Re: [ccp4bb] SeCys usage for SAD

2012-12-26 Thread Ho Leung Ng
You might try mercury soaks instead. Might give you 15 minute answers to your questions. HNY! Ho Sent from my iPad On Dec 26, 2012, at 4:00 PM, CCP4BB automatic digest system wrote: > There is 1 message totaling 34 lines in this issue. > > Topics of the day: > > 1. SeCys usage for SAD >

Re: [ccp4bb] SeCys usage for SAD

2012-12-26 Thread Felix Frolow
Dear Linda, If your molecule is not very big, your cell is not very big, you have an approach to modern in-house X-ray facility, your crystal diffract reasonably well ( :-\ ) you can try S phasing. If you will be able to measure accurate data, S anomalous phasing at 1.5417 Angstrom of Cu radiati

[ccp4bb] adding H to DNA

2012-12-26 Thread cuisheng2007
Dear all: I encountered problem in adding hydrogen to my model including protein and DNA components using phenix.ready_set or phenix.reduce. Hydrogens are only added to protein but not to DNA. Any ideas of fixing the problem? Many thanks and best regards sheng

[ccp4bb] SeCys usage for SAD

2012-12-26 Thread Linda Olson
Dear All, I have read a recent paper by Salgado et al about using non-auxotrophic E.coli to incorporate SeCys into recombinant protein for phasing purposes. Does anyone have a source for Selenocysteine? I have also seen a paper by Schaefer et al which uses nitric acid treated elemental Se for