One or more ingredients in the original hit (which you use to suspend the
seed crystals in) can indeed be essential, and may be all you need to get
nice crystals. In D'Arcy's original paper you will see that he needed Ca2+
to get nice crystals of one of his proteins. The same is true in
Hello Everyone,
I am currently trying to phase a structure with an asymmetric unit
predicted to contain 20-24 monomers (space group P1). The native crystals,
while beautiful in appearance (see attached), only diffract to ~3.4-3.0
angstroms at best, and SeMet-derived crystals grow with poor
Postdoctoral Fellow in Structural Virology
Institute for Molecular Virology
University of Minnesota‐Twin Cities
Minneapolis, MN 55455
Web: www.virology.umn.edu
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A Postdoctoral Fellow is being sought for a collaboration involving the
application of cryo‐electron tomography
Dear Colleagues:
Posdoctoral position at the School of Biological Sciences University of Auckland
We are seeking a well-qualified and highly motivated candidate for the position
of a postdoctoral Research Fellow to carry out structure/function studies of
anti-feeding prophage (Afp) using
Hi Chris,
It would be nice to have a wee bit more information. Is 3.4-3.0 angstroms from
a home source
or synchrotron? What are the crystallization conditions for both the native
and SeMet crystals?
Did you see the SeMet crystals with the native crystals. Have you tried MMS
with the native
You sure about this space group? 24 monomers in P1 is unusual (at least to me)
F
On Jan 18, 2014, at 9:14 AM, Chris Fage cdf...@gmail.com wrote:
Hello Everyone,
I am currently trying to phase a structure with an asymmetric unit predicted
to contain 20-24 monomers (space group P1). The
Francis, It can happened
We have (not yet published) P1 with 24 molecules. When we cut His-tag we get
P1 with 32 molecules.
In our case we believe it is dictated by very strong interaction between two
monomers, and strong interaction between dimers with build a flattish tetramer.
Probably
