Re: [ccp4bb] Interesting pattern on a crystallization drop

Neat! Looks like multiple adjacent bubbles that were initially touching but eventually shrunk down to the central cores - the connectors are protein filaments (skin on the bubbles) left over from when bubbles had contact points. Artem On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing,

Re: [ccp4bb] Interesting pattern on a crystallization drop

Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition is found in both Index and JCSG screens as well as Classics II. Bevan Marshall Staff Scientist | Collaborative Crystallisation Centre Manufacturing CSIRO E bevan.marsh...@csiro.auT +61

Re: [ccp4bb] Interesting pattern on a crystallization drop

Dear Beatriz, Nice drops :-)) Could it be that there is a reaction going on in these drops ? The conditions are quite "exotic" with possibilities of coordination or oxydoreduction (Co2+/Co3+) or polymerization... Do you have reductants with the protein buffer ? Is the protein an enzyme or a

Re: [ccp4bb] Interesting pattern on a crystallization drop

Dear all, some more details: drops were set up by a robot (Mosquito), 200 nL prot+ 200 nL reservoir on a 96-Well sitting drop iQ Plate (TTPlabtech). They are not really bubbles. I have the impression that the lines are all around creating these "empty rounds" (maybe "exploded bubbles"?)

Re: [ccp4bb] Interesting pattern on a crystallization drop

Hi Beatriz, Were the drops set up by hand or by a robot? If the latter, what kind of robot? are they hanging drops or sitting drops, and do the bubbles form on the glass surface of at the bottom of your drop? AK On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes

Re: [ccp4bb] Interesting pattern on a crystallization drop

It's a crystal with a very large lattice. JPK + Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 Desk: (571)209-4000 x3159 Cell: (301)592-7004

Re: [ccp4bb] Interesting pattern on a crystallization drop

I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing that the protein foams quite a bit, and that there were 6 (or four) more or less equal size bubbles that took up almost the whole drop when it was set up. Bubbles tend to pack quite efficiently

Re: [ccp4bb] Interesting pattern on a crystallization drop

Hi Philippe I do not know yet if it is reproducible. And protein function has nothing to do with polymerization or something ... it is a RNA interacting protein, small and usually monomeric. I will try to find a better one for the April 1st... but it will be hard! Best regards, Beatriz

Re: [ccp4bb] Interesting pattern on a crystallization drop

Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes a écrit: In four days from now, I (everyone) would have taken this as a Photoshop joke... Quite amazing! Is it reproducible? What is the protein? Tubulin ? ::)) Philippe Dumas > Dear all, > > > I would like to share with you a

[ccp4bb] CCP-EM Spring Symposium - registration closes this Friday (29th March)

Dear all, Just a quick reminder that registration for the Spring Symposium will close this Friday (29th March). Full details and talk titles can be found here: http://www.cvent.com/d/xbqsqk The Spring Symposium aims to provide a forum to highlight state of the art developments in

[ccp4bb] Postdoctoral fellowships to study Type 4 Secretion System at Umeå University, Sweden

Dear all, The Berntsson lab at the department of Medical Biochemistry and Biophysics at Umeå University, Sweden, is looking to recruit up to two postdoctoral fellows (2-year fellowships, funded by the Kempe Foundation) to study the structural and functional aspects of Type 4 Secretion Systems.

Re: [ccp4bb] Some kind of bug, Can't perform invert selection through file menu in subset selection for micrographs

Dear Suchi, For questions on Relion (and other EM related things) best to try the CCP-EM mailing list (and whilst your reposting I'll try and reproduce your bug on my mac ): https://www.jiscmail.ac.uk/CCPEM Best wishes, Tom From: CCP4 bulletin board on