Re: [ccp4bb] ccp4 installation problem

Hi Robert, On Fri, Sep 16, 2022 at 4:11 PM Kay Diederichs wrote: > Your real problem seems to be the Qt installation; I think that the error > message > qt.qpa.plugin: Could not load the Qt platform plugin "xcb" in "" even though > it was found. > This application failed to start because no Qt

Re: [ccp4bb] ccp4 installation problem

Hi Kay Thanks again and for more insight and feedback on the Qt installation. Reply in blue text below. Take care, Rob -Original Message- From: Kay Diederichs Sent: Friday, September 16, 2022 4:11 PM To: CCP4BB@JISCMAIL.AC.UK; Robert Gampe Subject: Re: ccp4 installation

Re: [ccp4bb] ccp4 installation problem

Hi Robert, you are welcome. It seems that you own (rgampe.rgampe) the directory /software/ccp4-8.0 . You should revert the ownerships in /software/ccp4-8.0 from root.root with sudo chown -R rgampe.rgampe /software/ccp4-8.0 so that in the future you don't need sudo for updates. Your real

Re: [ccp4bb] ccp4 installation problem

Hi Robert, hmm, more ideas: a) check if CCP4 directory is writable, and who the owner is: mount | grep software sudo touch /software/ccp4-8.0/deleteme ls -ld /software/ccp4-8.0 ls -l /software/ccp4-8.0/deleteme sudo rm /software/ccp4-8.0/deleteme b) try sudo su ccp4um c) try sudo -i

Re: [ccp4bb] ccp4 installation problem

Hi Robert, two points - a) before you tried to update, did you source /where/ever/ccp4/bin/ccp4.setup-sh ? The above applies to bash; for (t)csh you need to source /where/ever/ccp4/bin/ccp4.setup-csh That sets the PATH environment variable such that you don't always have to specify the

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Oleksiy, If the Akta Start is just for loading affinity columns (without air sensor or software), why not to get a Cyvita peristaltic pump for £3k rather than £9k? They

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

We have a AKTA start and a AKTA Pure. Don’t like the AKTA start at all. The software is buggy (command not performed correctly as it should do) but the touch screen is OK. By now I have changed two failed/leaking valves and the UV detector for the AKTA start with very light usage. AKTA start

Re: [ccp4bb] ccp4 installation problem

Hi Robert, Concerning PROBLEM 1: have you tried sudo ccp4um or sudo ccp4i ? HTH, Kay P.S. didn't find Andrey Lebedev's reply on August 30 that you refer to On Fri, 16 Sep 2022 14:11:34 +, Robert Gampe wrote: >Hello Marcin Pedro and the CCP4 community, > > > >PROBLEM 1: I have the same

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Eike, Instead of AKTA Pure I purchased a combo of AKTA Go (€24k with air sensor) and AKta start (€9k with frac collector). It provides two parallel workstations with AKTA start handling the dirty job of loading lysates onto affinity cartridges and the GO doing clean SEC stages. For multiple

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Stephan, You’re mostly correct, however, gradients made on instrument with one pump are less reliable. Thank you. Vaheh From: CCP4 bulletin board On Behalf Of Stephan Rempel Sent: Friday, September 16, 2022 7:38 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic: experience

Re: [ccp4bb] Problem downloading models maps in from PDBe in Coot

Hopefully 0.9.8.4 should fix that. Paul. On 16/09/2022 13:54, Jan Dohnalek wrote: This has been quite variable in (several?) last versions of Coot. I have some three latest versions available and when one does not work I start another one. Sad but true. Jan On Thu, Sep 15, 2022 at 11:39

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Eike, we bought a Biorad back in 2005 or so, an Akta Purifier in 2011 and recently an Akta Go (2020). All work(ed) well, the latter two are in use. Don't know about the Biorad, it's in Santiago de Compostela. Both the Biorad and Purifier have been used a lot, the Akta Go not that much so

[ccp4bb] Open Postdoc position: 'Imaging at extreme intensities using XFEL' (Hamburg)

Dear All, We'd like to advertise an open Postdoc position in the project "Imaging at extreme intensities using XFEL"  with Dr. Saša Bajt within our Cluster of Excellence 'CUI: Advanced Imaging of Matter' at the Universität Hamburg, Germany. Please see here for further information:

Re: [ccp4bb] Problem downloading models maps in from PDBe in Coot

This has been quite variable in (several?) last versions of Coot. I have some three latest versions available and when one does not work I start another one. Sad but true. Jan On Thu, Sep 15, 2022 at 11:39 AM Robbie Joosten wrote: > Not sure if this is a PDBe bug or a Coot bug (or a

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi, I had some experience for about 2-3 years with the BioRad systems before I moved on and they worked just fine like the AKTAs. At the time, the price point was a huge plus over the AKTA Pure and in my opinion for protein purification there was no reason to fork out the money for an AKTA

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hello Eike, A couple of comments on the AKTA Pures (while I have not experience with the BioRad systems). We have a couple of AKTA Pures and we (and another lab close to us) had a catastrophic failure of the 3-wavelength detector "block". Price tag for the replacement part (apparently a black

[ccp4bb] CLOSING SOON: DLS/CCP4 data analysis workshop 2022

Hi all, The application period for the DLS/CCP4 workshop closes 17:00 (UK time) on Tuesday the 20th. Don't leave it to the last minute to apply! :) Cheers -- David On Tue, 13 Sept 2022 at 12:02, David Waterman wrote: > Hi folks, > > A reminder: there is one week left to apply for this year's

Re: [ccp4bb] PDBx-format utilities

We have our internal PDBCOP program that extracts all the info you need when refining a structure, i.e. extremes of B values, of occupancies, listing of all alternatives, all compounds found in the file and an option to provide the largest differences between two versions of the same structure,

[ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Dear all, I am considering to purchase a chromatography system for routine protein purification. The device is supposed to be used in a multi-user environment, hence ease of use, ease of training and ease of maintenance is important. I am rather looking for a robust system people like to use