You might try mercury soaks instead. Might give you 15 minute answers to your
questions.
HNY!
Ho
Sent from my iPad
On Dec 26, 2012, at 4:00 PM, CCP4BB automatic digest system
wrote:
> There is 1 message totaling 34 lines in this issue.
>
> Topics of the day:
>
> 1. SeCys usage for SAD
>
Dear Linda, If your molecule is not very big, your cell is not very big, you
have an approach to modern in-house X-ray facility, your crystal diffract
reasonably well ( :-\ ) you can try S phasing. If you will be able to measure
accurate data, S anomalous phasing at 1.5417 Angstrom of Cu radiati
Dear all:
I encountered problem in adding hydrogen to my model including protein and
DNA components using phenix.ready_set or phenix.reduce. Hydrogens are only
added to protein but not to DNA.
Any ideas of fixing the problem?
Many thanks and best regards
sheng
Dear All,
I have read a recent paper by Salgado et al about using non-auxotrophic E.coli
to incorporate SeCys into recombinant protein for phasing purposes. Does
anyone have a source for Selenocysteine? I have also seen a paper by Schaefer
et al which uses nitric acid treated elemental Se for
