[ccp4bb] A SAXS REFINEMENT PUZZLE

We have collected SAXS curves for two multiprotein complexes (C1 and C2) where each complex is a specific combination of two kinds of Protomers (P1 and P2). P1 and P2 are well-folded structures and their atomic coordinates can be treated as rigid bodies. Both C1 and C2 can be thought of

Re: [ccp4bb] Improving microcrystals

I've had some success in the past following Terese Bergfors' advice: http://xray.bmc.uu.se/terese/tutorial3.html However, the last time I dealt with spherulites I just tuned the pH and it worked like a charm. Good luck, Jon 2012/8/25 RHYS GRINTER r.grinte...@research.gla.ac.uk Hi Samuel,

[ccp4bb] Dear ccp4

Dear CCp4 Thank you for all your and information suggestions regarding question Best Regards Rana

[ccp4bb] Small Molecule Effector Discovery for Hemoglobin

--Small Molecule Effector Discovery for Hemoglobin-- We seek an experienced scientist to join our multidisciplinary program directed at the discovery of new small molecule effectors of hemoglobin function. The successful candidate must

Re: [ccp4bb] Off-topic: Duet vectors with C-terminal GST tag

How about just co-transfecting with two different plasmids which have different selection markers? I've done it a lot, and it seems to work fine... JPK On Fri, Aug 24, 2012 at 10:39 PM, Lye, Ming ming_...@hms.harvard.eduwrote: Dear CCP4bb, We would like to co-express proteins under Se-Met

Re: [ccp4bb] Off-topic: Duet vectors with C-terminal GST tag

Hi Jacob, Thanks for your message! I was thinking about that too, but I wasn't sure if it was something that just sounded good in theory.. I guess the two different vectors should have different antibiotic resistance to select for the 'co-transformation.' Thanks so much! Ming

Re: [ccp4bb] sealing tapes

Hello Flip, I saw your post on the ccp4bb about UV transparent seals. Is it possible for you to send me the compilation that formulatrix has put together Thanks Hari Constellation Pharmaceuticals On Mon, Mar 7, 2011 at 4:41 AM, Flip Hoedemaeker f...@formulatrix.comwrote: Hi Jean-Luc, We

Re: [ccp4bb] Improving microcrystals

Samuel Clearly you should try the rMMS random microseeding approach where you add a seed stock made from the spherulites to a random screen. See refs: Acta Crystallographica section D63 (2007), 550–554. On-line at http://scripts.iucr.org/cgi-bin/paper?S0907444907007652 Cryst. Growth Des.,

[ccp4bb] Postdoctoral position

Dear all, I have an exciting postdoctoral position opened in my lab, available immediately. Please find attached the description and if possible, please forward to interested candidates. Best wishes, Svetla Svetla Stoilova-McPhie, PhD Assistant Professor, Department of Neuroscience and Cell

[ccp4bb] Meeting Announcement for the 4th Roadmap Meeting for Membrane Protein Structures and Complexes

*Meeting Announcement for the 4th Roadmap Meeting for Membrane Protein Structures and Complexes* The 4th NIH Roadmap meeting for Membrane Protein Structures and Complexes will be held at the San Francisco Westin Hotel in downtown San Francisco on Wednesday November 28th 2012, 8:00 AM through