic active stereo in windows. You can basically drive this with any quadr

Re: [ccp4bb] Nvidia 3D Vision 2 - Centos5 - Coot

2012-06-14 Thread Sabuj Pattanayek
Do you not need the 3 pin mini din to 2.5mm vesa cable with the 3d vision v2 emitter under linux? I haven't found a model # for this kit that comes with this cable and last I checked nvidia store was out of stock on purchasing these cables separately, so we still purchase the v1 kits. On Thu, Jun

Re: [ccp4bb] pROBLEM IN RUNNING dm

2012-06-14 Thread Artem Evdokimov
Well, if you use the same coefficientsin fft, do you get a map out? Artem On Jun 14, 2012 6:58 AM, "Appu kumar" wrote: > Hello Dear all > I am trying running dm (density modification) > programe for density modification, but it giving error complaining about > density

[ccp4bb] pROBLEM IN RUNNING dm

2012-06-14 Thread Appu kumar
Hello Dear all I am trying running dm (density modification) programe for density modification, but it giving error complaining about density map. It says, dm: (WFO) No density in map! Check input FOM present and nonzero. Please suggest me how to overcome this proble

Re: [ccp4bb] Nvidia 3D Vision 2 - Centos5 - Coot

2012-06-14 Thread Laurent Maveyraud
Hi, on a Mandriva system, we had it working adding those two lines in the xorg.conf file, in the Section "Screen" : Option "Stereo" "10" Option "Stereo" "AllowDFPStereo" "1" The first line is mandatory with a LCD monitor. I have no idea what the second line means.. but it works with coot. ho

[ccp4bb] Nvidia 3D Vision 2 - Centos5 - Coot

2012-06-14 Thread S. Thiyagarajan
Dear CCP4 users Has anybody successfully configured NVIDIA 3D VISION-2 kit in CentOS-5.x and used COOT hardware stereo.  I am currently having Quadro 4000 graphics card and a 3D compatible Alienware monitor. I could configure these easily in windows but not getting much clues for CentOS-5.x.

[ccp4bb] DLP Link vs. IR emitter based 3D projection

2012-06-14 Thread mesters
Hi, I am looking for an affordable "3D Protein Cave" configuration (thus, no quadro >1500 US$ and no 3D stereoscopic projector > 1500 US$). At Schrödinger (Pymol) they successfully operate a viewsonic pro8450w, a high-end quadro and the vnidia 3D vision ready glasses (USB port based IR emitt