Re: [ccp4bb] Data mining interactions in the PDB

2013-10-28 Thread Prof. K. Sekar
How about this server http://iris.physics.iisc.ernet.in/psap/ > On 10/29/2013 03:23 AM, Katherine Sippel wrote: >> Hi all, >> >> I was wondering if anyone knew of a software or server to mine the PDB >> for a specific class of interactions? I've tried PDBeMotif without much >> luck and I though

Re: [ccp4bb] Conflicting Qt on OS X 10.6

2013-10-28 Thread Eugene Krissinel
Yes, this is a result of some imperfectness in shared build (6.3.0 was static). The bug should have been fixed in updated release bundles, and also in the recent update. Eugene From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Dmitry Rodionov

Re: [ccp4bb] Data mining interactions in the PDB

2013-10-28 Thread Francois Berenger
On 10/29/2013 03:23 AM, Katherine Sippel wrote: Hi all, I was wondering if anyone knew of a software or server to mine the PDB for a specific class of interactions? I've tried PDBeMotif without much luck and I thought I'd check to see if there was an alternative before I go re-inventing the whee

Re: [ccp4bb] Data mining interactions in the PDB

2013-10-28 Thread Tom Oldfield
Katherine I am not exactly sure what sort of tool you would like to use, but there are a couple of options found here... http://www.ebi.ac.uk/pdbe-as/pdbetemplate/ The last option DB search http://www.ebi.ac.uk/pdbe-as/pdbesearch/ allows you upload a fragment of structure - a small number of r

Re: [ccp4bb] Biacore/SPR

2013-10-28 Thread Tom Peat
As Juergen has mentioned, both the T200 and the BioRad ProteOn (XPR36) are good options (we have both). The XPR36 is a higher throughput machine and can also be used for small molecule work (not quite as sensitive as the T200, but more sensitive than we had anticipated and certainly higher throu

Re: [ccp4bb] Data mining interactions in the PDB

2013-10-28 Thread Ho,Shing
I don't know whether your question is specifically for protein-ligand interactions, protein-protein interactions, internal protein interactions, etc., but one place to start is to look at some of the database tools already available on-line, including, for example, CREDO from Tom Blundell's grou

Re: [ccp4bb] Biacore/SPR

2013-10-28 Thread Xiaodi Yu
Another option is BLItz which is cheaper and uses interferometry. Dee Date: Mon, 28 Oct 2013 11:05:33 -0400 From: jubo...@jhsph.edu Subject: Re: [ccp4bb] Biacore/SPR To: CCP4BB@JISCMAIL.AC.UK BiaCore 3000 or if you can afford the T200.Proteon XPR36 would be a cheaper Option and should work as w

[ccp4bb] Data mining interactions in the PDB

2013-10-28 Thread Katherine Sippel
Hi all, I was wondering if anyone knew of a software or server to mine the PDB for a specific class of interactions? I've tried PDBeMotif without much luck and I thought I'd check to see if there was an alternative before I go re-inventing the wheel. Cheers, Katherine -- "Nil illegitimo carboru

Re: [ccp4bb] Conflicting Qt on OS X 10.6

2013-10-28 Thread Dmitry Rodionov
Thanks for the suggestions! I just opened a new terminal window, and it appears that problem had disappeared (by itself?). Sorry for the false alarm- I'll go see a mental health professional. Best regards, Dmitry On 2013-10-28, at 12:49 PM, William Scott wrote: > It looks like it should

Re: [ccp4bb] Conflicting Qt on OS X 10.6

2013-10-28 Thread Phil Evans
I had this problem last week but it seems to have been solved by reinstalling the ccp4 6.4.0 package today, slightly updated I believe There was message from Eugene Krissinel which may be relevant to this Phil On 28 Oct 2013, at 16:00, Dmitry Rodionov wrote: > Good day! > > I'm having proble

Re: [ccp4bb] Conflicting Qt on OS X 10.6

2013-10-28 Thread William Scott
It looks like it should be doing the right thing, i.e., zsh-% otool -L qtrview qtrview: @executable_path/../Frameworks/QtWebKit.framework/Versions/4/QtWebKit (compatibility version 4.9.0, current version 4.9.3) @executable_path/../Frameworks/QtXmlPatterns.framework/Versions/4/Q

[ccp4bb] Announcing the Eighth International Workshop on X-ray Radiation Damage to Biological Crystalline Samples, April 10-12 2014, Hamburg

2013-10-28 Thread Elspeth Garman
The Eighth International Workshop on X-ray Radiation Damage to Biological Crystalline Samples will be held at the EMBL and DESY, Hamburg, Germany from April 10th (13:00) to April 12th (13:00) 2014. This series of workshops was originally concerned with the effects of radiation damage during i

[ccp4bb] Conflicting Qt on OS X 10.6

2013-10-28 Thread Dmitry Rodionov
Good day! I'm having problems with qtrview (on OS X 10.6): it crashes or hangs after spitting out a screenful of messages like objc[2975]: Class QCocoaMenu is implemented in both /Applications/ccp4-6.4.0/bin/../Frameworks/QtGui.framework/Versions/4/QtGui and /Library/Frameworks/QtGui.framework

Re: [ccp4bb] Biacore / SPR

2013-10-28 Thread Badreddine
Dear Dong, Biacore T200 is the best choice for protein/small molecule interaction analysis. the Biacore 3000 works perfectly for protein-protein interactions. hope that help you. Badr -- Dr Douzi Badreddine Architecture and Function of Biological Macromolecules (AFMB), CNRS UMR7257 163 Avenue d

Re: [ccp4bb] Biacore/SPR

2013-10-28 Thread Bosch, Juergen
BiaCore 3000 or if you can afford the T200. Proteon XPR36 would be a cheaper Option and should work as well. Jürgen On Oct 28, 2013, at 10:54 AM, Gang Dong wrote: We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:jubo.

Re: [ccp4bb] Biacore/SPR

2013-10-28 Thread Gang Dong
We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:jubo...@jhsph.edu] Sent: Monday, October 28, 2013 3:17 PM To: Gang Dong Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Biacore/SPR Protein-protein interaction? p

Re: [ccp4bb] Biacore/SPR

2013-10-28 Thread Bosch, Juergen
Protein-protein interaction? protein-small molecule interactions ? Epitope mapping of mABs ? Could you specify what you would like to do, as different models are good for different things. Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with

[ccp4bb] Biacore/SPR

2013-10-28 Thread Gang Dong
Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang

[ccp4bb] Off-topic: GST binding to cleaved protein - problem with seperating

2013-10-28 Thread Read, Jon
Just a quick off-topic question which people here may have experience in: We have a protein which expresses with a GST tag. It has much poorer expression without. When the tag is cleaved, the protein does not crash out but we are having difficulty separating it as the proteins seem to stick toge

Re: [ccp4bb] Unusual electron density - any guesses??

2013-10-28 Thread Eleanor Dodson
Well I would start by flipping the carbonyl oxygen then see if the side chain can use the density - what are the B values for the neighbouring stuff? Eleanor On 25 October 2013 19:00, Patel, Joe wrote: > Is that a glycine in the sequence next to the Glu/Gln? Have you tried > building a 50% occ