[ccp4bb] Postdoc position at Houston TMC area

Dear All, A CPRIT funded postdoctoral position on single particle cryo-EM is available at Houston Texas Medical Center. The project will be led by the Dr. Mien-Chie Hung group at the University of Texas MD Anderson and will involve collaborations with Dr. Zhao Wang group (Baylor College of

Re: [ccp4bb] Rmergicide Through Programming

I think the confusion here is that the "multiplicity correction" is applied on each reflection, where it will be an integer 2 or greater (can't estimate variance with only one measurement). You can only correct in an approximate way using using the average multiplicity of the dataset, since it

Re: [ccp4bb] Rmergicide Through Programming

Not so fast: First of all, I cannot remember having ever come across a paper reporting a multiplicity of around 1, and if there are such cases, they are so rare that they are not worth accounting for, and should raise eyebrows in the first place, cast into doubt all of the statistics, let

Re: [ccp4bb] Rmergicide Through Programming

James, I cannot follow you. "n approaches 1" can only mean n = 2 because n is integer. And for n=2 the sqrt(n/(n-1)) factor is well-defined. For n=1, neither contributions to Rmeas nor Rmerge nor to any other precision indicator can be calculated anyway, because there's nothing this

Re: [ccp4bb] Rmergicide Through Programming

Okay, that /is/ a strong answer: Rmeas has too many infinities for comfort. Thanks, very instructive yet again! phx On 07/07/2017 18:57, James Holton wrote: I happen to be one of those people who think Rmerge is a very useful statistic. Not as a method of evaluating the resolution limit,

Re: [ccp4bb] Rmergicide Through Programming

I happen to be one of those people who think Rmerge is a very useful statistic. Not as a method of evaluating the resolution limit, which is mathematically ridiculous, but for a host of other important things, like evaluating the performance of data collection equipment, and evaluating the

Re: [ccp4bb] Any suggestions?

Dear all, My apologies for not being able to see the pictures. I have attached them as PDFs here but not sure if you will be able to get the files. Anyway, many thanks to all who have reply via ccp4bb or privately with suggestions. Indeed the protein is purified from the natural source so

Re: [ccp4bb] Any suggestions?

Yes no images came through, however on looking at your crystallisation conditions for clues, is there anything in the protein prep that could have been carried through, even from the early stage buffers, or cell growth media? We once had a detergent in the cell lysis stage that was removed from

Re: [ccp4bb] Any suggestions?

"Dr. Isabel De Moraes" writes: > Any suggestions regarding to the positive densities? > Your pictures didn’t seem to make it as attachments, at least as received here. BW, -- Ian ◎

[ccp4bb] Any suggestions?

Any suggestions regarding to the positive densities? The crystallisation condition only has NaCl and CaCl [cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk] [cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk] Best regards, Isabel -- This e-mail and any attachments may

Re: [ccp4bb] CCP4i2 error message manual coot

Me. On 7 July 2017 at 10:38, Bernhard Rupp wrote: > Dear ccp4i2 developers, > > > > I am trying to run manual coot for sugar correction after privateer. > > Privateer works fine, but the subsequent manual coot (button) > > starts but exits with a short error message

[ccp4bb] CCP4i2 error message manual coot

Dear ccp4i2 developers, I am trying to run manual coot for sugar correction after privateer. Privateer works fine, but the subsequent manual coot (button) starts but exits with a short error message and plenty of error logs I do not completely comprehend. Coot itself also works fine.