I would try iodoacetamide.
Sent from my iPhone
> On Feb 25, 2021, at 7:58 PM, David wrote:
>
> EXTERNAL EMAIL – Use caution with any links or file attachments.
>
> I would like to know how to block activated groups on Thiol-activated
> Sepharose 4B (after coupling a peptide). The
Dear all,
We have a postdoc position available at Washington University in St. Louis.
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Please see attachment for
I would like to know how to block activated groups on Thiol-activated Sepharose
4B (after coupling a peptide). The immobilized peptide will be used later to
purify/identify which antibody binds to this peptide.
For CNBr-activated sepharose I use Tris or ethanolamine to block the remaining
Well - you are a long way from a solution!
All those rotation angles are close to each other..
What is the cell?
How similar is the model to your structure?
Have you looked at the self rotation function?
Eleanor
On Thu, 25 Feb 2021 at 20:54, Muhammad Bashir Khan wrote:
> *Hello everyone;*
>
>
*Hello everyone;*
*I collected data set which can be easily processed in a space grouop
P21 (P1211)*
*at approx 3.7A. I procedeed with MR using phaser in phenix which give
a solution with two copies.
*
** There were 8 solutions
** Pdb and/or Mtz files have been written with results for 1 of
*Dear Saif*
*Hope you are doing well and safe!*
1) How much change in Tm (ΔTm) in a thermal shift assay is considered to be
significant ?
*As it has already been mentioned there is no specific cutoff for deltaTm
to be considered significant. DeltaTm depends on many factors, including
the type
Hi Saif,
If your goal is to perform co-crystallization, I am completely agreed with
David's suggestions. Delta Tm does matter in the co-crystallization but
that's not always the case. I have some experience with protein complexes
where I successfully co-crystallized by using really high molar
Hello,
we had an interesting case in the lab many years ago... Using the
shift-assay, a student managed to identify conditions that markedly
stabilized the protein of interest. To cut a long story short, in the
end it turned out conditions were identified that gave monomers while
the
Hi Saif,
Whilst in very general terms, ∆Tm does correlate with binding affinity (there
will of course always be exceptions to this rule), I don't think there is a
cutoff beyond which you know that co-crystallisation is feasible. The degree of
stabilisation will depend very much on the system
Hello everyone,
1) How much change in Tm (ΔTm) in a thermal shift assay is considered to be
significant ?
2) A negative ΔTm infers that the compound is making the protein unstable.
In such a case, will the co-crystallization be difficult or just impossible
or on the contrary it shouldn't matter
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---
Postdoctoral position in Structure of Plant Hormone Transporting Membrane
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