Job description
Dr. Julien’s laboratory at The Hospital for Sick Children Research Institute
(Toronto, Canada) is looking for two Postdoctoral Fellows to join our
Structural Biology team. By using a combination of biochemical, biophysical,
immunological and structural techniques, the successful
When the JCSG was in production, we would routinely collect X-ray emission
spectra from our samples to look for metals. We also saved all crystals
after data collection and if we saw extra anomalous peaks in the maps
during refinement, we would put the crystal up again and collect
quick low
Many thanks for all of the suggestions. Ni2+ or Zn2+ seem to be the most
likely culprits, but I plan to carry out a fluorescence scan at my next
beam session.
While it’s interesting that others have observed crystallization-driven
formation of disulfides, the binding of a metal ion at the given
Postdoctoral Researcher
Lawrence Berkeley Laboratory
Job ID: 89267
Division: Molecular Biophysics & Integrated Bioimaging (Computational
Crystallography Initiative)
Date Opened: 12/23/2019
Summary: Berkeley Lab’s Molecular Biophysics and Integrated Bioimaging
Division has an opening for a
This conversation brings up the question of whether it would be good practice
to always do a fluorescence scan of a crystal sample, whether it is known to be
a metalloprotein or not...
Energy dispersive X-ray fluorescence spectra (accessible at most synchrotron
sources at this point) can often
Hi Chris,
it could be all Ni besides Zn. I've seen Ni carried over from the initial
metal affinity chromatography I presume.
Cheers Christian
Chris Fage schrieb am Di., 21. Jan. 2020, 21:23:
> Thanks to Guenter and Eleanor for their replies. I mentioned that there is
> not adequate space for
The BCA Spring Meeting is fast approaching and the BSG committee are still
looking for nominations for the Early Career Prize. Anyone who has recently
obtained a personal fellowship, a lectureship or equivalent in the field of
Structural Biology will be considered, though preferably with an
Hi all,
Just to conclude on this - ARCIMBOLDO via CCP4 in coiled coil mode worked quite
nicely in the end now that I finally had time to work through it - took a few
days with 8 x 20 aa peptides as the search set
on my old iMac but the end result was fine :). So didn't have to try other
options
Hello,
You could run Image Quant TL in a VM (parallels, vmware or virtual box).
https://bmi.cchmc.org/resources/software/imagequant-tl
For this appliation (arrays), we use an old program called VisualGrid
that is no longer available and run it an isolated XP VM via virtual box
(in linux).
