Dear CCP4 users,
Recently, I got some datasets with weak anomalous Br signal. I tried to
merge them according to Q. Liu et al Science 336, p1033 (2012). I am using
the script multiscale@SSRL. The merged dataset has WEAKER anomalous
signals.
Liu et al used SCALA for scaling and merging while
Instead of Br which has a weak signal at its K edge, why not use Iodide at
some wavelength (or energy) where it gives a stronger signal? The longer
wavelength used will help with spot spatial resolution as well.
One may be fighting radiation damage, too.
One may be fighting a moving (vibrating?)
In my practice, I never worried about the skin. You need extra care while you
mount the crystal. In my case I have used the skin to mount my crystals without
any problem. In fact it was much helpful. While mounting, you should not take
the crystal covered with the skin.
Best
Syed
Dear Gerard, Eleanor et al.,
In the graphs of Rmeas vs. batch number (and I or I/sigma vs. batch
number), everything looks roughly constant, which made me think that
significant radiation damage was not the issue--but are you saying that
radiation damage might also (or instead) produce a smiley
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Hi Charles,
I don't know what multiscale does. Probably the right thing. If the
anomalous signal is weaker after scaling of multiple datasets,
non-isomorphism might be at fault. Try Blend to scale your datasets.
Blend is part of ccp4 and gives good graphical diagnostic feedback.
Andreas
Hi Veronica,
as others have said, at RT the radicals travel much further than at cryo
temperature, so your lateral shifts are probably not sufficient. But it is not
clear to me that radiation damage is to blame at all. To me it sounds like you
might have to mask your beamstop better, or you
