[ccp4bb] Postdoctoral Position Protein Crystallography available at The Institute of Cancer Research, London, UK

2014-10-16 Thread Rob Van Montfort
The Institute of Cancer Research, London, is one of the world’s most influential cancer research institutes, with an outstanding record of achievement dating back more than 100 years. We provided the first convincing evidence that DNA damage is the basic cause of cancer, laying the foundation

[ccp4bb] AW: [ccp4bb] Trimming of carbohydrate chains ---- Summary

2014-10-16 Thread Herman . Schreuder
Dear Bulletin Board, as usual with the bulletin board, I quickly got a number of very useful suggestions, which I summarize below. Mick Blaise, Alisa Glukhova and Jacinto suggested to use endo F. Mick said that in his case always some sugar was left, which in my case would keep my protein in

Re: [ccp4bb] Command line tool for RMSD calculation

2014-10-16 Thread MARTYN SYMMONS
Mentioned recently but worth mentioning again? I have used Chimera rmsd from its own command line (find the command line under the 'favorites' tab) - it is scriptable. https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/rmsd.html Must have equal number of atoms matched - use selection to make

[ccp4bb] Your top choice of crystal screens!

2014-10-16 Thread xaravich ivan
Dear Xtallographers, As the budget becomes tighter it is difficult to get hold of all the crystal screens one would want to try, to crystallize a protein. What in your opinion/experience is/are the first few commercial crystal screens you would try? ( or what are the ones you routinely start

[ccp4bb] problem with PEE.cif

2014-10-16 Thread Edward A. Berry
(for developers or whoever manages the .cif libraries. Is there a separate list?) PEE has been used (mainly by me) for the natural lipid PE (1,2-Distearoyl-sn-glycero-3-phosphoethanolamine), but the current PEE.cif specifies the wrong chirality about the glycerol C2 atom, giving un-natural

Re: [ccp4bb] Your top choice of crystal screens!

2014-10-16 Thread xaravich ivan
Thanks to all for your suggestions. I really appreciate your time. warm regards, Subhendu On Thu, Oct 16, 2014 at 9:57 AM, xaravich ivan xaravich.i...@gmail.com wrote: Dear Xtallographers, As the budget becomes tighter it is difficult to get hold of all the crystal screens one would want to

Re: [ccp4bb] Your top choice of crystal screens!

2014-10-16 Thread jens j birktoft
Subhendu, Could you please share what you learned.This is something we all(?) want to know more about. Cheers Jens On Thu, Oct 16, 2014 at 5:55 PM, xaravich ivan xaravich.i...@gmail.com wrote: Thanks to all for your suggestions. I really appreciate your time. warm regards, Subhendu On Thu,

Re: [ccp4bb] incorrect R-factor calculation in sftools

2014-10-16 Thread Paul Emsley
On 16/10/14 19:31, George Devaniranjan wrote: Could be a very dumb question, how does this affect COOT I wonder. I don't see how this could affect Coot. Sometimes when I use it to look at a PDB, it highlights some maps as unreliable Of course its relying on EDS +/-5 of the reported value

Re: [ccp4bb] Your top choice of crystal screens!

2014-10-16 Thread xaravich ivan
Hi everyone, Several of you wanted to know what kind of suggestions I received. Attached you will find all the replies. Thanks On Thu, Oct 16, 2014 at 9:57 AM, xaravich ivan xaravich.i...@gmail.com wrote: Dear Xtallographers, As the budget becomes tighter it is difficult to get hold of all

[ccp4bb] Some spots left for PSDI2014, detailed program now available

2014-10-16 Thread Anna Gardberg
*PROTEIN STRUCTURE DETERMINATION IN INDUSTRY 2014* A detailed program http://cc-special.merck.de/PSDI2014/program.html for the 22nd PSDI is now available at the conference website http://cc-special.merck.de/PSDI2014/. The conference will take place at the Hotel Miragem in Cascais, Portugal, a

[ccp4bb] comparisons - views

2014-10-16 Thread Dean Derbyshire
Apologies for the slightly off topic... thought this was the best way to get views from a wide (relevant) audience. Any views on differences - pros and cons - and experiences with: Mosquito; Gryphon and NT8? And similarly with Minstrel and Rock imager. Related to that last 'comparison' what's